Difference between revisions of "YLR426W"
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==Community Commentary== | ==Community Commentary== | ||
{{CommentaryHelp}} | {{CommentaryHelp}} | ||
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| + | {{UW-Stout}} | ||
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| + | ===[[UW-Stout/Growth_Curve_SP21|Growth Curve]]=== | ||
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| + | [[Image:YLR426W-water.png]] | ||
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| + | In a BY4735 background, knocking out YLR426W seems to have a substantial effect on the strain's growth in complete media. In this assay, the BY4735 strain's doubling time was 162 minutes, while the YLR426W knock-out strain's doubling time was 660 minutes. (These doubling times are the means of three experiments.) | ||
Revision as of 08:27, 22 April 2021
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| Systematic name | YLR426W |
| Gene name | TDA5 |
| Aliases | |
| Feature type | ORF, Uncharacterized |
| Coordinates | Chr XII:987062..988113 |
| Primary SGDID | S000004418 |
Description of YLR426W: Putative protein of unknown function; detected in highly purified mitochondria in high-throughput studies; proposed to be involved in resistance to mechlorethamine and streptozotocin; null mutant sensitive to expression of top1-T722A allele[1][2][3][4]
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Community Commentary
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This gene is part of the UW-Stout Orphan Gene Project. Learn more here.
Growth Curve
In a BY4735 background, knocking out YLR426W seems to have a substantial effect on the strain's growth in complete media. In this assay, the BY4735 strain's doubling time was 162 minutes, while the YLR426W knock-out strain's doubling time was 660 minutes. (These doubling times are the means of three experiments.)
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References
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- ↑ Lee W, et al. (2005) Genome-wide requirements for resistance to functionally distinct DNA-damaging agents. PLoS Genet 1(2):e24 SGD PMID 16121259
- ↑ Reid RJ, et al. (2011) Selective ploidy ablation, a high-throughput plasmid transfer protocol, identifies new genes affecting topoisomerase I-induced DNA damage. Genome Res 21(3):477-86 SGD PMID 21173034
- ↑ Reinders J, et al. (2006) Toward the complete yeast mitochondrial proteome: multidimensional separation techniques for mitochondrial proteomics. J Proteome Res 5(7):1543-54 SGD PMID 16823961
- ↑ Sickmann A, et al. (2003) The proteome of Saccharomyces cerevisiae mitochondria. Proc Natl Acad Sci U S A 100(23):13207-12 SGD PMID 14576278
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