UW-Stout/Knockout Protocol

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Resources

BY4735 is a [https://www.ncbi.nlm.nih.gov/pubmed/9483801 MATalpha 6-way auxotroph based on S288C], bearing non-revertable deletions in ADE2, HIS3, LEU2, MET15, TRP1 and URA3.

  • Plasmids: Knock-out plasmids were built using the

Dueber lab's yeast toolkit. Additional assembly details are below.

Retrieved from "https://wiki.yeastgenome.org/index.php?title=UW-Stout/Knockout_Protocol&oldid=400412"