YBR066C
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| Systematic name | YBR066C |
| Gene name | NRG2 |
| Aliases | |
| Feature type | ORF, Verified |
| Coordinates | Chr II:370699..370037 |
| Primary SGDID | S000000270 |
Description of YBR066C: Transcriptional repressor that mediates glucose repression and negatively regulates filamentous growth; has similarity to Nrg1p[1][2]
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Contents
Community Commentary
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Alleles, Strains, and Phenotypes
Multiple Knockout Strains
Strain Background: Sigma1278b (Sigma 2000 series)
Genotype: MCY4708
Together with: NRG1, SIP1, GAL83, SIP2
Phenotype(s): Loss of function (Null)
Deletion of nrg2 partially suppresses gal83's defect in invasive growth, plastic adherance, and STA2-lacZ expression. [3] [4]
Strain Background: Sigma1278b (Sigma 2000 series)
Genotype: MCY4712
Together with: NRG1, SIP1, GAL83, SIP2
Phenotype(s): Loss of function (Null)
Deletion of nrg1 and nrg2 suppresses gal83's defect in invasive growth, plastic adherance, and STA2-lacZ expression, better than deletion of either nrg alone. [3] [4]
Strain Background: Sigma1278b (Sigma 2000 series)
Genotype: MCY4708
Together with: NRG1, SIP1, GAL83, SIP2
Phenotype(s): Loss of function (Null)
Deletion of nrg2 partially suppresses gal83's defect in invasive growth, plastic adherance, and STA2-lacZ expression. [3] [4]
Strain Background: Sigma1278b (Sigma 2000 series)
Genotype: MCY4712
Together with: NRG1, SIP1, GAL83, SIP2
Phenotype(s): Loss of function (Null)
Deletion of nrg1 and nrg2 suppresses gal83's defect in invasive growth, plastic adherance, and STA2-lacZ expression, better than deletion of either nrg alone. [3] [4]
Overexpression
Phenotype(s): Dominant, Gain of function
Overexpression of VP16-Nrg2 results in partial derepression of SUC2 in glucose grown cells. [2] [5]
Interactions
Genetic
YBR066C is a suppressor of Snf1
Strain Background: Sigma 1278b
Mutation type(s): complete deletion, complete deletion
Deletion of Nrg2 partially suppresses Snf1's defect in invasive growth, biofilm formation, FLO11 expression, and STA2 expression. [1] [5]
Physical
Physical interaction with Snf1
LexA-Snf1 coimmunoprecipitates HA3-Nrg2 [2] [5]
Physical interaction with Snf1
Snf1 coimmunoprecipitates with HA3-Nrg2 [2] [5]
Two Hybrid
Two Hybrid interaction with Snf1
Snf1 kinase domain interacts with Nrg2 [2] [5]
Protein Details
Protein Function/Process
LexA-Nrg2 represses transcription from a heterologous promoter containing LexA binding sites. [2] [5]
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References
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- ↑ 1.0 1.1 Kuchin S, et al. (2002) Snf1 protein kinase and the repressors Nrg1 and Nrg2 regulate FLO11, haploid invasive growth, and diploid pseudohyphal differentiation. Mol Cell Biol 22(12):3994-4000 SGD PMID 12024013
- ↑ 2.0 2.1 2.2 2.3 2.4 2.5 Vyas VK, et al. (2001) Interaction of the repressors Nrg1 and Nrg2 with the Snf1 protein kinase in Saccharomyces cerevisiae. Genetics 158(2):563-72
SGD PMID 11404322 Cite error: Invalid
<ref>tag; name "S000060795" defined multiple times with different content Cite error: Invalid<ref>tag; name "S000060795" defined multiple times with different content Cite error: Invalid<ref>tag; name "S000060795" defined multiple times with different content Cite error: Invalid<ref>tag; name "S000060795" defined multiple times with different content Cite error: Invalid<ref>tag; name "S000060795" defined multiple times with different content - ↑ 3.0 3.1 3.2 3.3 Vyas VK, et al. (2003) Snf1 kinases with different beta-subunit isoforms play distinct roles in regulating haploid invasive growth. Mol Cell Biol 23(4):1341-8 SGD PMID 12556493
- ↑ 4.0 4.1 4.2 4.3 submitted by Valmik K. Vyas on 2003-03-27
- ↑ 5.0 5.1 5.2 5.3 5.4 5.5 submitted by Valmik K. Vyas on 2003-02-04
See Help:Categories on how to add the wiki page for this gene to a Category </protect>
- Pages with reference errors
- ORF
- ORF, Verified
- Topic:Alleles, Strains, and Phenotypes
- Topic:Alleles, Strains, and Phenotypes:Multiple Knockout Strains
- Phenotype:Loss of function (Null)
- Topic:Alleles, Strains, and Phenotypes:Overexpression
- Phenotype:Dominant
- Phenotype:Gain of function
- Topic:Interactions
- Topic:Interactions:Genetic
- Genetic Interaction with Snf1
- Topic:Interactions:Physical
- Physical Interactions with Snf1
- Topic:Interactions:Two Hybrid
- Two Hybrid Interactions with Snf1
- Topic:Protein Details
- Topic:Protein Details:Protein Function/Process
