Difference between revisions of "YLR081W"

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=== Alleles, Strains, and Phenotypes ===
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[[Category:Topic:Alleles, Strains, and Phenotypes]]
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==== Multiple Knockout Strains ====
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[[Category:Topic:Alleles, Strains, and Phenotypes:Multiple Knockout Strains]]
  
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'''Strain Background''': CEN.PK2-1C<br>
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'''Genotype''': EBY4000 (hxt)<br>
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'''Together with''': HXT15, MPH2, HXT7, HXT6, HXT3, HXT13, HXT10, MAL11, HXT4, HXT1, HXT5, HXT12, HXT8, HXT9, HXT16, MPH3, HXT2, HXT14, HXT17, HXT11<br>
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'''Phenotype(s)''': Viable [[Category:Phenotype:Viable]]
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, Conditionally inviable when on glucose medium
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The complete hxt null strain (EBY4000) is unable to take up hexoses and to grow on them. It is well suited to express and characterize heterologous glucose transporters. <ref name='S000056317'>Wieczorke R, et al. (1999) Concurrent knock-out of at least 20 transporter genes is required to block uptake of hexoses in Saccharomyces cerevisiae. FEBS Lett 464(3):123-8 {{SGDpaper|S000056317}} PMID 10618490</ref> <ref name = 'CAset539-2003-03-17'>submitted by [http://db.yeastgenome.org/cgi-bin/colleague/colleagueSearch?id=539 Eckhard Boles] on 2003-03-17</ref>
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{{ShortCenteredHR}}
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=== Protein Details ===
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[[Category:Topic:Protein Details]]
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==== Protein Function/Process ====
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[[Category:Topic:Protein Details:Protein Function/Process]]
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The Gal2p galactose transporter is one of four Hxt transporters (Hxt4p, Hxt5p, Hxt7p, Gal2p) able to take up xylose <ref name='S000071422'>Hamacher T, et al. (2002) Characterization of the xylose-transporting properties of yeast hexose transporters and their influence on xylose utilization. Microbiology 148(Pt 9):2783-8 {{SGDpaper|S000071422}} PMID 12213924</ref> <ref name = 'CAset539-2003-03-17'>submitted by [http://db.yeastgenome.org/cgi-bin/colleague/colleagueSearch?id=539 Eckhard Boles] on 2003-03-17</ref>
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{{ShortCenteredHR}}
 
__TOC__
 
__TOC__
 
==Community Commentary==
 
==Community Commentary==
 
{{CommentaryHelp}}
 
{{CommentaryHelp}}
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=== Alleles, Strains, and Phenotypes ===
 +
[[Category:Topic:Alleles, Strains, and Phenotypes]]
 +
==== Multiple Knockout Strains ====
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[[Category:Topic:Alleles, Strains, and Phenotypes:Multiple Knockout Strains]]
 +
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'''Strain Background''': CEN.PK2-1C<br>
 +
'''Genotype''': EBY4000 (hxt)<br>
 +
'''Together with''': HXT15, MPH2, HXT7, HXT6, HXT3, HXT13, HXT10, MAL11, HXT4, HXT1, HXT5, HXT12, HXT8, HXT9, HXT16, MPH3, HXT2, HXT14, HXT17, HXT11<br>
 +
'''Phenotype(s)''': Viable [[Category:Phenotype:Viable]]
 +
, Conditionally inviable when on glucose medium
 +
 +
The complete hxt null strain (EBY4000) is unable to take up hexoses and to grow on them. It is well suited to express and characterize heterologous glucose transporters. <ref name='S000056317'>Wieczorke R, et al. (1999) Concurrent knock-out of at least 20 transporter genes is required to block uptake of hexoses in Saccharomyces cerevisiae. FEBS Lett 464(3):123-8 {{SGDpaper|S000056317}} PMID 10618490</ref> <ref name = 'CAset539-2003-03-17'>submitted by [http://db.yeastgenome.org/cgi-bin/colleague/colleagueSearch?id=539 Eckhard Boles] on 2003-03-17</ref>
 +
 +
{{ShortCenteredHR}}
 +
=== Protein Details ===
 +
[[Category:Topic:Protein Details]]
 +
==== Protein Function/Process ====
 +
[[Category:Topic:Protein Details:Protein Function/Process]]
 +
 +
The Gal2p galactose transporter is one of four Hxt transporters (Hxt4p, Hxt5p, Hxt7p, Gal2p) able to take up xylose <ref name='S000071422'>Hamacher T, et al. (2002) Characterization of the xylose-transporting properties of yeast hexose transporters and their influence on xylose utilization. Microbiology 148(Pt 9):2783-8 {{SGDpaper|S000071422}} PMID 12213924</ref> <ref name = 'CAset539-2003-03-17'>submitted by [http://db.yeastgenome.org/cgi-bin/colleague/colleagueSearch?id=539 Eckhard Boles] on 2003-03-17</ref>
  
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{{ShortCenteredHR}}
 
==References==
 
==References==
 
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Revision as of 17:05, 23 January 2007

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Systematic name YLR081W
Gene name GAL2
Aliases
Feature type ORF, Verified
Coordinates Chr XII:290213..291937
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Description of YLR081W: Galactose permease, required for utilization of galactose; also able to transport glucose[1][2][3]



Alleles, Strains, and Phenotypes

Multiple Knockout Strains

Strain Background: CEN.PK2-1C
Genotype: EBY4000 (hxt)
Together with: HXT15, MPH2, HXT7, HXT6, HXT3, HXT13, HXT10, MAL11, HXT4, HXT1, HXT5, HXT12, HXT8, HXT9, HXT16, MPH3, HXT2, HXT14, HXT17, HXT11
Phenotype(s): Viable , Conditionally inviable when on glucose medium

The complete hxt null strain (EBY4000) is unable to take up hexoses and to grow on them. It is well suited to express and characterize heterologous glucose transporters. [4] [5]


Protein Details

Protein Function/Process

The Gal2p galactose transporter is one of four Hxt transporters (Hxt4p, Hxt5p, Hxt7p, Gal2p) able to take up xylose [6] [5]


Community Commentary

About Community Commentary. Please share your knowledge!

Alleles, Strains, and Phenotypes

Multiple Knockout Strains

Strain Background: CEN.PK2-1C
Genotype: EBY4000 (hxt)
Together with: HXT15, MPH2, HXT7, HXT6, HXT3, HXT13, HXT10, MAL11, HXT4, HXT1, HXT5, HXT12, HXT8, HXT9, HXT16, MPH3, HXT2, HXT14, HXT17, HXT11
Phenotype(s): Viable , Conditionally inviable when on glucose medium

The complete hxt null strain (EBY4000) is unable to take up hexoses and to grow on them. It is well suited to express and characterize heterologous glucose transporters. [4] [5]


Protein Details

Protein Function/Process

The Gal2p galactose transporter is one of four Hxt transporters (Hxt4p, Hxt5p, Hxt7p, Gal2p) able to take up xylose [6] [5]


References

See Help:References on how to add references

  1. Maier A, et al. (2002) Characterisation of glucose transport in Saccharomyces cerevisiae with plasma membrane vesicles (countertransport) and intact cells (initial uptake) with single Hxt1, Hxt2, Hxt3, Hxt4, Hxt6, Hxt7 or Gal2 transporters. FEMS Yeast Res 2(4):539-50 SGD PMID 12702270
  2. Kasahara T and Kasahara M (2000) Three aromatic amino acid residues critical for galactose transport in yeast Gal2 transporter. J Biol Chem 275(6):4422-8 SGD PMID 10660614
  3. Rodriguez C and Flores C (2000) Mutations in GAL2 or GAL4 alleviate catabolite repression produced by galactose in Saccharomyces cerevisiae. Enzyme Microb Technol 26(9-10):748-755 SGD PMID 10862881
  4. 4.0 4.1 Wieczorke R, et al. (1999) Concurrent knock-out of at least 20 transporter genes is required to block uptake of hexoses in Saccharomyces cerevisiae. FEBS Lett 464(3):123-8 SGD PMID 10618490
  5. 5.0 5.1 5.2 5.3 submitted by Eckhard Boles on 2003-03-17
  6. 6.0 6.1 Hamacher T, et al. (2002) Characterization of the xylose-transporting properties of yeast hexose transporters and their influence on xylose utilization. Microbiology 148(Pt 9):2783-8 SGD PMID 12213924

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