UW-Stout/Nitrogen Starvation FA21
Contents
Nitrogen Starvation Experiment
Introduction
The purpose of this experiment is to test different variations of yeast and how they adapt to the pressure of starving them of nitrogen which is considered necessary for there growth and how the yeast react alterations in media. And if any strains of the yeast react better or worse to starvation and if they are able to grow efficiently without a nitrogen source available.
Protocol
Materials/equipment
- 15 plates of media that contains ammonium (nitrogen source)
- 15 plates of media that does not contain ammonium (no nitrogen source)
- 96 well plate
- Micro-pipettors
- Specialized multi-channel micro-pipette
- Micro pipette tips
- 6 PCR tubes containing wild yeast and 5 knockout strains
- 10-15ul of each yeast strain
- Sterile Water
- YNB
- CSM
- His
- Leu
- Ura
- Agar
- H2O
- Ammonium Source
