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Systematic name YJL187C
Gene name SWE1
Aliases WEE1
Feature type ORF, Verified
Coordinates Chr X:79262..76803
Primary SGDID S000003723

Description of YJL187C: Protein kinase that regulates the G2/M transition by inhibition of Cdc28p kinase activity; localizes to the nucleus and to the daughter side of the mother-bud neck; homolog of S. pombe Wee1p; potential Cdc28p substrate[1][2][3]


Community Commentary

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Alleles, Strains, and Phenotypes

Complete Deletion

Strain Background: W303 (see detailed notes from Rodney Rothstein and Stephan Bartsch for the W303 strain used in the study)
Genotype: SH24: swe1::URA3 bar1
Phenotype(s): Loss of function (Null), Premature entry into mitosis, Viable

Despite previous reports to the contrary, swe1 mutant cells enter mitosis prematurely at a reduced bud size. [4] [5]

Point Mutation

Strain Background: BF264-15D
Phenotype(s): Dominant, stabilized

Deletion or mutation of residues between Leu320 and Tyr332 stabilizes Swe1p. [6] [7]


Other Interactions

Other Topic: NOT Regulatory Other Interactions interaction with MET30
Despite previous reports to the contrary, Met30p is not required for Swe1p degradation [6] [7]



Swe1p localization to the neck abolished by mutations in residues Leu320, Leu324, Phe327, Lys328,Leu331, or Tyr332 [6] [7]

Protein Details

Protein Modification

Modification(s): Phosphorylation

Identified as an efficient substrate of Clb2-Cdk1-as1 in a screen of a proteomic GST-fusion library. [3] [8]



See Help:References on how to add references

  1. Booher RN, et al. (1993) Properties of Saccharomyces cerevisiae wee1 and its differential regulation of p34CDC28 in response to G1 and G2 cyclins. EMBO J 12(9):3417-26 SGD PMID 8253069
  2. Longtine MS, et al. (2000) Septin-dependent assembly of a cell cycle-regulatory module in Saccharomyces cerevisiae. Mol Cell Biol 20(11):4049-61 SGD PMID 10805747
  3. 3.0 3.1 Ubersax JA, et al. (2003) Targets of the cyclin-dependent kinase Cdk1. Nature 425(6960):859-64 SGD PMID 14574415 Cite error: Invalid <ref> tag; name "S000074306" defined multiple times with different content
  4. Harvey SL and Kellogg DR (2003) Conservation of mechanisms controlling entry into mitosis: budding yeast wee1 delays entry into mitosis and is required for cell size control. Curr Biol 13(4):264-75 SGD PMID 12593792
  5. submitted by Stacy Harvey on 2005-06-08
  6. 6.0 6.1 6.2 McMillan JN, et al. (2002) Determinants of Swe1p degradation in Saccharomyces cerevisiae. Mol Biol Cell 13(10):3560-75 SGD PMID 12388757
  7. 7.0 7.1 7.2 submitted by Daniel Lew on 2003-03-03
  8. submitted by Jeff Ubersax on 2004-01-27

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