Difference between revisions of "YBR066C"

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|valign="top" nowrap bgcolor="{{SGDblue}}"| '''Systematic name''' || [http://db.yeastgenome.org/cgi-bin/locus.pl?locus=YBR066C YBR066C]  
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|valign="top" nowrap bgcolor="{{SGDblue}}"| '''Systematic name''' || [http://www.yeastgenome.org/cgi-bin/locus.pl?dbid=S000000270 YBR066C]  
 
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|valign="top" nowrap bgcolor="{{SGDblue}}"| '''Gene name'''        ||''NRG2 ''
 
|valign="top" nowrap bgcolor="{{SGDblue}}"| '''Gene name'''        ||''NRG2 ''
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|valign="top" nowrap bgcolor="{{SGDblue}}"| '''Coordinates'''
 
|valign="top" nowrap bgcolor="{{SGDblue}}"| '''Coordinates'''
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|nowrap| Chr II:370697..370035
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|nowrap| Chr II:370699..370037
 
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|valign="top" nowrap bgcolor="{{SGDblue}}"| '''Primary SGDID'''          || S000000270
 
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'''Description of {{PAGENAME}}:''' Transcriptional repressor that mediates glucose repression and negatively regulates filamentous growth; has similarity to Nrg1p<ref name='S000070028'>Kuchin S, et al. (2002) Snf1 protein kinase and the repressors Nrg1 and Nrg2 regulate FLO11, haploid invasive growth, and diploid pseudohyphal differentiation. Mol Cell Biol 22(12):3994-4000 {{SGDpaper|S000070028}} PMID 12024013</ref><ref name='S000060795'>Vyas VK, et al. (2001) Interaction of the repressors Nrg1 and Nrg2 with the Snf1 protein kinase in Saccharomyces cerevisiae. Genetics 158(2):563-72
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'''Description of YBR066C:''' Transcriptional repressor that mediates glucose repression and negatively regulates filamentous growth; has similarity to Nrg1p<ref name='S000070028'>Kuchin S, et al. (2002) Snf1 protein kinase and the repressors Nrg1 and Nrg2 regulate FLO11, haploid invasive growth, and diploid pseudohyphal differentiation. Mol Cell Biol 22(12):3994-4000 {{SGDpaper|S000070028}} PMID 12024013</ref><ref name='S000060795'>Vyas VK, et al. (2001) Interaction of the repressors Nrg1 and Nrg2 with the Snf1 protein kinase in Saccharomyces cerevisiae. Genetics 158(2):563-72
 
  {{SGDpaper|S000060795}} PMID 11404322</ref>
 
  {{SGDpaper|S000060795}} PMID 11404322</ref>
 
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__TOC__
 
__TOC__
 
==Community Commentary==
 
==Community Commentary==
 
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=== Alleles, Strains, and Phenotypes ===
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[[Category:Topic:Alleles, Strains, and Phenotypes]]
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==== Multiple Knockout Strains ====
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[[Category:Topic:Alleles, Strains, and Phenotypes:Multiple Knockout Strains]]
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'''Strain Background''': Sigma1278b (Sigma 2000 series)<br>
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'''Genotype''': MCY4708<br>
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'''Together with''': NRG1, SIP1, GAL83, SIP2<br>
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'''Phenotype(s)''': Loss of function (Null) [[Category:Phenotype:Loss of function (Null)]]
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Deletion of nrg2 partially suppresses gal83's defect in invasive growth, plastic adherance, and STA2-lacZ expression. <ref name='S000072732'>Vyas VK, et al. (2003) Snf1 kinases with different beta-subunit isoforms play distinct roles in regulating haploid invasive growth. Mol Cell Biol 23(4):1341-8 {{SGDpaper|S000072732}} PMID 12556493</ref> <ref name = 'CAset6176-2003-03-27'>submitted by [http://db.yeastgenome.org/cgi-bin/colleague/colleagueSearch?id=6176 Valmik K. Vyas] on 2003-03-27</ref>
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{{ShortCenteredHR}}
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'''Strain Background''': Sigma1278b (Sigma 2000 series)<br>
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'''Genotype''': MCY4712<br>
 +
'''Together with''': NRG1, SIP1, GAL83, SIP2<br>
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'''Phenotype(s)''': Loss of function (Null) [[Category:Phenotype:Loss of function (Null)]]
 +
 +
Deletion of nrg1 and nrg2 suppresses gal83's defect in invasive growth, plastic adherance, and STA2-lacZ expression, better than deletion of either nrg alone. <ref name='S000072732'>Vyas VK, et al. (2003) Snf1 kinases with different beta-subunit isoforms play distinct roles in regulating haploid invasive growth. Mol Cell Biol 23(4):1341-8 {{SGDpaper|S000072732}} PMID 12556493</ref> <ref name = 'CAset6176-2003-03-27'>submitted by [http://db.yeastgenome.org/cgi-bin/colleague/colleagueSearch?id=6176 Valmik K. Vyas] on 2003-03-27</ref>
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{{ShortCenteredHR}}
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'''Strain Background''': Sigma1278b (Sigma 2000 series)<br>
 +
'''Genotype''': MCY4708<br>
 +
'''Together with''': NRG1, SIP1, GAL83, SIP2<br>
 +
'''Phenotype(s)''': Loss of function (Null) [[Category:Phenotype:Loss of function (Null)]]
 +
 +
Deletion of nrg2 partially suppresses gal83's defect in invasive growth, plastic adherance, and STA2-lacZ expression. <ref name='S000072732'>Vyas VK, et al. (2003) Snf1 kinases with different beta-subunit isoforms play distinct roles in regulating haploid invasive growth. Mol Cell Biol 23(4):1341-8 {{SGDpaper|S000072732}} PMID 12556493</ref> <ref name = 'CAset6176-2003-03-27'>submitted by [http://db.yeastgenome.org/cgi-bin/colleague/colleagueSearch?id=6176 Valmik K. Vyas] on 2003-03-27</ref>
 +
 +
{{ShortCenteredHR}}
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 +
'''Strain Background''': Sigma1278b (Sigma 2000 series)<br>
 +
'''Genotype''': MCY4712<br>
 +
'''Together with''': NRG1, SIP1, GAL83, SIP2<br>
 +
'''Phenotype(s)''': Loss of function (Null) [[Category:Phenotype:Loss of function (Null)]]
 +
 +
Deletion of nrg1 and nrg2 suppresses gal83's defect in invasive growth, plastic adherance, and STA2-lacZ expression, better than deletion of either nrg alone. <ref name='S000072732'>Vyas VK, et al. (2003) Snf1 kinases with different beta-subunit isoforms play distinct roles in regulating haploid invasive growth. Mol Cell Biol 23(4):1341-8 {{SGDpaper|S000072732}} PMID 12556493</ref> <ref name = 'CAset6176-2003-03-27'>submitted by [http://db.yeastgenome.org/cgi-bin/colleague/colleagueSearch?id=6176 Valmik K. Vyas] on 2003-03-27</ref>
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{{ShortCenteredHR}}
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==== Overexpression ====
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[[Category:Topic:Alleles, Strains, and Phenotypes:Overexpression]]
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'''Phenotype(s)''': Dominant [[Category:Phenotype:Dominant]], Gain of function [[Category:Phenotype:Gain of function]]
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Overexpression of VP16-Nrg2 results in partial derepression of SUC2 in glucose grown cells. <ref name='S000060795'>Vyas VK, et al. (2001) Interaction of the repressors Nrg1 and Nrg2 with the Snf1 protein kinase in Saccharomyces cerevisiae. Genetics 158(2):563-72 {{SGDpaper|S000060795}} PMID 11404322</ref> <ref name = 'CAset6176-2003-02-04'>submitted by [http://db.yeastgenome.org/cgi-bin/colleague/colleagueSearch?id=6176 Valmik K. Vyas] on 2003-02-04</ref>
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{{ShortCenteredHR}}
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=== Interactions ===
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[[Category:Topic:Interactions]]
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==== Genetic ====
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[[Category:Topic:Interactions:Genetic]]
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YBR066C is a suppressor of  Snf1 [[Category:Genetic Interaction with Snf1]]
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'''Strain Background''': Sigma 1278b<br>
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'''Mutation type(s)''': complete deletion, complete deletion
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Deletion of Nrg2 partially suppresses Snf1's defect in invasive growth, biofilm formation, FLO11 expression, and STA2 expression. <ref name='S000070028'>Kuchin S, et al. (2002) Snf1 protein kinase and the repressors Nrg1 and Nrg2 regulate FLO11, haploid invasive growth, and diploid pseudohyphal differentiation. Mol Cell Biol 22(12):3994-4000 {{SGDpaper|S000070028}} PMID 12024013</ref> <ref name = 'CAset6176-2003-02-04'>submitted by [http://db.yeastgenome.org/cgi-bin/colleague/colleagueSearch?id=6176 Valmik K. Vyas] on 2003-02-04</ref>
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{{ShortCenteredHR}}
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==== Physical ====
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[[Category:Topic:Interactions:Physical]]
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Physical interaction with Snf1 [[Category:Physical Interactions with Snf1]]<br>
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LexA-Snf1 coimmunoprecipitates HA3-Nrg2 <ref name='S000060795'>Vyas VK, et al. (2001) Interaction of the repressors Nrg1 and Nrg2 with the Snf1 protein kinase in Saccharomyces cerevisiae. Genetics 158(2):563-72 {{SGDpaper|S000060795}} PMID 11404322</ref> <ref name = 'CAset6176-2003-02-04'>submitted by [http://db.yeastgenome.org/cgi-bin/colleague/colleagueSearch?id=6176 Valmik K. Vyas] on 2003-02-04</ref>
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{{ShortCenteredHR}}
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Physical interaction with Snf1 [[Category:Physical Interactions with Snf1]]<br>
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Snf1 coimmunoprecipitates with HA3-Nrg2 <ref name='S000060795'>Vyas VK, et al. (2001) Interaction of the repressors Nrg1 and Nrg2 with the Snf1 protein kinase in Saccharomyces cerevisiae. Genetics 158(2):563-72 {{SGDpaper|S000060795}} PMID 11404322</ref> <ref name = 'CAset6176-2003-02-04'>submitted by [http://db.yeastgenome.org/cgi-bin/colleague/colleagueSearch?id=6176 Valmik K. Vyas] on 2003-02-04</ref>
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{{ShortCenteredHR}}
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==== Two Hybrid ====
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[[Category:Topic:Interactions:Two Hybrid]]
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Two Hybrid interaction with Snf1 [[Category:Two Hybrid Interactions with Snf1]]<br>
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Snf1 kinase domain interacts with Nrg2 <ref name='S000060795'>Vyas VK, et al. (2001) Interaction of the repressors Nrg1 and Nrg2 with the Snf1 protein kinase in Saccharomyces cerevisiae. Genetics 158(2):563-72 {{SGDpaper|S000060795}} PMID 11404322</ref> <ref name = 'CAset6176-2003-02-04'>submitted by [http://db.yeastgenome.org/cgi-bin/colleague/colleagueSearch?id=6176 Valmik K. Vyas] on 2003-02-04</ref>
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{{ShortCenteredHR}}
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=== Protein Details ===
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[[Category:Topic:Protein Details]]
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==== Protein Function/Process ====
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[[Category:Topic:Protein Details:Protein Function/Process]]
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LexA-Nrg2 represses transcription from a heterologous promoter containing LexA binding sites. <ref name='S000060795'>Vyas VK, et al. (2001) Interaction of the repressors Nrg1 and Nrg2 with the Snf1 protein kinase in Saccharomyces cerevisiae. Genetics 158(2):563-72 {{SGDpaper|S000060795}} PMID 11404322</ref> <ref name = 'CAset6176-2003-02-04'>submitted by [http://db.yeastgenome.org/cgi-bin/colleague/colleagueSearch?id=6176 Valmik K. Vyas] on 2003-02-04</ref>
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{{ShortCenteredHR}}
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Specifically higher expression in carbon limited chemostat cultures versus carbon excess.
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<ref>Boer VM, et al. (2003) The genome-wide transcriptional responses of Saccharomyces cerevisiae grown on glucose in aerobic chemostat cultures limited for carbon, nitrogen, phosphorus, or sulfur.
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J Biol Chem 278(5):3265-74</ref>
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==References==
 
==References==
 
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Latest revision as of 07:45, 23 January 2012

Share your knowledge...Edit this entry! <protect>

Systematic name YBR066C
Gene name NRG2
Aliases
Feature type ORF, Verified
Coordinates Chr II:370699..370037
Primary SGDID S000000270


Description of YBR066C: Transcriptional repressor that mediates glucose repression and negatively regulates filamentous growth; has similarity to Nrg1p[1][2]




</protect>

Community Commentary

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Alleles, Strains, and Phenotypes

Multiple Knockout Strains

Strain Background: Sigma1278b (Sigma 2000 series)
Genotype: MCY4708
Together with: NRG1, SIP1, GAL83, SIP2
Phenotype(s): Loss of function (Null)

Deletion of nrg2 partially suppresses gal83's defect in invasive growth, plastic adherance, and STA2-lacZ expression. [3] [4]

Strain Background: Sigma1278b (Sigma 2000 series)
Genotype: MCY4712
Together with: NRG1, SIP1, GAL83, SIP2
Phenotype(s): Loss of function (Null)

Deletion of nrg1 and nrg2 suppresses gal83's defect in invasive growth, plastic adherance, and STA2-lacZ expression, better than deletion of either nrg alone. [3] [4]

Strain Background: Sigma1278b (Sigma 2000 series)
Genotype: MCY4708
Together with: NRG1, SIP1, GAL83, SIP2
Phenotype(s): Loss of function (Null)

Deletion of nrg2 partially suppresses gal83's defect in invasive growth, plastic adherance, and STA2-lacZ expression. [3] [4]

Strain Background: Sigma1278b (Sigma 2000 series)
Genotype: MCY4712
Together with: NRG1, SIP1, GAL83, SIP2
Phenotype(s): Loss of function (Null)

Deletion of nrg1 and nrg2 suppresses gal83's defect in invasive growth, plastic adherance, and STA2-lacZ expression, better than deletion of either nrg alone. [3] [4]

Overexpression

Phenotype(s): Dominant, Gain of function

Overexpression of VP16-Nrg2 results in partial derepression of SUC2 in glucose grown cells. [2] [5]

Interactions

Genetic

YBR066C is a suppressor of Snf1


Strain Background: Sigma 1278b
Mutation type(s): complete deletion, complete deletion

Deletion of Nrg2 partially suppresses Snf1's defect in invasive growth, biofilm formation, FLO11 expression, and STA2 expression. [1] [5]

Physical

Physical interaction with Snf1
LexA-Snf1 coimmunoprecipitates HA3-Nrg2 [2] [5]

Physical interaction with Snf1
Snf1 coimmunoprecipitates with HA3-Nrg2 [2] [5]

Two Hybrid

Two Hybrid interaction with Snf1
Snf1 kinase domain interacts with Nrg2 [2] [5]

Protein Details

Protein Function/Process

LexA-Nrg2 represses transcription from a heterologous promoter containing LexA binding sites. [2] [5]




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References

See Help:References on how to add references

  1. ↑ 1.0 1.1 Kuchin S, et al. (2002) Snf1 protein kinase and the repressors Nrg1 and Nrg2 regulate FLO11, haploid invasive growth, and diploid pseudohyphal differentiation. Mol Cell Biol 22(12):3994-4000 SGD PMID 12024013
  2. ↑ 2.0 2.1 2.2 2.3 2.4 2.5 Vyas VK, et al. (2001) Interaction of the repressors Nrg1 and Nrg2 with the Snf1 protein kinase in Saccharomyces cerevisiae. Genetics 158(2):563-72 SGD PMID 11404322 Cite error: Invalid <ref> tag; name "S000060795" defined multiple times with different content Cite error: Invalid <ref> tag; name "S000060795" defined multiple times with different content Cite error: Invalid <ref> tag; name "S000060795" defined multiple times with different content Cite error: Invalid <ref> tag; name "S000060795" defined multiple times with different content Cite error: Invalid <ref> tag; name "S000060795" defined multiple times with different content
  3. ↑ 3.0 3.1 3.2 3.3 Vyas VK, et al. (2003) Snf1 kinases with different beta-subunit isoforms play distinct roles in regulating haploid invasive growth. Mol Cell Biol 23(4):1341-8 SGD PMID 12556493
  4. ↑ 4.0 4.1 4.2 4.3 submitted by Valmik K. Vyas on 2003-03-27
  5. ↑ 5.0 5.1 5.2 5.3 5.4 5.5 submitted by Valmik K. Vyas on 2003-02-04

See Help:Categories on how to add the wiki page for this gene to a Category </protect>

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