Difference between revisions of "YFL064C"
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[[File:YFL064C_sensitivity_Methanol.png]] | [[File:YFL064C_sensitivity_Methanol.png]] | ||
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| + | ===Stressing with Hydroxyurea=== | ||
| + | [[UW-Stout/Hydroxyurea|Hydroxyurea Protocol]] | ||
| + | -The wild type strain had only a small difference in doubling time when stressed with Hydroxyurea;YFL064C had about a 10 minute difference of doubling time when stressed with hydroxyurea, indicating that this gene strain most likely does not play a vital role in DNA replication. | ||
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Revision as of 07:18, 2 May 2019
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| Systematic name | YFL064C |
| Gene name | |
| Aliases | |
| Feature type | ORF, Uncharacterized |
| Coordinates | Chr VI:3846..3322 |
| Primary SGDID | S000001830 |
Description of YFL064C: Putative protein of unknown function[1]
Contents
Community Commentary
About Community Commentary. Please share your knowledge!
This gene is part of the UW-Stout Orphan Gene Project. Learn more here.
UW-Stout/Sensitivity To Nitrogen Starvation
Knocking out the YFL064C seems to have no effect on growth after incubating for 5 days on the Nitrogen omitted media.
Ultraviolet Sensitivity
_uv_light.jpg)
YFL064C is more sensitive than BY4735(wild) given that there is less colonizes present when under the same stress.
Growth Curve
In a BY4735 background, knocking out YFL064C seems to have no effect on growth rate in log-phase. In this assay, the BY4735 strain's doubling time was 124 minutes, and the YFL064C knock-out strain's doubling time was also 124 minutes. However, the knock-out strain did reach a substantially higher OD600 before saturating.
Methanol Sensitivity
The wild type had a doubling time of 200 min, the YFL064c had a doubling time of 170 min. The methanol had little to no effect on these strains.
Stressing with Hydroxyurea
Hydroxyurea Protocol -The wild type strain had only a small difference in doubling time when stressed with Hydroxyurea;YFL064C had about a 10 minute difference of doubling time when stressed with hydroxyurea, indicating that this gene strain most likely does not play a vital role in DNA replication.
References
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