Difference between revisions of "UW-Stout/Knockout Protocol"
(Created page with "==Resources== * Background strain: [https://www.atcc.org/en/Products/All/200897.aspx BY4735]. BY4735 is a [https://www.ncbi.nlm.nih.gov/pubmed/9483801 MATalpha 6-way auxotr...") |
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==Resources== | ==Resources== | ||
| − | * Background strain: [https://www.atcc.org/en/Products/All/200897.aspx BY4735]. | + | * Background strain: [https://www.atcc.org/en/Products/All/200897.aspx BY4735]. BY4735 is a [https://www.ncbi.nlm.nih.gov/pubmed/9483801 MATalpha 6-way auxotroph based on S288C], bearing non-revertable deletions in [[ADE2]], [[HIS3]], [[LEU2]], [[MET15]], [[TRP1]] and [[URA3]]. |
| − | BY4735 is a [https://www.ncbi.nlm.nih.gov/pubmed/9483801 MATalpha 6-way auxotroph | + | * Plasmids: Knock-out plasmids were built using the [https://pubs.acs.org/doi/10.1021/sb500366v Dueber lab's yeast toolkit.] Additional assembly details are below. |
| − | based on S288C], bearing non-revertable deletions in [[ADE2]], [[HIS3]], [[LEU2]], | ||
| − | [[MET15]], [[TRP1]] and [[URA3]]. | ||
| − | |||
| − | * Plasmids: Knock-out plasmids were built using the | ||
| − | [https://pubs.acs.org/doi/10.1021/sb500366v Dueber lab's yeast toolkit.] Additional | ||
| − | assembly details are below. | ||
Revision as of 11:47, 29 April 2019
Resources
- Background strain: BY4735. BY4735 is a MATalpha 6-way auxotroph based on S288C, bearing non-revertable deletions in ADE2, HIS3, LEU2, MET15, TRP1 and URA3.
- Plasmids: Knock-out plasmids were built using the Dueber lab's yeast toolkit. Additional assembly details are below.
