UW-Stout/Knockout Protocol
Resources
- Background strain: BY4735.
BY4735 is a [https://www.ncbi.nlm.nih.gov/pubmed/9483801 MATalpha 6-way auxotroph based on S288C], bearing non-revertable deletions in ADE2, HIS3, LEU2, MET15, TRP1 and URA3.
- Plasmids: Knock-out plasmids were built using the
Dueber lab's yeast toolkit. Additional assembly details are below.