Difference between revisions of "YNL018C"
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Revision as of 11:09, 27 April 2021
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| Systematic name | YNL018C |
| Gene name | |
| Aliases | |
| Feature type | ORF, Uncharacterized |
| Coordinates | Chr XIV:601774..599936 |
| Primary SGDID | S000004963 |
Description of YNL018C: Putative protein of unknown function[1]
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Contents
Community Commentary
About Community Commentary. Please share your knowledge!
This gene is part of the UW-Stout Orphan Gene Project. Learn more here.
Growth Curve
In a BY4735 background, knocking out YNL018C seems to have a modest effect on growth rate, if any. In this assay, the BY4735 strain's doubling time was 162 minutes, while the YNL018C knock-out strain's doubling time was 129 minutes. (These doubling times are the means of three experiments.)
Hydroxychloroquine
HCQ refers to hydroxychloroquine
Under normal conditions, the BY4753 doubling time is 144 minutes, while that of YNL018C is 145 minutes. This proves that both of these strains grow at a similar rate. When grown in an environment with hydroxychloroquine, YNL018C doubled in 211 minutes, while BY4753 doubled in 276 minutes. Both strains are inhibited by hydroxychloroquine, but BY4753 growth is staggered more. In hydroxychloroquine, BY4753 has a longer lag phase than that of YNL018C. (These doubling times and curves are the means of three experiments.)
Salt Concentration (NaCl)
0mM NaCl conc. BY4735 strain's doubling time: 169 minutes
0mM NaCl conc. YNL018C strain's doubling time: 128 minutes
750mM NaCl conc. BY4735 strain's doubling time: 294 minutes
750mM NaCl conc. YNL018C strain's doubling time: 195 minutes
The graph above shows the growth rate for the previously listed strains and the relative level of NaCl concentration. Knocking out the gene positively influences the growth rate. Comparing the YNL018C doubling times between 0mM and 750mM NaCl, there is a moderate effect on the growth rate. This effect negatively influences the knockout strain's (YNL018C) growth rate.
Caffeine Group 1
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References
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