YKR005C

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Systematic name	YKR005C
Gene name
Aliases
Feature type	ORF, Uncharacterized
Coordinates	Chr XI:450167..448521
Primary SGDID	S000001713

Description of YKR005C: Putative protein of unknown function




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Community Commentary

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This gene is part of the UW-Stout Orphan Gene Project. Learn more here.

Growth in YPD

In a BY4735 background, knocking out YKR005C seems to have a little to no effect on growth rate in log-phase. In this assay, the BY4735 strain's doubling time was 414 minutes, while the YKR005C knock-out strain's doubling time was 427 minutes. (These doubling times are the means of three experiments.)

Caffeine and Yeast Cells

This graph shows the growth curve with and without caffeine. It has the knockout strain and wild type yeast cells. The caffeine enhanced the growth in both wild type and knockout strain. The average doubling time between the three experiments for the knockout strand was 736.38 minutes. The caffeine also had a positive effect on the growth curve for the wild type with the average doubling time being 1245.02 minutes. The results were concluded from the wild type and knockout strand was with this amount of caffeine it leads to an increase in growth.

The protocol can be found at

Caffeine

This bar graph shows that compared to a BY4735 background, Knocking out yKR005c makes yeast a little more sensitive to UV light.

the protocol can be found at

Cycloheximide effects on YKR005C

As we can see from the image above Tests 1-3 are below the growth curve and did not function as well. Test 4 is above which is weird. The growth curve is made up of the growth curve models 1-3 which are not being stressed.

Effect of 3% DMSO on YKR005C

The graph above shows the effect of 3% DMSO on YKR005C. While examining the data from test 3 it is thought that the 3% DMSO test on YKR005C was contaminated; the results are still shown on the graph but the information was not used during growth rate calculations. From the data represented the average doubling rate for the YKR005C knockout strain without DMSO was 519 minutes. When DMSO is added the average doubling time increased to 1490 minutes. This shows that 3% DMSO did add stress to this knockout strain. The average doubling time of WT:BY4735 without DMSO was 514 minutes. The average doubling rate for WT:BY4735 with DMSO was 1529 minutes. If we compare the average doubling rate of WT:BY4735 with DMSO to YKR005C with DMSO we see the yeast grew at about the same rate under DMSO induced stress which means that the YKR005C had very minimal effects on growth rate under stress. It is also observed that both control groups had a growth rate within 5 minutes of each other, which means that the YKR005C gene has no effect of yeast growth in optimal conditions.

Ethanol Tolerance in YKR005C

This graph shows the growth between YKR005C treated and untreated with ethanol. The rate of growth of the yeast treated with ethanol is (answer). The rate of growth of the yeast not treated with ethanol is (answer). This shows that the strands of YKR005C treated with ethanol had an increased growth rate compared to those that weren't.

Prednisone Effects on YKR005C

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References

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