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| <!-- PLEASE DO NOT EDIT HERE. USE THE SECTION EDIT LINKS ON THE RIGHT MARGIN--> | | <!-- PLEASE DO NOT EDIT HERE. USE THE SECTION EDIT LINKS ON THE RIGHT MARGIN--> |
| {{PageTop}} | | {{PageTop}} |
| + | <protect> |
| {|{{Prettytable}} align = 'right' width = '200px' | | {|{{Prettytable}} align = 'right' width = '200px' |
| |- | | |- |
− | |valign="top" nowrap bgcolor="{{SGDblue}}"| '''Systematic name''' || [http://db.yeastgenome.org/cgi-bin/locus.pl?locus=YER027C YER027C] | + | |valign="top" nowrap bgcolor="{{SGDblue}}"| '''Systematic name''' || [http://www.yeastgenome.org/cgi-bin/locus.pl?dbid=S000000829 YER027C] |
| |- | | |- |
| |valign="top" nowrap bgcolor="{{SGDblue}}"| '''Gene name''' ||''GAL83 '' | | |valign="top" nowrap bgcolor="{{SGDblue}}"| '''Gene name''' ||''GAL83 '' |
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| |- | | |- |
| |valign="top" nowrap bgcolor="{{SGDblue}}"| '''Coordinates''' | | |valign="top" nowrap bgcolor="{{SGDblue}}"| '''Coordinates''' |
− | |nowrap| Chr V:210231..208978 | + | |nowrap| Chr V:210232..208979 |
| |- | | |- |
− | |colspan = '3'|{{Don'tEditThisBox}} | + | |valign="top" nowrap bgcolor="{{SGDblue}}"| '''Primary SGDID''' || S000000829 |
| |} | | |} |
| <br> | | <br> |
− | '''Description of {{PAGENAME}}:''' One of three possible beta-subunits of the Snf1 kinase complex, allows nuclear localization of the Snf1 kinase complex in the presence of a nonfermentable carbon source; contains glycogen-binding domain<ref name='S000074696'>Wiatrowski HA, et al. (2004) Mutations in the gal83 glycogen-binding domain activate the snf1/gal83 kinase pathway by a glycogen-independent mechanism. Mol Cell Biol 24(1):352-61 {{SGDpaper|S000074696}} PMID 14673168</ref><ref name='S000073587'>Schuller HJ (2003) Transcriptional control of nonfermentative metabolism in the yeast Saccharomyces cerevisiae. Curr Genet 43(3):139-60 | + | '''Description of YER027C:''' One of three possible beta-subunits of the Snf1 kinase complex, allows nuclear localization of the Snf1 kinase complex in the presence of a nonfermentable carbon source; contains glycogen-binding domain<ref name='S000073587'>Schuller HJ (2003) Transcriptional control of nonfermentative metabolism in the yeast Saccharomyces cerevisiae. Curr Genet 43(3):139-60 {{SGDpaper|S000073587}} PMID 12715202</ref><ref name='S000074696'>Wiatrowski HA, et al. (2004) Mutations in the gal83 glycogen-binding domain activate the snf1/gal83 kinase pathway by a glycogen-independent mechanism. Mol Cell Biol 24(1):352-61 |
− | {{SGDpaper|S000073587}} PMID 12715202</ref>
| + | {{SGDpaper|S000074696}} PMID 14673168</ref> |
| <br> | | <br> |
| <br> | | <br> |
| <br> | | <br> |
| <br> | | <br> |
| + | <br> |
| + | </protect> |
| + | __TOC__ |
| + | ==Community Commentary== |
| + | {{CommentaryHelp}} |
| === Alleles, Strains, and Phenotypes === | | === Alleles, Strains, and Phenotypes === |
| [[Category:Topic:Alleles, Strains, and Phenotypes]] | | [[Category:Topic:Alleles, Strains, and Phenotypes]] |
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| {{ShortCenteredHR}} | | {{ShortCenteredHR}} |
− | __TOC__
| |
− | ==Community Commentary==
| |
− | {{CommentaryHelp}}
| |
− | === Alleles, Strains, and Phenotypes ===
| |
− | [[Category:Topic:Alleles, Strains, and Phenotypes]]
| |
− | ==== Complete Deletion ====
| |
− | [[Category:Topic:Alleles, Strains, and Phenotypes:Complete Deletion]]
| |
− | '''Allele''': gal83delta<br>
| |
− |
| |
− | '''Strain Background''': Sigma1278b (Sigma2000 series)<br>
| |
− | '''Genotype''': MCY4567<br>
| |
− | '''Together with''': NRG2, NRG1, SIP1, SIP2<br>
| |
− | '''Phenotype(s)''': Loss of function (Null) [[Category:Phenotype:Loss of function (Null)]]
| |
− |
| |
− | gal83 mutants are defective in adherance to plastic in response to limiting glucose. <ref name='S000072732'>Vyas VK, et al. (2003) Snf1 kinases with different beta-subunit isoforms play distinct roles in regulating haploid invasive growth. Mol Cell Biol 23(4):1341-8 {{SGDpaper|S000072732}} PMID 12556493</ref> <ref name = 'CAset6176-2003-03-27'>submitted by [http://db.yeastgenome.org/cgi-bin/colleague/colleagueSearch?id=6176 Valmik K. Vyas] on 2003-03-27</ref>
| |
− |
| |
− | {{ShortCenteredHR}}
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− | '''Allele''': gal83delta<br>
| |
− |
| |
− | '''Strain Background''': Sigma1278b<br>
| |
− | '''Genotype''': MCY4567<br>
| |
− | '''Together with''': NRG2, NRG1, SIP1, SIP2<br>
| |
− | '''Phenotype(s)''': Loss of function (Null) [[Category:Phenotype:Loss of function (Null)]]
| |
− |
| |
− | gal83 mutants are defective in invasive growth in response to limiting glucose. <ref name='S000072732'>Vyas VK, et al. (2003) Snf1 kinases with different beta-subunit isoforms play distinct roles in regulating haploid invasive growth. Mol Cell Biol 23(4):1341-8 {{SGDpaper|S000072732}} PMID 12556493</ref> <ref name = 'CAset6176-2003-03-27'>submitted by [http://db.yeastgenome.org/cgi-bin/colleague/colleagueSearch?id=6176 Valmik K. Vyas] on 2003-03-27</ref>
| |
− |
| |
− | {{ShortCenteredHR}}
| |
− | '''Allele''': gal83delta<br>
| |
− |
| |
− | '''Strain Background''': Sigma1278b (Sigma2000 series)<br>
| |
− | '''Genotype''': MCY4567<br>
| |
− | '''Together with''': NRG2, NRG1, SIP1, SIP2<br>
| |
− | '''Phenotype(s)''': Loss of function (Null) [[Category:Phenotype:Loss of function (Null)]]
| |
− |
| |
− | gal83 mutants are defective in adherance to plastic in response to limiting glucose. <ref name='S000072732'>Vyas VK, et al. (2003) Snf1 kinases with different beta-subunit isoforms play distinct roles in regulating haploid invasive growth. Mol Cell Biol 23(4):1341-8 {{SGDpaper|S000072732}} PMID 12556493</ref> <ref name = 'CAset6176-2003-03-27'>submitted by [http://db.yeastgenome.org/cgi-bin/colleague/colleagueSearch?id=6176 Valmik K. Vyas] on 2003-03-27</ref>
| |
− |
| |
− | {{ShortCenteredHR}}
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− | '''Allele''': gal83 delta<br>
| |
− |
| |
− | '''Strain Background''': Sigma1278b (Sigma2000 series)<br>
| |
− | '''Genotype''': MCY 4567<br>
| |
− | '''Together with''': NRG2, NRG1, SIP1, SIP2<br>
| |
− | '''Phenotype(s)''': Loss of function (Null) [[Category:Phenotype:Loss of function (Null)]]
| |
| | | |
− | gal83 mutants exhibit wild-type filamentation in response to glucose depletion. <ref name='S000072732'>Vyas VK, et al. (2003) Snf1 kinases with different beta-subunit isoforms play distinct roles in regulating haploid invasive growth. Mol Cell Biol 23(4):1341-8 {{SGDpaper|S000072732}} PMID 12556493</ref> <ref name = 'CAset6176-2003-03-27'>submitted by [http://db.yeastgenome.org/cgi-bin/colleague/colleagueSearch?id=6176 Valmik K. Vyas] on 2003-03-27</ref>
| |
| | | |
− | {{ShortCenteredHR}}
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− | '''Allele''': gal83delta<br>
| |
| | | |
− | '''Strain Background''': Sigma1278b<br>
| + | <!-- PLEASE ADD Community Commentary ABOVE THIS MESSAGE. See below for an example of community annotation --> |
− | '''Genotype''': MCY4567<br>
| + | <!-- |
− | '''Together with''': NRG2, NRG1, SIP1, SIP2<br>
| + | Specifically higher expression in carbon limited chemostat cultures versus carbon excess. |
− | '''Phenotype(s)''': Loss of function (Null) [[Category:Phenotype:Loss of function (Null)]]
| + | <ref>Boer VM, et al. (2003) The genome-wide transcriptional responses of Saccharomyces cerevisiae grown on glucose in aerobic chemostat cultures limited for carbon, nitrogen, phosphorus, or sulfur. |
| + | J Biol Chem 278(5):3265-74</ref> |
| + | --> |
| | | |
− | gal83 mutants are defective in invasive growth in response to limiting glucose. <ref name='S000072732'>Vyas VK, et al. (2003) Snf1 kinases with different beta-subunit isoforms play distinct roles in regulating haploid invasive growth. Mol Cell Biol 23(4):1341-8 {{SGDpaper|S000072732}} PMID 12556493</ref> <ref name = 'CAset6176-2003-03-27'>submitted by [http://db.yeastgenome.org/cgi-bin/colleague/colleagueSearch?id=6176 Valmik K. Vyas] on 2003-03-27</ref>
| |
| | | |
− | {{ShortCenteredHR}}
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− | '''Allele''': gal83 delta<br>
| |
| | | |
− | '''Strain Background''': Sigma1278b (Sigma2000 series)<br>
| + | <protect> |
− | '''Genotype''': MCY 4567<br>
| |
− | '''Together with''': NRG2, NRG1, SIP1, SIP2<br>
| |
− | '''Phenotype(s)''': Loss of function (Null) [[Category:Phenotype:Loss of function (Null)]]
| |
− | | |
− | gal83 mutants exhibit wild-type filamentation in response to glucose depletion. <ref name='S000072732'>Vyas VK, et al. (2003) Snf1 kinases with different beta-subunit isoforms play distinct roles in regulating haploid invasive growth. Mol Cell Biol 23(4):1341-8 {{SGDpaper|S000072732}} PMID 12556493</ref> <ref name = 'CAset6176-2003-03-27'>submitted by [http://db.yeastgenome.org/cgi-bin/colleague/colleagueSearch?id=6176 Valmik K. Vyas] on 2003-03-27</ref>
| |
− | | |
− | {{ShortCenteredHR}}
| |
− | ==== Multiple Knockout Strains ====
| |
− | [[Category:Topic:Alleles, Strains, and Phenotypes:Multiple Knockout Strains]]
| |
− | | |
− | '''Strain Background''': Sigma1278b (Sigma 2000 series)<br>
| |
− | '''Genotype''': MCY4708<br>
| |
− | '''Together with''': NRG2, NRG1, SIP1, SIP2<br>
| |
− | '''Phenotype(s)''': Loss of function (Null) [[Category:Phenotype:Loss of function (Null)]]
| |
− | | |
− | Deletion of nrg2 partially suppresses gal83's defect in invasive growth, plastic adherance, and STA2-lacZ expression. <ref name='S000072732'>Vyas VK, et al. (2003) Snf1 kinases with different beta-subunit isoforms play distinct roles in regulating haploid invasive growth. Mol Cell Biol 23(4):1341-8 {{SGDpaper|S000072732}} PMID 12556493</ref> <ref name = 'CAset6176-2003-03-27'>submitted by [http://db.yeastgenome.org/cgi-bin/colleague/colleagueSearch?id=6176 Valmik K. Vyas] on 2003-03-27</ref>
| |
− | | |
− | {{ShortCenteredHR}}
| |
− | | |
− | '''Strain Background''': Sigma1278b (Sigma 2000 series)<br>
| |
− | '''Genotype''': MCY4716<br>
| |
− | '''Together with''': NRG2, NRG1, SIP1, SIP2<br>
| |
− | '''Phenotype(s)''': Loss of function (Null) [[Category:Phenotype:Loss of function (Null)]]
| |
− | | |
− | Deletion of nrg1 partially suppresses gal83's defect in invasive growth, plastic adherance, and STA2-lacZ expression. <ref name='S000072732'>Vyas VK, et al. (2003) Snf1 kinases with different beta-subunit isoforms play distinct roles in regulating haploid invasive growth. Mol Cell Biol 23(4):1341-8 {{SGDpaper|S000072732}} PMID 12556493</ref> <ref name = 'CAset6176-2003-03-27'>submitted by [http://db.yeastgenome.org/cgi-bin/colleague/colleagueSearch?id=6176 Valmik K. Vyas] on 2003-03-27</ref>
| |
− | | |
− | {{ShortCenteredHR}}
| |
− | | |
− | '''Strain Background''': Sigma1278b (Sigma2000 series)<br>
| |
− | '''Genotype''': MCY 4597<br>
| |
− | '''Together with''': NRG2, NRG1, SIP1, SIP2<br>
| |
− | '''Phenotype(s)''': Loss of function (Null) [[Category:Phenotype:Loss of function (Null)]]
| |
− | | |
− | sip2 gal83 double mutants are more defective for invasive growth in response to limiting glucose than gal83 single mutants. <ref name='S000072732'>Vyas VK, et al. (2003) Snf1 kinases with different beta-subunit isoforms play distinct roles in regulating haploid invasive growth. Mol Cell Biol 23(4):1341-8 {{SGDpaper|S000072732}} PMID 12556493</ref> <ref name = 'CAset6176-2003-03-27'>submitted by [http://db.yeastgenome.org/cgi-bin/colleague/colleagueSearch?id=6176 Valmik K. Vyas] on 2003-03-27</ref>
| |
− | | |
− | {{ShortCenteredHR}}
| |
− | | |
− | '''Strain Background''': Sigma1278b (Sigma2000 series)<br>
| |
− | '''Genotype''': MCY4581<br>
| |
− | '''Together with''': NRG2, NRG1, SIP1, SIP2<br>
| |
− | '''Phenotype(s)''': Loss of function (Null) [[Category:Phenotype:Loss of function (Null)]]
| |
− | | |
− | Deletion of SIP1 suppresses gal83's defect in invasive growth in response to glucose limitation. <ref name='S000072732'>Vyas VK, et al. (2003) Snf1 kinases with different beta-subunit isoforms play distinct roles in regulating haploid invasive growth. Mol Cell Biol 23(4):1341-8 {{SGDpaper|S000072732}} PMID 12556493</ref> <ref name = 'CAset6176-2003-03-27'>submitted by [http://db.yeastgenome.org/cgi-bin/colleague/colleagueSearch?id=6176 Valmik K. Vyas] on 2003-03-27</ref>
| |
− | | |
− | {{ShortCenteredHR}}
| |
− | | |
− | '''Strain Background''': Sigma1278b (Sigma 2000 series)<br>
| |
− | '''Genotype''': MCY4712<br>
| |
− | '''Together with''': NRG2, NRG1, SIP1, SIP2<br>
| |
− | '''Phenotype(s)''': Loss of function (Null) [[Category:Phenotype:Loss of function (Null)]]
| |
− | | |
− | Deletion of nrg1 and nrg2 suppresses gal83's defect in invasive growth, plastic adherance, and STA2-lacZ expression, better than deletion of either nrg alone. <ref name='S000072732'>Vyas VK, et al. (2003) Snf1 kinases with different beta-subunit isoforms play distinct roles in regulating haploid invasive growth. Mol Cell Biol 23(4):1341-8 {{SGDpaper|S000072732}} PMID 12556493</ref> <ref name = 'CAset6176-2003-03-27'>submitted by [http://db.yeastgenome.org/cgi-bin/colleague/colleagueSearch?id=6176 Valmik K. Vyas] on 2003-03-27</ref>
| |
− | | |
− | {{ShortCenteredHR}}
| |
− | | |
− | '''Strain Background''': Sigma1278b (Sigma 2000 series)<br>
| |
− | '''Genotype''': MCY4597<br>
| |
− | '''Together with''': NRG2, NRG1, SIP1, SIP2<br>
| |
− | '''Phenotype(s)''': Loss of function (Null) [[Category:Phenotype:Loss of function (Null)]]
| |
− | | |
− | sip2 gal83 double mutants are defective for filamentation in response to glucose depletion. <ref name='S000072732'>Vyas VK, et al. (2003) Snf1 kinases with different beta-subunit isoforms play distinct roles in regulating haploid invasive growth. Mol Cell Biol 23(4):1341-8 {{SGDpaper|S000072732}} PMID 12556493</ref> <ref name = 'CAset6176-2003-03-27'>submitted by [http://db.yeastgenome.org/cgi-bin/colleague/colleagueSearch?id=6176 Valmik K. Vyas] on 2003-03-27</ref>
| |
− | | |
− | {{ShortCenteredHR}}
| |
− | | |
− | '''Strain Background''': Sigma1278b (Sigma2000 series)<br>
| |
− | '''Genotype''': MCY4581<br>
| |
− | '''Together with''': NRG2, NRG1, SIP1, SIP2<br>
| |
− | '''Phenotype(s)''': Loss of function (Null) [[Category:Phenotype:Loss of function (Null)]]
| |
− | | |
− | Deletion of SIP1 suppresses gal83's defect in invasive growth in response to glucose limitation. <ref name='S000072732'>Vyas VK, et al. (2003) Snf1 kinases with different beta-subunit isoforms play distinct roles in regulating haploid invasive growth. Mol Cell Biol 23(4):1341-8 {{SGDpaper|S000072732}} PMID 12556493</ref> <ref name = 'CAset6176-2003-03-27'>submitted by [http://db.yeastgenome.org/cgi-bin/colleague/colleagueSearch?id=6176 Valmik K. Vyas] on 2003-03-27</ref>
| |
− | | |
− | {{ShortCenteredHR}}
| |
− | | |
− | '''Strain Background''': Sigma1278b (Sigma2000 series)<br>
| |
− | '''Genotype''': MCY 4597<br>
| |
− | '''Together with''': NRG2, NRG1, SIP1, SIP2<br>
| |
− | '''Phenotype(s)''': Loss of function (Null) [[Category:Phenotype:Loss of function (Null)]]
| |
− | | |
− | sip2 gal83 double mutants are more defective for invasive growth in response to limiting glucose than gal83 single mutants. <ref name='S000072732'>Vyas VK, et al. (2003) Snf1 kinases with different beta-subunit isoforms play distinct roles in regulating haploid invasive growth. Mol Cell Biol 23(4):1341-8 {{SGDpaper|S000072732}} PMID 12556493</ref> <ref name = 'CAset6176-2003-03-27'>submitted by [http://db.yeastgenome.org/cgi-bin/colleague/colleagueSearch?id=6176 Valmik K. Vyas] on 2003-03-27</ref>
| |
− | | |
− | {{ShortCenteredHR}}
| |
− | | |
− | '''Strain Background''': Sigma1278b (Sigma 2000 series)<br>
| |
− | '''Genotype''': MCY4716<br>
| |
− | '''Together with''': NRG2, NRG1, SIP1, SIP2<br>
| |
− | '''Phenotype(s)''': Loss of function (Null) [[Category:Phenotype:Loss of function (Null)]]
| |
− | | |
− | Deletion of nrg1 partially suppresses gal83's defect in invasive growth, plastic adherance, and STA2-lacZ expression. <ref name='S000072732'>Vyas VK, et al. (2003) Snf1 kinases with different beta-subunit isoforms play distinct roles in regulating haploid invasive growth. Mol Cell Biol 23(4):1341-8 {{SGDpaper|S000072732}} PMID 12556493</ref> <ref name = 'CAset6176-2003-03-27'>submitted by [http://db.yeastgenome.org/cgi-bin/colleague/colleagueSearch?id=6176 Valmik K. Vyas] on 2003-03-27</ref>
| |
− | | |
− | {{ShortCenteredHR}}
| |
− | | |
− | '''Strain Background''': Sigma1278b (Sigma 2000 series)<br>
| |
− | '''Genotype''': MCY4708<br>
| |
− | '''Together with''': NRG2, NRG1, SIP1, SIP2<br>
| |
− | '''Phenotype(s)''': Loss of function (Null) [[Category:Phenotype:Loss of function (Null)]]
| |
− | | |
− | Deletion of nrg2 partially suppresses gal83's defect in invasive growth, plastic adherance, and STA2-lacZ expression. <ref name='S000072732'>Vyas VK, et al. (2003) Snf1 kinases with different beta-subunit isoforms play distinct roles in regulating haploid invasive growth. Mol Cell Biol 23(4):1341-8 {{SGDpaper|S000072732}} PMID 12556493</ref> <ref name = 'CAset6176-2003-03-27'>submitted by [http://db.yeastgenome.org/cgi-bin/colleague/colleagueSearch?id=6176 Valmik K. Vyas] on 2003-03-27</ref>
| |
− | | |
− | {{ShortCenteredHR}}
| |
− | | |
− | '''Strain Background''': Sigma1278b (Sigma2000 series)<br>
| |
− | '''Genotype''': MCY4600<br>
| |
− | '''Together with''': REG1, SIP1, SIP2, MUC1<br>
| |
− | '''Phenotype(s)''': Loss of function (Null) [[Category:Phenotype:Loss of function (Null)]]
| |
− | | |
− | Deletion of SIP1 in a gal83 sip2 double mutant causes an increase in invasion, suggesting Sip1 negatively regulates invasive growth. <ref name='S000072732'>Vyas VK, et al. (2003) Snf1 kinases with different beta-subunit isoforms play distinct roles in regulating haploid invasive growth. Mol Cell Biol 23(4):1341-8 {{SGDpaper|S000072732}} PMID 12556493</ref> <ref name = 'CAset6176-2003-03-27'>submitted by [http://db.yeastgenome.org/cgi-bin/colleague/colleagueSearch?id=6176 Valmik K. Vyas] on 2003-03-27</ref>
| |
− | | |
− | {{ShortCenteredHR}}
| |
− | | |
− | '''Strain Background''': Sigma1278b (Sigma2000 series)<br>
| |
− | '''Genotype''': MCY4571<br>
| |
− | '''Together with''': REG1, SIP1, SIP2, MUC1<br>
| |
− | '''Phenotype(s)''': Loss of function (Null) [[Category:Phenotype:Loss of function (Null)]]
| |
− | | |
− | Deletion of GAL83 suppresses reg1's constitutive adherance to plastic (adherance in high glucose (2%)). <ref name='S000072732'>Vyas VK, et al. (2003) Snf1 kinases with different beta-subunit isoforms play distinct roles in regulating haploid invasive growth. Mol Cell Biol 23(4):1341-8 {{SGDpaper|S000072732}} PMID 12556493</ref> <ref name = 'CAset6176-2003-03-27'>submitted by [http://db.yeastgenome.org/cgi-bin/colleague/colleagueSearch?id=6176 Valmik K. Vyas] on 2003-03-27</ref>
| |
− | | |
− | {{ShortCenteredHR}}
| |
− | | |
− | '''Strain Background''': Sigma1278b (Sigma 2000 series)<br>
| |
− | '''Genotype''': MCY4633<br>
| |
− | '''Together with''': REG1, SIP1, SIP2, MUC1<br>
| |
− | '''Phenotype(s)''': Loss of function (Null) [[Category:Phenotype:Loss of function (Null)]]
| |
− | | |
− | Deletion of both SIP2 and GAL83 in a reg1 mutant suppresses its constitutive filamentous phenotype. <ref name='S000072732'>Vyas VK, et al. (2003) Snf1 kinases with different beta-subunit isoforms play distinct roles in regulating haploid invasive growth. Mol Cell Biol 23(4):1341-8 {{SGDpaper|S000072732}} PMID 12556493</ref> <ref name = 'CAset6176-2003-03-27'>submitted by [http://db.yeastgenome.org/cgi-bin/colleague/colleagueSearch?id=6176 Valmik K. Vyas] on 2003-03-27</ref>
| |
− | | |
− | {{ShortCenteredHR}}
| |
− | | |
− | '''Strain Background''': Sigma1278b (Sigma2000 series)<br>
| |
− | '''Genotype''': MCY4600<br>
| |
− | '''Together with''': REG1, SIP1, SIP2, MUC1<br>
| |
− | '''Phenotype(s)''': Loss of function (Null) [[Category:Phenotype:Loss of function (Null)]]
| |
− | | |
− | Deletion of SIP1 in a gal83 sip2 double mutant causes an increase in invasion, suggesting Sip1 negatively regulates invasive growth. <ref name='S000072732'>Vyas VK, et al. (2003) Snf1 kinases with different beta-subunit isoforms play distinct roles in regulating haploid invasive growth. Mol Cell Biol 23(4):1341-8 {{SGDpaper|S000072732}} PMID 12556493</ref> <ref name = 'CAset6176-2003-03-27'>submitted by [http://db.yeastgenome.org/cgi-bin/colleague/colleagueSearch?id=6176 Valmik K. Vyas] on 2003-03-27</ref>
| |
− | | |
− | {{ShortCenteredHR}}
| |
− | | |
− | '''Strain Background''': Sigma1278b (Sigma2000 series)<br>
| |
− | '''Genotype''': MCY4571<br>
| |
− | '''Together with''': REG1, SIP1, SIP2, MUC1<br>
| |
− | '''Phenotype(s)''': Loss of function (Null) [[Category:Phenotype:Loss of function (Null)]]
| |
− | | |
− | Deletion of GAL83 suppresses reg1's constitutive adherance to plastic (adherance in high glucose (2%)). <ref name='S000072732'>Vyas VK, et al. (2003) Snf1 kinases with different beta-subunit isoforms play distinct roles in regulating haploid invasive growth. Mol Cell Biol 23(4):1341-8 {{SGDpaper|S000072732}} PMID 12556493</ref> <ref name = 'CAset6176-2003-03-27'>submitted by [http://db.yeastgenome.org/cgi-bin/colleague/colleagueSearch?id=6176 Valmik K. Vyas] on 2003-03-27</ref>
| |
− | | |
− | {{ShortCenteredHR}}
| |
− | | |
− | '''Strain Background''': Sigma1278b (Sigma 2000 series)<br>
| |
− | '''Genotype''': MCY4633<br>
| |
− | '''Together with''': REG1, SIP1, SIP2, MUC1<br>
| |
− | '''Phenotype(s)''': Loss of function (Null) [[Category:Phenotype:Loss of function (Null)]]
| |
− | | |
− | Deletion of both SIP2 and GAL83 in a reg1 mutant suppresses its constitutive filamentous phenotype. <ref name='S000072732'>Vyas VK, et al. (2003) Snf1 kinases with different beta-subunit isoforms play distinct roles in regulating haploid invasive growth. Mol Cell Biol 23(4):1341-8 {{SGDpaper|S000072732}} PMID 12556493</ref> <ref name = 'CAset6176-2003-03-27'>submitted by [http://db.yeastgenome.org/cgi-bin/colleague/colleagueSearch?id=6176 Valmik K. Vyas] on 2003-03-27</ref>
| |
− | | |
− | {{ShortCenteredHR}}
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− | | |
− | '''Strain Background''': Sigma1278b (Sigma 2000 series)<br>
| |
− | '''Genotype''': MCY4712<br>
| |
− | '''Together with''': NRG2, NRG1, SIP1, SIP2<br>
| |
− | '''Phenotype(s)''': Loss of function (Null) [[Category:Phenotype:Loss of function (Null)]]
| |
− | | |
− | Deletion of nrg1 and nrg2 suppresses gal83's defect in invasive growth, plastic adherance, and STA2-lacZ expression, better than deletion of either nrg alone. <ref name='S000072732'>Vyas VK, et al. (2003) Snf1 kinases with different beta-subunit isoforms play distinct roles in regulating haploid invasive growth. Mol Cell Biol 23(4):1341-8 {{SGDpaper|S000072732}} PMID 12556493</ref> <ref name = 'CAset6176-2003-03-27'>submitted by [http://db.yeastgenome.org/cgi-bin/colleague/colleagueSearch?id=6176 Valmik K. Vyas] on 2003-03-27</ref>
| |
− | | |
− | {{ShortCenteredHR}}
| |
− | | |
− | '''Strain Background''': Sigma1278b (Sigma 2000 series)<br>
| |
− | '''Genotype''': MCY4597<br>
| |
− | '''Together with''': NRG2, NRG1, SIP1, SIP2<br>
| |
− | '''Phenotype(s)''': Loss of function (Null) [[Category:Phenotype:Loss of function (Null)]]
| |
− | | |
− | sip2 gal83 double mutants are defective for filamentation in response to glucose depletion. <ref name='S000072732'>Vyas VK, et al. (2003) Snf1 kinases with different beta-subunit isoforms play distinct roles in regulating haploid invasive growth. Mol Cell Biol 23(4):1341-8 {{SGDpaper|S000072732}} PMID 12556493</ref> <ref name = 'CAset6176-2003-03-27'>submitted by [http://db.yeastgenome.org/cgi-bin/colleague/colleagueSearch?id=6176 Valmik K. Vyas] on 2003-03-27</ref>
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− | | |
− | {{ShortCenteredHR}}
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− | === Protein Details ===
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− | [[Category:Topic:Protein Details]]
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− | ==== Protein Function/Process ====
| |
− | [[Category:Topic:Protein Details:Protein Function/Process]]
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− | | |
− | One of three alternative beta subunits of the Snf1 kinase complex <ref name='S000059161'>Schmidt MC and McCartney RR (2000) beta-subunits of Snf1 kinase are required for kinase function and substrate definition. EMBO J 19(18):4936-43 {{SGDpaper|S000059161}} PMID 10990457</ref> <ref name = 'CAset5180-2003-02-17'>submitted by [http://db.yeastgenome.org/cgi-bin/colleague/colleagueSearch?id=5180 Martin C. Schmidt] on 2003-02-17</ref>
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− | | |
− | {{ShortCenteredHR}}
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| ==References== | | ==References== |
| <!-- REFERENCES ARE AUTOMATICALLY GENERATED. PLEASE DON'T EDIT THIS SECTION--> | | <!-- REFERENCES ARE AUTOMATICALLY GENERATED. PLEASE DON'T EDIT THIS SECTION--> |
| {{RefHelp}} | | {{RefHelp}} |
| + | </protect> |
Share your knowledge...Edit this entry!
<protect>
Systematic name |
YER027C
|
Gene name |
GAL83
|
Aliases |
SPM1
|
Feature type |
ORF, Verified
|
Coordinates
|
Chr V:210232..208979
|
Primary SGDID |
S000000829
|
Description of YER027C: One of three possible beta-subunits of the Snf1 kinase complex, allows nuclear localization of the Snf1 kinase complex in the presence of a nonfermentable carbon source; contains glycogen-binding domain[1][2]
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Alleles, Strains, and Phenotypes
Complete Deletion
Allele: gal83delta
Strain Background: Sigma1278b (Sigma2000 series)
Genotype: MCY4567
Together with: NRG2, NRG1, SIP1, SIP2
Phenotype(s): Loss of function (Null)
gal83 mutants are defective in adherance to plastic in response to limiting glucose. [3] [4]
Allele: gal83delta
Strain Background: Sigma1278b
Genotype: MCY4567
Together with: NRG2, NRG1, SIP1, SIP2
Phenotype(s): Loss of function (Null)
gal83 mutants are defective in invasive growth in response to limiting glucose. [3] [4]
Allele: gal83delta
Strain Background: Sigma1278b (Sigma2000 series)
Genotype: MCY4567
Together with: NRG2, NRG1, SIP1, SIP2
Phenotype(s): Loss of function (Null)
gal83 mutants are defective in adherance to plastic in response to limiting glucose. [3] [4]
Allele: gal83 delta
Strain Background: Sigma1278b (Sigma2000 series)
Genotype: MCY 4567
Together with: NRG2, NRG1, SIP1, SIP2
Phenotype(s): Loss of function (Null)
gal83 mutants exhibit wild-type filamentation in response to glucose depletion. [3] [4]
Allele: gal83delta
Strain Background: Sigma1278b
Genotype: MCY4567
Together with: NRG2, NRG1, SIP1, SIP2
Phenotype(s): Loss of function (Null)
gal83 mutants are defective in invasive growth in response to limiting glucose. [3] [4]
Allele: gal83 delta
Strain Background: Sigma1278b (Sigma2000 series)
Genotype: MCY 4567
Together with: NRG2, NRG1, SIP1, SIP2
Phenotype(s): Loss of function (Null)
gal83 mutants exhibit wild-type filamentation in response to glucose depletion. [3] [4]
Multiple Knockout Strains
Strain Background: Sigma1278b (Sigma 2000 series)
Genotype: MCY4708
Together with: NRG2, NRG1, SIP1, SIP2
Phenotype(s): Loss of function (Null)
Deletion of nrg2 partially suppresses gal83's defect in invasive growth, plastic adherance, and STA2-lacZ expression. [3] [4]
Strain Background: Sigma1278b (Sigma 2000 series)
Genotype: MCY4716
Together with: NRG2, NRG1, SIP1, SIP2
Phenotype(s): Loss of function (Null)
Deletion of nrg1 partially suppresses gal83's defect in invasive growth, plastic adherance, and STA2-lacZ expression. [3] [4]
Strain Background: Sigma1278b (Sigma2000 series)
Genotype: MCY 4597
Together with: NRG2, NRG1, SIP1, SIP2
Phenotype(s): Loss of function (Null)
sip2 gal83 double mutants are more defective for invasive growth in response to limiting glucose than gal83 single mutants. [3] [4]
Strain Background: Sigma1278b (Sigma2000 series)
Genotype: MCY4581
Together with: NRG2, NRG1, SIP1, SIP2
Phenotype(s): Loss of function (Null)
Deletion of SIP1 suppresses gal83's defect in invasive growth in response to glucose limitation. [3] [4]
Strain Background: Sigma1278b (Sigma 2000 series)
Genotype: MCY4712
Together with: NRG2, NRG1, SIP1, SIP2
Phenotype(s): Loss of function (Null)
Deletion of nrg1 and nrg2 suppresses gal83's defect in invasive growth, plastic adherance, and STA2-lacZ expression, better than deletion of either nrg alone. [3] [4]
Strain Background: Sigma1278b (Sigma 2000 series)
Genotype: MCY4597
Together with: NRG2, NRG1, SIP1, SIP2
Phenotype(s): Loss of function (Null)
sip2 gal83 double mutants are defective for filamentation in response to glucose depletion. [3] [4]
Strain Background: Sigma1278b (Sigma2000 series)
Genotype: MCY4581
Together with: NRG2, NRG1, SIP1, SIP2
Phenotype(s): Loss of function (Null)
Deletion of SIP1 suppresses gal83's defect in invasive growth in response to glucose limitation. [3] [4]
Strain Background: Sigma1278b (Sigma2000 series)
Genotype: MCY 4597
Together with: NRG2, NRG1, SIP1, SIP2
Phenotype(s): Loss of function (Null)
sip2 gal83 double mutants are more defective for invasive growth in response to limiting glucose than gal83 single mutants. [3] [4]
Strain Background: Sigma1278b (Sigma 2000 series)
Genotype: MCY4716
Together with: NRG2, NRG1, SIP1, SIP2
Phenotype(s): Loss of function (Null)
Deletion of nrg1 partially suppresses gal83's defect in invasive growth, plastic adherance, and STA2-lacZ expression. [3] [4]
Strain Background: Sigma1278b (Sigma 2000 series)
Genotype: MCY4708
Together with: NRG2, NRG1, SIP1, SIP2
Phenotype(s): Loss of function (Null)
Deletion of nrg2 partially suppresses gal83's defect in invasive growth, plastic adherance, and STA2-lacZ expression. [3] [4]
Strain Background: Sigma1278b (Sigma2000 series)
Genotype: MCY4600
Together with: REG1, SIP1, SIP2, MUC1
Phenotype(s): Loss of function (Null)
Deletion of SIP1 in a gal83 sip2 double mutant causes an increase in invasion, suggesting Sip1 negatively regulates invasive growth. [3] [4]
Strain Background: Sigma1278b (Sigma2000 series)
Genotype: MCY4571
Together with: REG1, SIP1, SIP2, MUC1
Phenotype(s): Loss of function (Null)
Deletion of GAL83 suppresses reg1's constitutive adherance to plastic (adherance in high glucose (2%)). [3] [4]
Strain Background: Sigma1278b (Sigma 2000 series)
Genotype: MCY4633
Together with: REG1, SIP1, SIP2, MUC1
Phenotype(s): Loss of function (Null)
Deletion of both SIP2 and GAL83 in a reg1 mutant suppresses its constitutive filamentous phenotype. [3] [4]
Strain Background: Sigma1278b (Sigma2000 series)
Genotype: MCY4600
Together with: REG1, SIP1, SIP2, MUC1
Phenotype(s): Loss of function (Null)
Deletion of SIP1 in a gal83 sip2 double mutant causes an increase in invasion, suggesting Sip1 negatively regulates invasive growth. [3] [4]
Strain Background: Sigma1278b (Sigma2000 series)
Genotype: MCY4571
Together with: REG1, SIP1, SIP2, MUC1
Phenotype(s): Loss of function (Null)
Deletion of GAL83 suppresses reg1's constitutive adherance to plastic (adherance in high glucose (2%)). [3] [4]
Strain Background: Sigma1278b (Sigma 2000 series)
Genotype: MCY4633
Together with: REG1, SIP1, SIP2, MUC1
Phenotype(s): Loss of function (Null)
Deletion of both SIP2 and GAL83 in a reg1 mutant suppresses its constitutive filamentous phenotype. [3] [4]
Strain Background: Sigma1278b (Sigma 2000 series)
Genotype: MCY4712
Together with: NRG2, NRG1, SIP1, SIP2
Phenotype(s): Loss of function (Null)
Deletion of nrg1 and nrg2 suppresses gal83's defect in invasive growth, plastic adherance, and STA2-lacZ expression, better than deletion of either nrg alone. [3] [4]
Strain Background: Sigma1278b (Sigma 2000 series)
Genotype: MCY4597
Together with: NRG2, NRG1, SIP1, SIP2
Phenotype(s): Loss of function (Null)
sip2 gal83 double mutants are defective for filamentation in response to glucose depletion. [3] [4]
Protein Details
Protein Function/Process
One of three alternative beta subunits of the Snf1 kinase complex [5] [6]
<protect>
References
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</protect>