YBR066C

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Systematic name YBR066C
Gene name NRG2
Aliases
Feature type ORF, Verified
Coordinates Chr II:370699..370037
Primary SGDID S000000270


Description of YBR066C: Transcriptional repressor that mediates glucose repression and negatively regulates filamentous growth; has similarity to Nrg1p[1][2]




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Alleles, Strains, and Phenotypes

Multiple Knockout Strains

Strain Background: Sigma1278b (Sigma 2000 series)
Genotype: MCY4708
Together with: NRG1, SIP1, GAL83, SIP2
Phenotype(s): Loss of function (Null)

Deletion of nrg2 partially suppresses gal83's defect in invasive growth, plastic adherance, and STA2-lacZ expression. [3] [4]


Strain Background: Sigma1278b (Sigma 2000 series)
Genotype: MCY4712
Together with: NRG1, SIP1, GAL83, SIP2
Phenotype(s): Loss of function (Null)

Deletion of nrg1 and nrg2 suppresses gal83's defect in invasive growth, plastic adherance, and STA2-lacZ expression, better than deletion of either nrg alone. [3] [4]


Strain Background: Sigma1278b (Sigma 2000 series)
Genotype: MCY4708
Together with: NRG1, SIP1, GAL83, SIP2
Phenotype(s): Loss of function (Null)

Deletion of nrg2 partially suppresses gal83's defect in invasive growth, plastic adherance, and STA2-lacZ expression. [3] [4]


Strain Background: Sigma1278b (Sigma 2000 series)
Genotype: MCY4712
Together with: NRG1, SIP1, GAL83, SIP2
Phenotype(s): Loss of function (Null)

Deletion of nrg1 and nrg2 suppresses gal83's defect in invasive growth, plastic adherance, and STA2-lacZ expression, better than deletion of either nrg alone. [3] [4]


Overexpression

Phenotype(s): Dominant, Gain of function

Overexpression of VP16-Nrg2 results in partial derepression of SUC2 in glucose grown cells. [2] [5]


Interactions

Genetic

YBR066C is a suppressor of Snf1


Strain Background: Sigma 1278b
Mutation type(s): complete deletion, complete deletion

Deletion of Nrg2 partially suppresses Snf1's defect in invasive growth, biofilm formation, FLO11 expression, and STA2 expression. [1] [5]


Physical

Physical interaction with Snf1
LexA-Snf1 coimmunoprecipitates HA3-Nrg2 [2] [5]


Physical interaction with Snf1
Snf1 coimmunoprecipitates with HA3-Nrg2 [2] [5]


Two Hybrid

Two Hybrid interaction with Snf1
Snf1 kinase domain interacts with Nrg2 [2] [5]


Protein Details

Protein Function/Process

LexA-Nrg2 represses transcription from a heterologous promoter containing LexA binding sites. [2] [5]





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References

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  1. 1.0 1.1 Kuchin S, et al. (2002) Snf1 protein kinase and the repressors Nrg1 and Nrg2 regulate FLO11, haploid invasive growth, and diploid pseudohyphal differentiation. Mol Cell Biol 22(12):3994-4000 SGD PMID 12024013
  2. 2.0 2.1 2.2 2.3 2.4 2.5 Vyas VK, et al. (2001) Interaction of the repressors Nrg1 and Nrg2 with the Snf1 protein kinase in Saccharomyces cerevisiae. Genetics 158(2):563-72 SGD PMID 11404322 Cite error: Invalid <ref> tag; name "S000060795" defined multiple times with different content Cite error: Invalid <ref> tag; name "S000060795" defined multiple times with different content Cite error: Invalid <ref> tag; name "S000060795" defined multiple times with different content Cite error: Invalid <ref> tag; name "S000060795" defined multiple times with different content Cite error: Invalid <ref> tag; name "S000060795" defined multiple times with different content
  3. 3.0 3.1 3.2 3.3 Vyas VK, et al. (2003) Snf1 kinases with different beta-subunit isoforms play distinct roles in regulating haploid invasive growth. Mol Cell Biol 23(4):1341-8 SGD PMID 12556493
  4. 4.0 4.1 4.2 4.3 submitted by Valmik K. Vyas on 2003-03-27
  5. 5.0 5.1 5.2 5.3 5.4 5.5 submitted by Valmik K. Vyas on 2003-02-04

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