YKR005C

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Systematic name YKR005C
Gene name
Aliases
Feature type ORF, Uncharacterized
Coordinates Chr XI:450167..448521
Primary SGDID S000001713


Description of YKR005C: Putative protein of unknown function[1]




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Community Commentary

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This gene is part of the UW-Stout Orphan Gene Project. Learn more here.

Growth in YPD

YKR005C YPD.png

In a BY4735 background, knocking out YKR005C seems to have a little to no effect on growth rate in log-phase. In this assay, the BY4735 strain's doubling time was 414 minutes, while the YKR005C knock-out strain's doubling time was 427 minutes. (These doubling times are the means of three experiments.)

Caffeine and Yeast Cells

megan29.png

This graph shows the growth curve with and without caffeine. It has the knockout strain and wild type yeast cells. The caffeine enhanced the growth in both wild type and knockout strain. The average doubling time between the three experiments for the knockout strand was 736.38 minutes. The caffeine also had a positive effect on the growth curve for the wild type with the average doubling time being 1245.02 minutes. The results were concluded from the wild type and knockout strand was with this amount of caffeine it leads to an increase in growth.

The protocol can be found at

Caffeine

yKR005cUV Exposure.png

This bar graph shows that compared to a BY4735 background, Knocking out yKR005c makes yeast a little more sensitive to UV light.

the protocol can be found at

UV Light Exposure

Ethanol Tolerance in YKR005C

YKR005C ethonal.png

This graph shows the growth between YKR005C treated and untreated with ethanol. The rate of growth of the yeast treated with ethanol is (answer). The rate of growth of the yeast not treated with ethanol is (answer). This shows that the strands of YKR005C treated with ethanol had an increased growth rate compared to those that weren't.

Prednisone Effects on YKR005C

YKR005C.png



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References

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  1. ↑ Dusterhoft A and Philippsen P (1992) DNA sequencing and analysis of a 24.7 kb segment encompassing centromere CEN11 of Saccharomyces cerevisiae reveals nine previously unknown open reading frames. Yeast 8(9):749-59 SGD PMID 1441752

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