Difference between revisions of "YIL170W"

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=== Alleles, Strains, and Phenotypes ===
 
[[Category:Topic:Alleles, Strains, and Phenotypes]]
 
==== Multiple Knockout Strains ====
 
[[Category:Topic:Alleles, Strains, and Phenotypes:Multiple Knockout Strains]]
 
 
'''Strain Background''': CEN.PK2-1C<br>
 
'''Genotype''': EBY4000 (hxt)<br>
 
'''Together with''': HXT15, MPH2, HXT7, HXT6, HXT3, HXT13, HXT10, MAL11, HXT4, HXT1, HXT5, HXT8, HXT9, HXT16, MPH3, GAL2, HXT2, HXT14, HXT17, HXT11<br>
 
'''Phenotype(s)''': Viable [[Category:Phenotype:Viable]]
 
, Conditionally inviable when on glucose medium
 
 
The complete hxt null strain (EBY4000) is unable to take up hexoses and to grow on them. It is well suited to express and characterize heterologous glucose transporters. <ref name='S000056317'>Wieczorke R, et al. (1999) Concurrent knock-out of at least 20 transporter genes is required to block uptake of hexoses in Saccharomyces cerevisiae. FEBS Lett 464(3):123-8 {{SGDpaper|S000056317}} PMID 10618490</ref> <ref name = 'CAset539-2003-03-17'>submitted by [http://db.yeastgenome.org/cgi-bin/colleague/colleagueSearch?id=539 Eckhard Boles] on 2003-03-17</ref>
 
 
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==Community Commentary==
 
==Community Commentary==

Revision as of 11:51, 24 January 2007

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Systematic name YIL170W
Gene name HXT12
Aliases
Feature type pseudogene
Coordinates Chr IX:19847..21220
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Description of YIL170W: Possible pseudogene in strain S288C; YIL170W/HXT12 and the adjacent ORF, YIL171W, together encode a non-functional member of the hexose transporter family[1][2][3][4]



Community Commentary

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Alleles, Strains, and Phenotypes

Multiple Knockout Strains

Strain Background: CEN.PK2-1C
Genotype: EBY4000 (hxt)
Together with: HXT15, MPH2, HXT7, HXT6, HXT3, HXT13, HXT10, MAL11, HXT4, HXT1, HXT5, HXT8, HXT9, HXT16, MPH3, GAL2, HXT2, HXT14, HXT17, HXT11
Phenotype(s): Viable , Conditionally inviable when on glucose medium

The complete hxt null strain (EBY4000) is unable to take up hexoses and to grow on them. It is well suited to express and characterize heterologous glucose transporters. [4] [5]


References

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  1. Kruckeberg AL (1996) The hexose transporter family of Saccharomyces cerevisiae. Arch Microbiol 166(5):283-92 SGD PMID 8929273
  2. Andre B (1995) An overview of membrane transport proteins in Saccharomyces cerevisiae. Yeast 11(16):1575-611 SGD PMID 8720066
  3. Nelissen B, et al. (1995) Phylogenetic classification of the major superfamily of membrane transport facilitators, as deduced from yeast genome sequencing. FEBS Lett 377(2):232-6 SGD PMID 8543057
  4. 4.0 4.1 Wieczorke R, et al. (1999) Concurrent knock-out of at least 20 transporter genes is required to block uptake of hexoses in Saccharomyces cerevisiae. FEBS Lett 464(3):123-8 SGD PMID 10618490 Cite error: Invalid <ref> tag; name "S000056317" defined multiple times with different content
  5. submitted by Eckhard Boles on 2003-03-17

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