YER027C

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Systematic name YER027C
Gene name GAL83
Aliases SPM1
Feature type ORF, Verified
Coordinates Chr V:210232..208979
Primary SGDID S000000829


Description of YER027C: One of three possible beta-subunits of the Snf1 kinase complex, allows nuclear localization of the Snf1 kinase complex in the presence of a nonfermentable carbon source; contains glycogen-binding domain[1][2]




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Alleles, Strains, and Phenotypes

Complete Deletion

Allele: gal83delta

Strain Background: Sigma1278b (Sigma2000 series)
Genotype: MCY4567
Together with: NRG2, NRG1, SIP1, SIP2
Phenotype(s): Loss of function (Null)

gal83 mutants are defective in adherance to plastic in response to limiting glucose. [3] [4]


Allele: gal83delta

Strain Background: Sigma1278b
Genotype: MCY4567
Together with: NRG2, NRG1, SIP1, SIP2
Phenotype(s): Loss of function (Null)

gal83 mutants are defective in invasive growth in response to limiting glucose. [3] [4]


Allele: gal83delta

Strain Background: Sigma1278b (Sigma2000 series)
Genotype: MCY4567
Together with: NRG2, NRG1, SIP1, SIP2
Phenotype(s): Loss of function (Null)

gal83 mutants are defective in adherance to plastic in response to limiting glucose. [3] [4]


Allele: gal83 delta

Strain Background: Sigma1278b (Sigma2000 series)
Genotype: MCY 4567
Together with: NRG2, NRG1, SIP1, SIP2
Phenotype(s): Loss of function (Null)

gal83 mutants exhibit wild-type filamentation in response to glucose depletion. [3] [4]


Allele: gal83delta

Strain Background: Sigma1278b
Genotype: MCY4567
Together with: NRG2, NRG1, SIP1, SIP2
Phenotype(s): Loss of function (Null)

gal83 mutants are defective in invasive growth in response to limiting glucose. [3] [4]


Allele: gal83 delta

Strain Background: Sigma1278b (Sigma2000 series)
Genotype: MCY 4567
Together with: NRG2, NRG1, SIP1, SIP2
Phenotype(s): Loss of function (Null)

gal83 mutants exhibit wild-type filamentation in response to glucose depletion. [3] [4]


Multiple Knockout Strains

Strain Background: Sigma1278b (Sigma 2000 series)
Genotype: MCY4708
Together with: NRG2, NRG1, SIP1, SIP2
Phenotype(s): Loss of function (Null)

Deletion of nrg2 partially suppresses gal83's defect in invasive growth, plastic adherance, and STA2-lacZ expression. [3] [4]


Strain Background: Sigma1278b (Sigma 2000 series)
Genotype: MCY4716
Together with: NRG2, NRG1, SIP1, SIP2
Phenotype(s): Loss of function (Null)

Deletion of nrg1 partially suppresses gal83's defect in invasive growth, plastic adherance, and STA2-lacZ expression. [3] [4]


Strain Background: Sigma1278b (Sigma2000 series)
Genotype: MCY 4597
Together with: NRG2, NRG1, SIP1, SIP2
Phenotype(s): Loss of function (Null)

sip2 gal83 double mutants are more defective for invasive growth in response to limiting glucose than gal83 single mutants. [3] [4]


Strain Background: Sigma1278b (Sigma2000 series)
Genotype: MCY4581
Together with: NRG2, NRG1, SIP1, SIP2
Phenotype(s): Loss of function (Null)

Deletion of SIP1 suppresses gal83's defect in invasive growth in response to glucose limitation. [3] [4]


Strain Background: Sigma1278b (Sigma 2000 series)
Genotype: MCY4712
Together with: NRG2, NRG1, SIP1, SIP2
Phenotype(s): Loss of function (Null)

Deletion of nrg1 and nrg2 suppresses gal83's defect in invasive growth, plastic adherance, and STA2-lacZ expression, better than deletion of either nrg alone. [3] [4]


Strain Background: Sigma1278b (Sigma 2000 series)
Genotype: MCY4597
Together with: NRG2, NRG1, SIP1, SIP2
Phenotype(s): Loss of function (Null)

sip2 gal83 double mutants are defective for filamentation in response to glucose depletion. [3] [4]


Strain Background: Sigma1278b (Sigma2000 series)
Genotype: MCY4581
Together with: NRG2, NRG1, SIP1, SIP2
Phenotype(s): Loss of function (Null)

Deletion of SIP1 suppresses gal83's defect in invasive growth in response to glucose limitation. [3] [4]


Strain Background: Sigma1278b (Sigma2000 series)
Genotype: MCY 4597
Together with: NRG2, NRG1, SIP1, SIP2
Phenotype(s): Loss of function (Null)

sip2 gal83 double mutants are more defective for invasive growth in response to limiting glucose than gal83 single mutants. [3] [4]


Strain Background: Sigma1278b (Sigma 2000 series)
Genotype: MCY4716
Together with: NRG2, NRG1, SIP1, SIP2
Phenotype(s): Loss of function (Null)

Deletion of nrg1 partially suppresses gal83's defect in invasive growth, plastic adherance, and STA2-lacZ expression. [3] [4]


Strain Background: Sigma1278b (Sigma 2000 series)
Genotype: MCY4708
Together with: NRG2, NRG1, SIP1, SIP2
Phenotype(s): Loss of function (Null)

Deletion of nrg2 partially suppresses gal83's defect in invasive growth, plastic adherance, and STA2-lacZ expression. [3] [4]


Strain Background: Sigma1278b (Sigma2000 series)
Genotype: MCY4600
Together with: REG1, SIP1, SIP2, MUC1
Phenotype(s): Loss of function (Null)

Deletion of SIP1 in a gal83 sip2 double mutant causes an increase in invasion, suggesting Sip1 negatively regulates invasive growth. [3] [4]


Strain Background: Sigma1278b (Sigma2000 series)
Genotype: MCY4571
Together with: REG1, SIP1, SIP2, MUC1
Phenotype(s): Loss of function (Null)

Deletion of GAL83 suppresses reg1's constitutive adherance to plastic (adherance in high glucose (2%)). [3] [4]


Strain Background: Sigma1278b (Sigma 2000 series)
Genotype: MCY4633
Together with: REG1, SIP1, SIP2, MUC1
Phenotype(s): Loss of function (Null)

Deletion of both SIP2 and GAL83 in a reg1 mutant suppresses its constitutive filamentous phenotype. [3] [4]


Strain Background: Sigma1278b (Sigma2000 series)
Genotype: MCY4600
Together with: REG1, SIP1, SIP2, MUC1
Phenotype(s): Loss of function (Null)

Deletion of SIP1 in a gal83 sip2 double mutant causes an increase in invasion, suggesting Sip1 negatively regulates invasive growth. [3] [4]


Strain Background: Sigma1278b (Sigma2000 series)
Genotype: MCY4571
Together with: REG1, SIP1, SIP2, MUC1
Phenotype(s): Loss of function (Null)

Deletion of GAL83 suppresses reg1's constitutive adherance to plastic (adherance in high glucose (2%)). [3] [4]


Strain Background: Sigma1278b (Sigma 2000 series)
Genotype: MCY4633
Together with: REG1, SIP1, SIP2, MUC1
Phenotype(s): Loss of function (Null)

Deletion of both SIP2 and GAL83 in a reg1 mutant suppresses its constitutive filamentous phenotype. [3] [4]


Strain Background: Sigma1278b (Sigma 2000 series)
Genotype: MCY4712
Together with: NRG2, NRG1, SIP1, SIP2
Phenotype(s): Loss of function (Null)

Deletion of nrg1 and nrg2 suppresses gal83's defect in invasive growth, plastic adherance, and STA2-lacZ expression, better than deletion of either nrg alone. [3] [4]


Strain Background: Sigma1278b (Sigma 2000 series)
Genotype: MCY4597
Together with: NRG2, NRG1, SIP1, SIP2
Phenotype(s): Loss of function (Null)

sip2 gal83 double mutants are defective for filamentation in response to glucose depletion. [3] [4]


Protein Details

Protein Function/Process

One of three alternative beta subunits of the Snf1 kinase complex [5] [6]





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References

See Help:References on how to add references

  1. Schuller HJ (2003) Transcriptional control of nonfermentative metabolism in the yeast Saccharomyces cerevisiae. Curr Genet 43(3):139-60 SGD PMID 12715202
  2. Wiatrowski HA, et al. (2004) Mutations in the gal83 glycogen-binding domain activate the snf1/gal83 kinase pathway by a glycogen-independent mechanism. Mol Cell Biol 24(1):352-61 SGD PMID 14673168
  3. 3.00 3.01 3.02 3.03 3.04 3.05 3.06 3.07 3.08 3.09 3.10 3.11 3.12 3.13 3.14 3.15 3.16 3.17 3.18 3.19 3.20 3.21 3.22 3.23 Vyas VK, et al. (2003) Snf1 kinases with different beta-subunit isoforms play distinct roles in regulating haploid invasive growth. Mol Cell Biol 23(4):1341-8 SGD PMID 12556493
  4. 4.00 4.01 4.02 4.03 4.04 4.05 4.06 4.07 4.08 4.09 4.10 4.11 4.12 4.13 4.14 4.15 4.16 4.17 4.18 4.19 4.20 4.21 4.22 4.23 submitted by Valmik K. Vyas on 2003-03-27
  5. Schmidt MC and McCartney RR (2000) beta-subunits of Snf1 kinase are required for kinase function and substrate definition. EMBO J 19(18):4936-43 SGD PMID 10990457
  6. submitted by Martin C. Schmidt on 2003-02-17

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