YBR066C

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Systematic name YBR066C
Gene name NRG2
Aliases
Feature type ORF, Verified
Coordinates Chr II:370697..370035
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Description of YBR066C: Transcriptional repressor that mediates glucose repression and negatively regulates filamentous growth; has similarity to Nrg1p[1][2]



Alleles, Strains, and Phenotypes

Multiple Knockout Strains

Strain Background: Sigma1278b (Sigma 2000 series)
Genotype: MCY4708
Together with: NRG1, SIP1, GAL83, SIP2
Phenotype(s): Loss of function (Null)

Deletion of nrg2 partially suppresses gal83's defect in invasive growth, plastic adherance, and STA2-lacZ expression. [3] [4]

Strain Background: Sigma1278b (Sigma 2000 series)
Genotype: MCY4712
Together with: NRG1, SIP1, GAL83, SIP2
Phenotype(s): Loss of function (Null)

Deletion of nrg1 and nrg2 suppresses gal83's defect in invasive growth, plastic adherance, and STA2-lacZ expression, better than deletion of either nrg alone. [3] [4]

Strain Background: Sigma1278b (Sigma 2000 series)
Genotype: MCY4708
Together with: NRG1, SIP1, GAL83, SIP2
Phenotype(s): Loss of function (Null)

Deletion of nrg2 partially suppresses gal83's defect in invasive growth, plastic adherance, and STA2-lacZ expression. [3] [4]

Strain Background: Sigma1278b (Sigma 2000 series)
Genotype: MCY4712
Together with: NRG1, SIP1, GAL83, SIP2
Phenotype(s): Loss of function (Null)

Deletion of nrg1 and nrg2 suppresses gal83's defect in invasive growth, plastic adherance, and STA2-lacZ expression, better than deletion of either nrg alone. [3] [4]

Overexpression

Phenotype(s): Dominant, Gain of function

Overexpression of VP16-Nrg2 results in partial derepression of SUC2 in glucose grown cells. [2] [5]

Interactions

Genetic

YBR066C is a suppressor of Snf1


Strain Background: Sigma 1278b
Mutation type(s): complete deletion, complete deletion

Deletion of Nrg2 partially suppresses Snf1's defect in invasive growth, biofilm formation, FLO11 expression, and STA2 expression. [1] [5]

Physical

Physical interaction with Snf1
LexA-Snf1 coimmunoprecipitates HA3-Nrg2 [2] [5]

Physical interaction with Snf1
Snf1 coimmunoprecipitates with HA3-Nrg2 [2] [5]

Two Hybrid

Two Hybrid interaction with Snf1
Snf1 kinase domain interacts with Nrg2 [2] [5]

Protein Details

Protein Function/Process

LexA-Nrg2 represses transcription from a heterologous promoter containing LexA binding sites. [2] [5]

Community Commentary

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Alleles, Strains, and Phenotypes

Multiple Knockout Strains

Strain Background: Sigma1278b (Sigma 2000 series)
Genotype: MCY4708
Together with: NRG1, SIP1, GAL83, SIP2
Phenotype(s): Loss of function (Null)

Deletion of nrg2 partially suppresses gal83's defect in invasive growth, plastic adherance, and STA2-lacZ expression. [3] [4]

Strain Background: Sigma1278b (Sigma 2000 series)
Genotype: MCY4712
Together with: NRG1, SIP1, GAL83, SIP2
Phenotype(s): Loss of function (Null)

Deletion of nrg1 and nrg2 suppresses gal83's defect in invasive growth, plastic adherance, and STA2-lacZ expression, better than deletion of either nrg alone. [3] [4]

Strain Background: Sigma1278b (Sigma 2000 series)
Genotype: MCY4708
Together with: NRG1, SIP1, GAL83, SIP2
Phenotype(s): Loss of function (Null)

Deletion of nrg2 partially suppresses gal83's defect in invasive growth, plastic adherance, and STA2-lacZ expression. [3] [4]

Strain Background: Sigma1278b (Sigma 2000 series)
Genotype: MCY4712
Together with: NRG1, SIP1, GAL83, SIP2
Phenotype(s): Loss of function (Null)

Deletion of nrg1 and nrg2 suppresses gal83's defect in invasive growth, plastic adherance, and STA2-lacZ expression, better than deletion of either nrg alone. [3] [4]

Overexpression

Phenotype(s): Dominant, Gain of function

Overexpression of VP16-Nrg2 results in partial derepression of SUC2 in glucose grown cells. [2] [5]

Interactions

Genetic

YBR066C is a suppressor of Snf1


Strain Background: Sigma 1278b
Mutation type(s): complete deletion, complete deletion

Deletion of Nrg2 partially suppresses Snf1's defect in invasive growth, biofilm formation, FLO11 expression, and STA2 expression. [1] [5]

Physical

Physical interaction with Snf1
LexA-Snf1 coimmunoprecipitates HA3-Nrg2 [2] [5]

Physical interaction with Snf1
Snf1 coimmunoprecipitates with HA3-Nrg2 [2] [5]

Two Hybrid

Two Hybrid interaction with Snf1
Snf1 kinase domain interacts with Nrg2 [2] [5]

Protein Details

Protein Function/Process

LexA-Nrg2 represses transcription from a heterologous promoter containing LexA binding sites. [2] [5]

References

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  1. ↑ Jump up to: 1.0 1.1 1.2 Kuchin S, et al. (2002) Snf1 protein kinase and the repressors Nrg1 and Nrg2 regulate FLO11, haploid invasive growth, and diploid pseudohyphal differentiation. Mol Cell Biol 22(12):3994-4000 SGD PMID 12024013
  2. ↑ Jump up to: 2.00 2.01 2.02 2.03 2.04 2.05 2.06 2.07 2.08 2.09 2.10 Vyas VK, et al. (2001) Interaction of the repressors Nrg1 and Nrg2 with the Snf1 protein kinase in Saccharomyces cerevisiae. Genetics 158(2):563-72 SGD PMID 11404322 Cite error: Invalid <ref> tag; name "S000060795" defined multiple times with different content Cite error: Invalid <ref> tag; name "S000060795" defined multiple times with different content Cite error: Invalid <ref> tag; name "S000060795" defined multiple times with different content Cite error: Invalid <ref> tag; name "S000060795" defined multiple times with different content Cite error: Invalid <ref> tag; name "S000060795" defined multiple times with different content Cite error: Invalid <ref> tag; name "S000060795" defined multiple times with different content Cite error: Invalid <ref> tag; name "S000060795" defined multiple times with different content Cite error: Invalid <ref> tag; name "S000060795" defined multiple times with different content Cite error: Invalid <ref> tag; name "S000060795" defined multiple times with different content Cite error: Invalid <ref> tag; name "S000060795" defined multiple times with different content
  3. ↑ Jump up to: 3.0 3.1 3.2 3.3 3.4 3.5 3.6 3.7 Vyas VK, et al. (2003) Snf1 kinases with different beta-subunit isoforms play distinct roles in regulating haploid invasive growth. Mol Cell Biol 23(4):1341-8 SGD PMID 12556493
  4. ↑ Jump up to: 4.0 4.1 4.2 4.3 4.4 4.5 4.6 4.7 submitted by Valmik K. Vyas on 2003-03-27
  5. ↑ Jump up to: 5.00 5.01 5.02 5.03 5.04 5.05 5.06 5.07 5.08 5.09 5.10 5.11 submitted by Valmik K. Vyas on 2003-02-04

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