YKL121W
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| Systematic name | YKL121W |
| Gene name | DGR2 |
| Aliases | |
| Feature type | ORF, Uncharacterized |
| Coordinates | Chr XI:214142..216700 |
| Primary SGDID | S000001604 |
Description of YKL121W: Protein of unknown function; null mutant is resistant to 2-deoxy-D-glucose and displays abnormally elongated buds[1][2][3]
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Contents
Community Commentary
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This gene is part of the UW-Stout Orphan Gene Project. Learn more here.
Growth Curve
In a BY4735 background, knocking out YKL121W seems to substantially slow the strain's growth. In this assay, the BY4735 strain's doubling time was 162 minutes, while the YKL121W knock-out strain's doubling time was 235 minutes. (These doubling times are the means of three experiments.)
Hydroxychloroquine
HCQ refers to hydroxychloroquine
Under normal conditions, the BY4753 doubling time is 144 minutes, while that of YKL121W is 139 minutes. This proves that both of these strains grow at a similar rate. When grown in an environment with hydroxychloroquine, YKL121W doubled in 763 minutes, while BY4753 doubled in 276 minutes. Both strains are inhibited by hydroxychloroquine, but YKL121W growth is staggered much more. This is a possible indicator that the knocked-out gene was involved in cellular protection from hydroxychloroquine. (These doubling times and curves are the means of three experiments.)
Salt Concentration (NaCl)
0mM NaCl conc. BY4735 strain's doubling time: 169 minutes
0mM NaCl conc. YKL121W strain's doubling time: 230 minutes
750mM NaCl conc. BY4735 strain's doubling time: 294 minutes
750mM NaCl conc. YKL121W strain's doubling time: 450 minutes
The graph above shows the growth rate for the previously listed strains and the relative level of NaCl concentration. Knocking out the gene negatively impacted the growth rate. Comparing the YKL121W doubling times in 0mM and 750mM NaCl, there is a tremendous effect on the growth rate. This effect negatively impacts the knockout strain's (YKL121W) growth rate.
Caffeine Group 1
Caffeine had an effect on the YKL121W strain. The doubling times are as followed:
Doubling time of YKL121W with caffeine: 406 minutes
Doubling time of YKL121W without caffeine: 358 minutes
Doubling time of BY4735 with caffeine: -2649 minutes
Doubling time of BY4735 without caffeine: 187 minutes
According to the graph, the knockout strain's growth rate had significantly slowed down when caffeine was added. <protect>
References
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- ↑ Colleaux L, et al. (1992) Sequence of a segment of yeast chromosome XI identifies a new mitochondrial carrier, a new member of the G protein family, and a protein with the PAAKK motif of the H1 histones. Yeast 8(4):325-36 SGD PMID 1514329
- ↑ Ralser M, et al. (2008) A catabolic block does not sufficiently explain how 2-deoxy-D-glucose inhibits cell growth. Proc Natl Acad Sci U S A 105(46):17807-17811 SGD PMID 19004802
- ↑ Watanabe M, et al. (2009) Comprehensive and quantitative analysis of yeast deletion mutants defective in apical and isotropic bud growth. Curr Genet 55(4):365-80 SGD PMID 19466415
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