Difference between revisions of "YBR225W"
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| − | The graph above shows the growth rate for the previously listed strains and the relative level of NaCl concentration. Comparing the | + | The graph above shows the growth rate for the previously listed strains and the relative level of NaCl concentration. Comparing the YBR225W doubling times in 0mM and 750mM NaCl, there is a no effect on the growth rate. This effect has no influence on the knockout strain's (YBR225W) growth. |
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===[[UW-Stout/Caffeine_SP21|Caffeine Group 1]]=== | ===[[UW-Stout/Caffeine_SP21|Caffeine Group 1]]=== | ||
Revision as of 10:00, 27 April 2021
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| Systematic name | YBR225W |
| Gene name | |
| Aliases | |
| Feature type | ORF, Uncharacterized |
| Coordinates | Chr II:670627..673329 |
| Primary SGDID | S000000429 |
Description of YBR225W: Putative protein of unknown function; non-essential gene identified in a screen for mutants affected in mannosylphophorylation of cell wall components[1][2]
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Contents
Community Commentary
About Community Commentary. Please share your knowledge!
This gene is part of the UW-Stout Orphan Gene Project. Learn more here.
Growth Curve
In a BY4735 background, knocking out YBR225W does not seem to have any effect on the strain's growth rate. In this assay, the BY4735 strain's doubling time was 162 minutes, while the YBR225W knock-out strain's doubling time was 156 minutes. (These doubling times are the means of three experiments.)
Salt Concentration (NaCl)
0mM NaCl conc. BY4735 strain's doubling time: 169 minutes
0mM NaCl conc. YBR225W strain's doubling time: 224 minutes
750mM NaCl conc. BY4735 strain's doubling time: 294 minutes
750mM NaCl conc. YBR225W strain's doubling time: 226 minutes
The graph above shows the growth rate for the previously listed strains and the relative level of NaCl concentration. Comparing the YBR225W doubling times in 0mM and 750mM NaCl, there is a no effect on the growth rate. This effect has no influence on the knockout strain's (YBR225W) growth.
Caffeine Group 1
When adding caffeine to the YBR225W strain the growth rate had a moderate effect. The doubling time of the strains were as followed:
Doubling time of YBR225W with caffeine: 296 minutes
Doubling time of YBR225W without caffeine: 208 minutes
Doubling time of BY4735 with caffeine: -2649 minutes
Doubling time of BY4735 without caffeine: 187 minutes
The effect that the caffeine had on the YBR225W knockout strain increased the doubling time. The effect was it made the yeast grow at a slower rate.
References
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