Difference between revisions of "UW Stout/Proline FA19"
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*Corning COSTAR 96-Well Clear Flat-Bottom Assay Plate | *Corning COSTAR 96-Well Clear Flat-Bottom Assay Plate | ||
==Protocol== | ==Protocol== | ||
| + | ===Part 1=== | ||
| + | #Label two separate 250ml glass media jars with Proline and Ammonium Sulfate | ||
| + | #In the Proline jar mix the following materials | ||
| + | *Histidine 100ml | ||
| + | *Leucine 100ml | ||
| + | *Uracil 100ml | ||
| + | *CSM 0.065g | ||
| + | *YBN 0.171g | ||
| + | *Proline 0.1g | ||
| + | *Fill up to 250ml with water | ||
| + | #In the Ammonium Sulfate jar mix the following materials | ||
| + | *Histidine 100ml | ||
| + | *Leucine 100ml | ||
| + | *Uracil 100ml | ||
| + | *CSM 0.065g | ||
| + | *YBN 0.171g | ||
| + | *Ammonium Sulfate 0.5g | ||
| + | *Fill up to 250ml with water | ||
| + | #Mix well | ||
| + | #Autoclave at 120 degrees Celsius for 15 minutes | ||
| + | #Mix 5ml of glucose to both solutions | ||
Revision as of 15:27, 15 December 2019
Materials
- Histidine
- Leucine
- uracil
- CSM
- YBN
- Glucose
- Praline
- Ammonium Sulfate
- Water
- Corning COSTAR 96-Well Clear Flat-Bottom Assay Plate
Protocol
Part 1
- Label two separate 250ml glass media jars with Proline and Ammonium Sulfate
- In the Proline jar mix the following materials
- Histidine 100ml
- Leucine 100ml
- Uracil 100ml
- CSM 0.065g
- YBN 0.171g
- Proline 0.1g
- Fill up to 250ml with water
- In the Ammonium Sulfate jar mix the following materials
- Histidine 100ml
- Leucine 100ml
- Uracil 100ml
- CSM 0.065g
- YBN 0.171g
- Ammonium Sulfate 0.5g
- Fill up to 250ml with water
- Mix well
- Autoclave at 120 degrees Celsius for 15 minutes
- Mix 5ml of glucose to both solutions
