YMR144W

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Revision as of 11:24, 2 May 2023 by Teagueb (talk | contribs) (Heat Shock)
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Systematic name YMR144W
Gene name
Aliases
Feature type ORF, Uncharacterized
Coordinates Chr XIII:553362..554390
Primary SGDID S000004752


Description of YMR144W: Putative protein of unknown function; localized to the nucleus; YMR144W is not an essential gene[1][2]




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Isopropanol

UW-Stout/Isopropanol_SP23

Picture10.jpg

Analysis

These results were not consistent as there are 3 very different results. The first trial was way off what it was supposed to be and therefore could have been contaminated or been an error in the pipetting process. The second trial was the most successful and gave the results that were expected. The third trial was good except for the region from about 8:00-11:20 where it spiked up and down. The last 2 had more growth than the control which could mean that the cell without the gene that was knocked out is able to withstand more alcohol.

Heat Shock

YMR144W Yeast Colonies at 40 degrees Celsius
Time (sec) Colonies
0 >100
15 92
45 86
75 100
105 84
135 81
Wild Type Yeast Colonies at 40 degrees Celsius
Time (sec) Colonies
0 >100
15 92
45 86
75 100
105 84
135 81

DKATrial1Yeast.jpeg

DKA2 YMR144W.jpg

The general trend of our data yielded a negative relationship between time of heat shock. It can be inferred that with a longer amount of time we the yeast cells could continue being killed off until all of them died.

Bases

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References

See Help:References on how to add references

  1. Giaever G, et al. (2002) Functional profiling of the Saccharomyces cerevisiae genome. Nature 418(6896):387-91 SGD PMID 12140549
  2. Kumar A, et al. (2002) Subcellular localization of the yeast proteome. Genes Dev 16(6):707-19 SGD PMID 11914276

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