UW Stout/Proline FA19

Materials

• Histidine
• Leucine
• uracil
• CSM
• YBN
• Glucose
• Praline
• Ammonium Sulfate
• Water
• Corning COSTAR 96-Well Clear Flat-Bottom Assay Plate

Protocol

Part 1

1. Label two separate 250ml glass media jars with Proline and Ammonium Sulfate
2. In the Proline jar mix the following materials

• Histidine 100ml
• Leucine 100ml
• Uracil 100ml
• CSM 0.065g
• YBN 0.171g
• Proline 0.1g
• Fill up to 250ml with water

1. In the Ammonium Sulfate jar mix the following materials

• Histidine 100ml
• Leucine 100ml
• Uracil 100ml
• CSM 0.065g
• YBN 0.171g
• Ammonium Sulfate 0.5g
• Fill up to 250ml with water

1. Mix well
2. Autoclave at 120 degrees Celsius for 15 minutes
3. Mix 5ml of glucose to both solutions