Difference between revisions of "YFL064C"
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+ | ===[[UW-Stout/Sensitivity To Nitrogen Starvation]]=== | ||
+ | [[File: YFL064C_growth plate.JPG|thumb|Growth of YFL064C on synthetic defined media (SDC).]] | ||
+ | [[File: YFL064C_starvation plate.JPG|thumb|Growth of YFL064C on omission media that lacks nitrogen.]] | ||
+ | [[File: YFL064C_revival plate.JPG|thumb|Growth of transferred BY4735 on Synthetic defined media.]] | ||
+ | Knocking out the YFL064C seems to have no effect on growth after incubating for 5 days on the Nitrogen omitted media. | ||
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===[[UW-Stout/Ultraviolet Light|Ultraviolet Sensitivity]]=== | ===[[UW-Stout/Ultraviolet Light|Ultraviolet Sensitivity]]=== |
Revision as of 06:36, 2 May 2019
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Systematic name | YFL064C |
Gene name | |
Aliases | |
Feature type | ORF, Uncharacterized |
Coordinates | Chr VI:3846..3322 |
Primary SGDID | S000001830 |
Description of YFL064C: Putative protein of unknown function[1]
Contents
Community Commentary
About Community Commentary. Please share your knowledge!
This gene is part of the UW-Stout Orphan Gene Project. Learn more here.
UW-Stout/Sensitivity To Nitrogen Starvation
Knocking out the YFL064C seems to have no effect on growth after incubating for 5 days on the Nitrogen omitted media.
Ultraviolet Sensitivity
YFL064C is more sensitive than BY4735(wild) given that there is less colonizes present when under the same stress.
Growth Curve
In a BY4735 background, knocking out YFL064C seems to have no effect on growth rate in log-phase. In this assay, the BY4735 strain's doubling time was 124 minutes, and the YFL064C knock-out strain's doubling time was also 124 minutes. However, the knock-out strain did reach a substantially higher OD600 before saturating.
Methanol Sensitivity
The wild type had a doubling time of 200 min, the YFL064c had a doubling time of 170 min. The methanol had little to no effect on these strains.
References
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