Difference between revisions of "YCR100C"
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YCR100C is more sensitive than BY4735(wild) given that there is less colonizes present when under the same stress. | YCR100C is more sensitive than BY4735(wild) given that there is less colonizes present when under the same stress. | ||
| − | ===G-418 Stress=== | + | ===[[G-418 Stress]]=== |
Revision as of 16:44, 5 May 2019
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| Systematic name | YCR100C |
| Gene name | |
| Aliases | |
| Feature type | ORF, Uncharacterized |
| Coordinates | Chr III:302221..301271 |
| Primary SGDID | S000000697 |
Description of YCR100C: Putative protein of unknown function[1]
Contents
Community Commentary
About Community Commentary. Please share your knowledge!
This gene is part of the UW-Stout Orphan Gene Project. Learn more here.
UW-Stout/Sensitivity To Nitrogen Starvation
Knocking out the YCR100C seems to have no effect on growth after incubating for 5 days on the Nitrogen omitted media.
Growth Curve
In a BY4735 background, knocking out YCR100C seems to have a moderate effect on growth rate in log-phase. In this assay, the BY4735 strain's doubling time was 124 minutes, while the YCR100C knock-out strain's doubling time was 219 minutes.
Ultraviolet Sensitivity
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YCR100C is more sensitive than BY4735(wild) given that there is less colonizes present when under the same stress.
G-418 Stress
In the BY4735 background, knocking out the YCR100C strain seem to have a very small effect on a growth rate by slowing it down. In the knock-out experiment, the BY4735 strain's doubling time was 149 minutes, whereas the YCR100C knock-out strain's doubling time was 235 minutes. In the calibration experiment, the BY4735 strain's doubling time was 64 minutes, whereas the YCR100C knock-out strain's doubling time was 92 minutes.
Methanol Sensitivity
The wild type had a doubling time of 150 min, the YCR100c had a doubling time of 350 min. The methanol slowed the yeast down and that means this strain is more sensitive.
Stressing with Hydroxyurea
Hydroxyurea Protocol -The wild type strain had only a small difference in doubling time when stressed with Hydroxyurea;YCR100C showed about a 20 minute difference in doubling time when stressed with hydroxyurea, indicating that this gene most likely does not play a vital role in DNA replication.
Fermentation
Fermentation protocol link [2] - There appears to be no major difference in fermentation rate. The lower final ethanol percentage could be a result of evaporation. If this experiment were to be carried out again then it should be done in an air tight container.
References
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