Difference between revisions of "YAL061W"
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| − | This graph shows the growth curve of ethanol tolerance in YAL061. | + | This graph shows the growth curve of ethanol tolerance in YAL061. The unstressed lines represent YAL061 that was not treated with ethanol. If you examine the graph you will notice that the strands that were treated with yeast grew more than the ones that weren't. The average doubling time for the stressed yeast was (answer). The average doubling time for the unstressed yeast was (answer). The results show that YAL061 in ethanol leads to an increase in growth. |
Revision as of 09:53, 12 December 2019
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| Systematic name | YAL061W |
| Gene name | BDH2 |
| Aliases | |
| Feature type | ORF, Uncharacterized |
| Coordinates | Chr I:33448..34701 |
| Primary SGDID | S000000057 |
Description of YAL061W: Putative medium-chain alcohol dehydrogenase with similarity to BDH1; transcription induced by constitutively active PDR1 and PDR3[1][2][3][4]
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Contents
Community Commentary
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This gene is part of the UW-Stout Orphan Gene Project. Learn more here.
Growth Curve
In a BY4735 background, knocking out YAL061W seems to have a moderate effect on growth rate in log-phase. In this assay, the BY4735 strain's doubling time was 361 minutes, while the YAL061 knock-out strain's doubling time was 469 minutes. (These doubling times are the means of three experiments.)
Caffeine and Yeast Cells
This graph shows the growth curve with and without caffeine. It has the knockout strain and wild type yeast cells. The caffeine enhanced the growth in both wild type and knockout strain. The average doubling time between the three experiments for the knockout strand was 777.27 minutes. The caffeine also had a positive effect on the growth curve for the wild type with the average doubling time being 1245.02 minutes. The results were concluded from the wild type and knockout strand was with this amount of caffeine it leads to an increase in growth.
The protocol can be found at
Caffeine
Ethanol Tolerance for YAL061
This graph shows the growth curve of ethanol tolerance in YAL061. The unstressed lines represent YAL061 that was not treated with ethanol. If you examine the graph you will notice that the strands that were treated with yeast grew more than the ones that weren't. The average doubling time for the stressed yeast was (answer). The average doubling time for the unstressed yeast was (answer). The results show that YAL061 in ethanol leads to an increase in growth.
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References
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- ↑ Devaux F, et al. (2002) Genome-wide studies on the nuclear PDR3-controlled response to mitochondrial dysfunction in yeast. FEBS Lett 515(1-3):25-8 SGD PMID 11943188
- ↑ Dickinson JR, et al. (2003) The catabolism of amino acids to long chain and complex alcohols in Saccharomyces cerevisiae. J Biol Chem 278(10):8028-34 SGD PMID 12499363
- ↑ Gonzalez E, et al. (2000) Characterization of a (2R,3R)-2,3-butanediol dehydrogenase as the Saccharomyces cerevisiae YAL060W gene product. Disruption and induction of the gene. J Biol Chem 275(46):35876-85 SGD PMID 10938079
- ↑ do Valle Matta MA, et al. (2001) Novel target genes of the yeast regulator Pdr1p: a contribution of the TPO1 gene in resistance to quinidine and other drugs. Gene 272(1-2):111-9 SGD PMID 11470516
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