Difference between revisions of "YMR090W"
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===Stressing with Hydroxyurea=== | ===Stressing with Hydroxyurea=== | ||
Revision as of 09:39, 6 May 2019
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| Systematic name | YMR090W |
| Gene name | |
| Aliases | |
| Feature type | ORF, Uncharacterized |
| Coordinates | Chr XIII:449245..449928 |
| Primary SGDID | S000004696 |
Description of YMR090W: Putative protein of unknown function with similarity to DTDP-glucose 4,6-dehydratases; GFP-fusion protein localizes to the cytoplasm; up-regulated in response to the fungicide mancozeb; not essential for viability[1][2][3]
Contents
Community Commentary
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UW-Stout/Sensitivity To Nitrogen Starvation
Knocking out the YMRO90W seems to have no effect on growth after incubating for 5 days on the Nitrogen omitted media.
Ultraviolet Sensitivity
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YPL068C is more sensitive than YMR090W given that there is less colonizes present when under the same stress.
DNA and RNA Details
Other DNA and RNA Details
Other Topic: expression
Specifically higher expression in nitrogen limited chemostat cultures versus nitrogen excess. [4] [5]
This gene is part of the UW-Stout Orphan Gene Project. Learn more here.
Growth Curve
In a BY4735 background, knocking out YMR090W seems to have a moderate effect on growth rate in log-phase. In this assay, the BY4735 strain's doubling time was 124 minutes, while the YMR090W knock-out strain's doubling time was 208 minutes.
G-418 Stress
In the BY4735 background, knocking out YMR090W seems to have a little effect on the growth rate. In the knock-out experiment, the BY4735 strain's doubling time was 149 minutes, whereas the YMR090W knock-out strain's doubling time was 242 minutes. The calibration experiment, the BY4735 strain's doubling time was 64 minutes, whereas the YMR090W knock-out strain's doubling time was 95 minutes.
Methanol Sensitivity
The wild type had a doubling time of 170 min, the YMR090w had a doubling time of 225 min. The doubling time increased for both after we added the methanol which means that this strain is sensitive.
Heat Shock
Stressing with Hydroxyurea
Hydroxyurea Protocol -The wild type strain had only a small difference in doubling time when stressed with Hydroxyurea;YMR090W showed about a 30 minute difference in doubling time compared to the wild type strain, indicating that this gene most likely plays a role in DNA replication.
Fermentation
Fermentation protocol link [2] - There appears to be no major difference in fermentation rate. The lower final ethanol percentage could be a result of evaporation. If this experiment were to be carried out again then it should be done in an air tight container.
References
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- ↑ Giaever G, et al. (2002) Functional profiling of the Saccharomyces cerevisiae genome. Nature 418(6896):387-91 SGD PMID 12140549
- ↑ Huh WK, et al. (2003) Global analysis of protein localization in budding yeast. Nature 425(6959):686-91 SGD PMID 14562095
- ↑ Santos PM, et al. (2009) Insights into yeast adaptive response to the agricultural fungicide mancozeb: a toxicoproteomics approach. Proteomics 9(3):657-70 SGD PMID 19137554
- ↑ Boer VM, et al. (2003) The genome-wide transcriptional responses of Saccharomyces cerevisiae grown on glucose in aerobic chemostat cultures limited for carbon, nitrogen, phosphorus, or sulfur. J Biol Chem 278(5):3265-74 SGD PMID 12414795
- ↑ submitted by Viktor Boer on 2003-07-25
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