YJL187C
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| Systematic name | YJL187C | |
| Gene name | SWE1 | |
| Aliases | WEE1 | |
| Feature type | ORF, Verified | |
| Coordinates | Chr X:79261..76802 | |
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Description of YJL187C: Protein kinase that regulates the G2/M transition by inhibition of Cdc28p kinase activity; localizes to the nucleus and to the daughter side of the mother-bud neck; homolog of S. pombe Wee1p; potential Cdc28p substrate[1][2][3]
Alleles, Strains, and Phenotypes
Complete Deletion
Strain Background: W303
Genotype: SH24: swe1::URA3 bar1
Phenotype(s): Loss of function (Null), Premature entry into mitosis, Viable
Despite previous reports to the contrary, swe1 mutant cells enter mitosis prematurely at a reduced bud size. [4] [5]
Point Mutation
Strain Background: BF264-15D
Phenotype(s): Dominant, stabilized
Deletion or mutation of residues between Leu320 and Tyr332 stabilizes Swe1p. [6] [7]
Interactions
Other Interactions
Other Topic: NOT Regulatory
Other Interactions interaction with MET30
Despite previous reports to the contrary, Met30p is not required for Swe1p degradation [6] [7]
Other
Localization
Swe1p localization to the neck abolished by mutations in residues Leu320, Leu324, Phe327, Lys328,Leu331, or Tyr332 [6] [7]
Protein Details
Protein Modification
Modification(s): Phosphorylation
Identified as an efficient substrate of Clb2-Cdk1-as1 in a screen of a proteomic GST-fusion library. [1] [8]
Contents
Community Commentary
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Alleles, Strains, and Phenotypes
Complete Deletion
Strain Background: W303
Genotype: SH24: swe1::URA3 bar1
Phenotype(s): Loss of function (Null), Premature entry into mitosis, Viable
Despite previous reports to the contrary, swe1 mutant cells enter mitosis prematurely at a reduced bud size. [4] [5]
Point Mutation
Strain Background: BF264-15D
Phenotype(s): Dominant, stabilized
Deletion or mutation of residues between Leu320 and Tyr332 stabilizes Swe1p. [6] [7]
Interactions
Other Interactions
Other Topic: NOT Regulatory
Other Interactions interaction with MET30
Despite previous reports to the contrary, Met30p is not required for Swe1p degradation [6] [7]
Other
Localization
Swe1p localization to the neck abolished by mutations in residues Leu320, Leu324, Phe327, Lys328,Leu331, or Tyr332 [6] [7]
Protein Details
Protein Modification
Modification(s): Phosphorylation
Identified as an efficient substrate of Clb2-Cdk1-as1 in a screen of a proteomic GST-fusion library. [1] [8]
References
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- ↑ 1.0 1.1 1.2 Ubersax JA, et al. (2003) Targets of the cyclin-dependent kinase Cdk1. Nature 425(6960):859-64 SGD PMID 14574415
- ↑ Longtine MS, et al. (2000) Septin-dependent assembly of a cell cycle-regulatory module in Saccharomyces cerevisiae. Mol Cell Biol 20(11):4049-61 SGD PMID 10805747
- ↑ Booher RN, et al. (1993) Properties of Saccharomyces cerevisiae wee1 and its differential regulation of p34CDC28 in response to G1 and G2 cyclins. EMBO J 12(9):3417-26 SGD PMID 8253069
- ↑ 4.0 4.1 Harvey SL and Kellogg DR (2003) Conservation of mechanisms controlling entry into mitosis: budding yeast wee1 delays entry into mitosis and is required for cell size control. Curr Biol 13(4):264-75 SGD PMID 12593792
- ↑ 5.0 5.1 submitted by Stacy Harvey on 2005-06-08
- ↑ 6.0 6.1 6.2 6.3 6.4 6.5 McMillan JN, et al. (2002) Determinants of Swe1p degradation in Saccharomyces cerevisiae. Mol Biol Cell 13(10):3560-75 SGD PMID 12388757
- ↑ 7.0 7.1 7.2 7.3 7.4 7.5 submitted by Daniel Lew on 2003-03-03
- ↑ 8.0 8.1 submitted by Jeff Ubersax on 2004-01-27
See Help:Categories on how to add the wiki page for this gene to a Category
- ORF
- ORF, Verified
- Topic:Alleles, Strains, and Phenotypes
- Topic:Alleles, Strains, and Phenotypes:Complete Deletion
- Phenotype:Loss of function (Null)
- Phenotype:Premature entry into mitosis
- Phenotype:Viable
- Topic:Alleles, Strains, and Phenotypes:Point Mutation
- Phenotype:Dominant
- Phenotype:stabilized
- Topic:Interactions
- Topic:Interactions:Other Interactions
- Topic:NOT Regulatory
- Other Interactions Interactions with MET30
- Topic:Other
- Topic:Other:Localization
- Topic:Protein Details
- Topic:Protein Details:Protein Modification
- Modification:Phosphorylation
