Difference between revisions of "YGR198W"

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GFP-tag is more effective at the n-terminal end. Adding an epitope tag at the C-terminus makes the strain somewhat sick and lowers PI4P levels, however, it seems to localize correctly.
  
  
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==References==
 
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Revision as of 12:05, 17 August 2007

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Systematic name YGR198W
Gene name YPP1
Aliases
Feature type ORF, Verified
Coordinates Chr VII:894698..897151
Primary SGDID S000003430


Description of YGR198W: Cargo-transport protein involved in endocytosis; may interact with ribosomes, based on co-purification experiments; GFP-fusion protein localizes to the cytoplasm; YGR198W is an essential gene[1][2][3][4]




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Community Commentary

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GFP-tag is more effective at the n-terminal end. Adding an epitope tag at the C-terminus makes the strain somewhat sick and lowers PI4P levels, however, it seems to localize correctly.



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References

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  1. Flower T, et al. (2006) The Yeast Endocytosis Gene YGR198w (YPP1) Suppresses A30P Alpha-Synuclein Toxicity Nat Cell Biol () SGD PMID
  2. Fleischer TC, et al. (2006) Systematic identification and functional screens of uncharacterized proteins associated with eukaryotic ribosomal complexes. Genes Dev 20(10):1294-307 SGD PMID 16702403
  3. Huh WK, et al. (2003) Global analysis of protein localization in budding yeast. Nature 425(6959):686-91 SGD PMID 14562095
  4. Rodriguez-Pena JM, et al. (1998) The deletion of six ORFs of unknown function from Saccharomyces cerevisiae chromosome VII reveals two essential genes: YGR195w and YGR198w. Yeast 14(9):853-60 SGD PMID 9818723

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