Difference between revisions of "YJL219W"

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=== Alleles, Strains, and Phenotypes ===
 
[[Category:Topic:Alleles, Strains, and Phenotypes]]
 
==== Multiple Knockout Strains ====
 
[[Category:Topic:Alleles, Strains, and Phenotypes:Multiple Knockout Strains]]
 
 
'''Strain Background''': CEN.PK2-1C<br>
 
'''Genotype''': EBY4000 (hxt)<br>
 
'''Together with''': HXT15, MPH2, HXT7, HXT6, HXT3, HXT13, HXT10, MAL11, HXT4, HXT1, HXT5, HXT12, HXT8, HXT16, MPH3, GAL2, HXT2, HXT14, HXT17, HXT11<br>
 
'''Phenotype(s)''': Viable [[Category:Phenotype:Viable]]
 
, Conditionally inviable when on glucose medium
 
 
The complete hxt null strain (EBY4000) is unable to take up hexoses and to grow on them. It is well suited to express and characterize heterologous glucose transporters. <ref name='S000056317'>Wieczorke R, et al. (1999) Concurrent knock-out of at least 20 transporter genes is required to block uptake of hexoses in Saccharomyces cerevisiae. FEBS Lett 464(3):123-8 {{SGDpaper|S000056317}} PMID 10618490</ref> <ref name = 'CAset539-2003-03-17'>submitted by [http://db.yeastgenome.org/cgi-bin/colleague/colleagueSearch?id=539 Eckhard Boles] on 2003-03-17</ref>
 
 
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==Community Commentary==
 
==Community Commentary==

Revision as of 11:51, 24 January 2007

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Systematic name YJL219W
Gene name HXT9
Aliases
Feature type ORF, Verified
Coordinates Chr X:19497..21200
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Description of YJL219W: Putative hexose transporter that is nearly identical to Hxt11p, has similarity to major facilitator superfamily (MFS) transporters, expression of HXT9 is regulated by transcription factors Pdr1p and Pdr3p[1][2]



Community Commentary

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Alleles, Strains, and Phenotypes

Multiple Knockout Strains

Strain Background: CEN.PK2-1C
Genotype: EBY4000 (hxt)
Together with: HXT15, MPH2, HXT7, HXT6, HXT3, HXT13, HXT10, MAL11, HXT4, HXT1, HXT5, HXT12, HXT8, HXT16, MPH3, GAL2, HXT2, HXT14, HXT17, HXT11
Phenotype(s): Viable , Conditionally inviable when on glucose medium

The complete hxt null strain (EBY4000) is unable to take up hexoses and to grow on them. It is well suited to express and characterize heterologous glucose transporters. [3] [4]


References

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  1. Nourani A, et al. (1997) Multiple-drug-resistance phenomenon in the yeast Saccharomyces cerevisiae: involvement of two hexose transporters. Mol Cell Biol 17(9):5453-60 SGD PMID 9271421
  2. Ozcan S and Johnston M (1999) Function and regulation of yeast hexose transporters. Microbiol Mol Biol Rev 63(3):554-69 SGD PMID 10477308
  3. Wieczorke R, et al. (1999) Concurrent knock-out of at least 20 transporter genes is required to block uptake of hexoses in Saccharomyces cerevisiae. FEBS Lett 464(3):123-8 SGD PMID 10618490
  4. submitted by Eckhard Boles on 2003-03-17

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