Difference between revisions of "YMR144W"
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==Isopropanol== | ==Isopropanol== | ||
Revision as of 12:21, 2 May 2023
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| Systematic name | YMR144W |
| Gene name | |
| Aliases | |
| Feature type | ORF, Uncharacterized |
| Coordinates | Chr XIII:553362..554390 |
| Primary SGDID | S000004752 |
Description of YMR144W: Putative protein of unknown function; localized to the nucleus; YMR144W is not an essential gene[1][2]
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Contents
Acids
Analysis
Isopropanol
Analysis
These results were not consistent as there are 3 very different results. The first trial was way off what it was supposed to be and therefore could have been contaminated or been an error in the pipetting process. The second trial was the most successful and gave the results that were expected. The third trial was good except for the region from about 8:00-11:20 where it spiked up and down. The last 2 had more growth than the control which could mean that the cell without the gene that was knocked out is able to withstand more alcohol.
Glucose Sensitivity
[1]Protocol
Analysis
The table above shows the number of cells with YMR144W removed that were counted as dead out of 100 counted cells. We found from our data that there was approximately a 15% decrease in the cell population. It was surprising to find that by removing this gene there was an increase in cell viability. Further research may be required for more conclusive results.
Heat Shock
| Time (sec) | Colonies | |
|---|---|---|
| 0 | >100 | |
| 15 | 92 | |
| 45 | 86 | |
| 75 | 100 | |
| 105 | 84 | |
| 135 | 81 |
| Time (sec) | Colonies | |
|---|---|---|
| 0 | 24 | |
| 15 | 20 | |
| 45 | 17 | |
| 75 | 16 | |
| 105 | 18 | |
| 135 | 14 |
Analysis
The general trend of our data yielded a negative relationship between time of heat shock. It can be inferred that with a longer amount of time we the yeast cells could continue being killed off until all of them died. Only a few of the YMR144W yeast cells died off in a matter of 135 seconds at 40 degrees Celsius. The Wild Type yeast cells were dying off at a faster rate over time compared to the YMR144W strain. This means that the gene that was knocked out in the YMR144W yeast is more senstitive to heat shock, because without that gene it is more resistant to the heat shock.
Bases
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Analysis Yeast 1 showed the best growth trend in the basic environment with the data over the 24 hour reading period. This gene could potentially aid basic environmental resistance.
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References
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