Difference between revisions of "YMR221C"

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===[[UW-Stout/Heat Shock FA21]]===
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[[Image: Heat Shock.jpeg|center|thumb|400px]]
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YMR221C (C)
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*When stressing this strain of yeast, there was not much of a growth difference. The growth rate slowed down once heat shocked.
  
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===[[UW-Stout/Nitrogen_Starvation_FA21|Nitrogen Starvation]]===
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[[Image:YMR221C_Nitrogen21.jpg]]
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*In the YMR221C gene, it seems that there is no significant difference between the two. If we are really taking a detailed look at it, it does seem that the plate without Ammonium is growing better. We conclude that the gene does not react with this strain.
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===[[UW-Stout/Bud Scars FA21]]===
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Cells grown with this gene (YMR221C) knocked out had an average of 1.36 bud scars per cell while the wild type (BY-RY4735) had 0.85 bud scars per cell. Of 119 YMR221C cells counted, 161 bud scars were seen. This indicated that the YMR221C gene slows reproduction rate and thus bud scarring in yeast cells.
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===[[UW-Stout/pH_FA21|pH]]===
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[[File:pH_knockout.jpg]]
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YMR221C
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*When this strain was exposed to a citric acid buffer with a pH of 2.6, there did not appear to be a significant change in the growth rate of the strain
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===[[UW-Stout/Ethanol FA21]]===
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[[File:Yeast ToutureV2.jpg|400px]]
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* The Yeast Strain of YMR221C was affected by the 18.5 % Ethanol but there was still a defined growth curve
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[[UW-Stout/Ethanol FA21]]
  
  
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Protocol: [[https://wiki.yeastgenome.org/index.php/UW-Stout/pH_FA21]]
  
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===[[UW-Stout/G418 FA21]]===
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[[File:YMR221C Vs BYR4735 G418.png|left|thumb|400px]]
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The YMR221C yeast strain doubling time without any stress was 172 minutes which is very similar to the wild type with 182 minutes. When both strains were stressed by G418 they YMR221C doubling time was 184 minutes and the wild type was 178 minutes. (These times are averaged between three trials)
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Protocol:[[https://wiki.yeastgenome.org/index.php/UW-Stout/G418_FA21]]
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===[[UW-Stout/Copper FA21]]===
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[[File:YMR2.jpg]]
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The average doubling time for YMR221C unstressed was 295 minutes, while stressed it was 294. From these observations we can conclude that while stressed with our .5 mM CuSO4 solution, the YMR221C strain grew approximately 1% faster. Although, the unstressed version had a higher overall size.
  
 
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==References==
 
==References==
 
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Latest revision as of 14:27, 21 December 2021

Share your knowledge...Edit this entry! <protect>

Systematic name YMR221C
Gene name
Aliases
Feature type ORF, Uncharacterized
Coordinates Chr XIII:715445..713931
Primary SGDID S000004834


Description of YMR221C: Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; physical interaction with Atg27p suggests a possible role in autophagy[1][2]




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Community Commentary

About Community Commentary. Please share your knowledge!

This gene is part of the UW-Stout Orphan Gene Project. Learn more here.

UW-Stout/Heat Shock FA21

Heat Shock.jpeg

YMR221C (C)

  • When stressing this strain of yeast, there was not much of a growth difference. The growth rate slowed down once heat shocked.

Nitrogen Starvation

YMR221C Nitrogen21.jpg

  • In the YMR221C gene, it seems that there is no significant difference between the two. If we are really taking a detailed look at it, it does seem that the plate without Ammonium is growing better. We conclude that the gene does not react with this strain.

UW-Stout/Bud Scars FA21

Cells grown with this gene (YMR221C) knocked out had an average of 1.36 bud scars per cell while the wild type (BY-RY4735) had 0.85 bud scars per cell. Of 119 YMR221C cells counted, 161 bud scars were seen. This indicated that the YMR221C gene slows reproduction rate and thus bud scarring in yeast cells.

pH

pH knockout.jpg

YMR221C

  • When this strain was exposed to a citric acid buffer with a pH of 2.6, there did not appear to be a significant change in the growth rate of the strain

UW-Stout/Ethanol FA21

Yeast ToutureV2.jpg

  • The Yeast Strain of YMR221C was affected by the 18.5 % Ethanol but there was still a defined growth curve

UW-Stout/Ethanol FA21



Protocol: [[1]]


UW-Stout/G418 FA21

YMR221C Vs BYR4735 G418.png

The YMR221C yeast strain doubling time without any stress was 172 minutes which is very similar to the wild type with 182 minutes. When both strains were stressed by G418 they YMR221C doubling time was 184 minutes and the wild type was 178 minutes. (These times are averaged between three trials) -->


Protocol:[[2]]








UW-Stout/Copper FA21

YMR2.jpg

The average doubling time for YMR221C unstressed was 295 minutes, while stressed it was 294. From these observations we can conclude that while stressed with our .5 mM CuSO4 solution, the YMR221C strain grew approximately 1% faster. Although, the unstressed version had a higher overall size.

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References

See Help:References on how to add references

  1. ↑ Reinders J, et al. (2006) Toward the complete yeast mitochondrial proteome: multidimensional separation techniques for mitochondrial proteomics. J Proteome Res 5(7):1543-54 SGD PMID 16823961
  2. ↑ Tarassov K, et al. (2008) An in vivo map of the yeast protein interactome. Science 320(5882):1465-70 SGD PMID 18467557

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