UW-Stout/Knockout Protocol

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Background strain: BY4735. BY4735 is a MATalpha 6-way auxotroph based on S288C, bearing non-revertable deletions in ADE2, HIS3, LEU2, MET15, TRP1 and URA3.
Plasmids: Knock-out plasmids were built using the Dueber lab's yeast toolkit. Additional assembly details are below.

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