Difference between revisions of "YFL064C"
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In a BY4735 background, knocking out YFL064C seems to have no effect on growth rate in log-phase. In this assay, the BY4735 strain's doubling time was 124 minutes, and the YFL064C knock-out strain's doubling time was also 124 minutes. However, the knock-out strain did reach a substantially higher OD600 before saturating. | In a BY4735 background, knocking out YFL064C seems to have no effect on growth rate in log-phase. In this assay, the BY4735 strain's doubling time was 124 minutes, and the YFL064C knock-out strain's doubling time was also 124 minutes. However, the knock-out strain did reach a substantially higher OD600 before saturating. | ||
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| + | ===G-418 Stress=== | ||
[[File:image007.png]] | [[File:image007.png]] | ||
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| + | In the BY4735 background, knocking out the YFL064C strain seem to have a different kind of effect, where the YFL064C growth was slowed down but grew faster than the BY4735. In the knock-out experiment, the BY4735 strain's doubling time was 149 minutes, whereas the YFL064C knock-out strain's doubling time was 142 minutes. In the calibration experiment, the BY4735 strain's doubling time was 64 minutes, whereas the YFL064C knock-out strain's doubling time was 100 minutes. | ||
===Methanol Sensitivity=== | ===Methanol Sensitivity=== | ||
Revision as of 18:56, 3 May 2019
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| Systematic name | YFL064C |
| Gene name | |
| Aliases | |
| Feature type | ORF, Uncharacterized |
| Coordinates | Chr VI:3846..3322 |
| Primary SGDID | S000001830 |
Description of YFL064C: Putative protein of unknown function[1]
Contents
Community Commentary
About Community Commentary. Please share your knowledge!
This gene is part of the UW-Stout Orphan Gene Project. Learn more here.
UW-Stout/Sensitivity To Nitrogen Starvation
Knocking out the YFL064C seems to have no effect on growth after incubating for 5 days on the Nitrogen omitted media.
Ultraviolet Sensitivity
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YFL064C is more sensitive than BY4735(wild) given that there is less colonizes present when under the same stress.
Growth Curve
In a BY4735 background, knocking out YFL064C seems to have no effect on growth rate in log-phase. In this assay, the BY4735 strain's doubling time was 124 minutes, and the YFL064C knock-out strain's doubling time was also 124 minutes. However, the knock-out strain did reach a substantially higher OD600 before saturating.
G-418 Stress
In the BY4735 background, knocking out the YFL064C strain seem to have a different kind of effect, where the YFL064C growth was slowed down but grew faster than the BY4735. In the knock-out experiment, the BY4735 strain's doubling time was 149 minutes, whereas the YFL064C knock-out strain's doubling time was 142 minutes. In the calibration experiment, the BY4735 strain's doubling time was 64 minutes, whereas the YFL064C knock-out strain's doubling time was 100 minutes.
Methanol Sensitivity
The wild type had a doubling time of 200 min, the YFL064c had a doubling time of 170 min. The methanol had little to no effect on these strains.
Stressing with Hydroxyurea
Hydroxyurea Protocol -The wild type strain had only a small difference in doubling time when stressed with Hydroxyurea;YFL064C had about a 10 minute difference of doubling time when stressed with hydroxyurea, indicating that this gene strain most likely does not play a vital role in DNA replication.
References
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