Reagents

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*[[Antibodies]] made to ''S. cerevisiae'' proteins
 
*[[Antibodies]] made to ''S. cerevisiae'' proteins
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*Custom Oligos and Primers - Several companies provide custom oligo synthesis, including but not limited to, those listed here:
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**[http://www.geneworks.com.au/Content.aspx?p=84 GeneWorks]
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**[http://www.idtdna.com/catalog/catalog.aspx?adwords=oligo&gclid=CMT0tsS9_pMCFRwvagodWhd3WA Integrated DNA Technologies]
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**[http://www.invitrogen.com.cn/content.cfm?pageid=9714 Invitrogen]
  
 
*[http://www.openbiosystems.com/GeneExpression/Yeast/ORF/ MORF collection] is a moveable ORF library which enables protein expression and purification for more than 4900 <i>S. cerevisiae</i> genes, as described in [http://db.yeastgenome.org/cgi-bin/reference/reference.pl?dbid=S000113921 Gelperin DM, et al.  (2005)].
 
*[http://www.openbiosystems.com/GeneExpression/Yeast/ORF/ MORF collection] is a moveable ORF library which enables protein expression and purification for more than 4900 <i>S. cerevisiae</i> genes, as described in [http://db.yeastgenome.org/cgi-bin/reference/reference.pl?dbid=S000113921 Gelperin DM, et al.  (2005)].

Revision as of 17:22, 18 June 2008

  • pAG Vectors is a collection of 288 yeast Gateway expression vectors. These "Advanced Gateway" vectors are modifications of the pRS series plasmids and allow for inducible or constitutive expression, as well as fusion to a number of protein tags and fluorescent proteins. We have made all of these vectors available through the non-profit plasmid repository Addgene. Clones were created by the Lindquist Lab at the Whitehead Institute. "A suite of Gateway vectors for high-throughput genetic analysis in Saccharomyces cerevisiae". Simon Alberti, Aaron D. Gitler, and Susan Lindquist." 2007. Yeast. In Press.
  • Tritech Research maintains a list of links to S. cerevisiae information and protocols, and sells reagents and equipment for S. cerevisiae research.
  • VectorDB, collected sequences of cloning vectors for use in a variety of organisms
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