Difference between revisions of "YEL035C"
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| + | ===[[UW-Stout/Sensitivity To Nitrogen Starvation]]=== | ||
| + | [[File: YEL035_growth_plate.JPG|thumb|Growth of YEL035C on synthetic defined media (SDC).]] | ||
| + | [[File: YEL035C_starvation plate.JPG|thumb|Growth of YELO35C on omission media that lacks nitrogen.]] | ||
| + | [[File: YEL035C_revival plate.JPG|thumb|Growth of transferred BY4735 on Synthetic defined media.]] | ||
| + | Knocking out the YGL035C seems to have no effect on growth after incubating for 5 days on the Nitrogen omitted media. | ||
===[[UW-Stout/Ultraviolet Light|Ultraviolet Sensitivity]]=== | ===[[UW-Stout/Ultraviolet Light|Ultraviolet Sensitivity]]=== | ||
Revision as of 06:37, 2 May 2019
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| Systematic name | YEL035C |
| Gene name | UTR5 |
| Aliases | |
| Feature type | ORF, Uncharacterized |
| Coordinates | Chr V:85545..85045 |
| Primary SGDID | S000000761 |
Description of YEL035C: Protein of unknown function; transcription may be regulated by Gcr1p; essential for growth under standard (aerobic) conditions but not under anaerobic conditions[1][2]
Contents
Community Commentary
About Community Commentary. Please share your knowledge!
This gene is part of the UW-Stout Orphan Gene Project. Learn more here.
UW-Stout/Sensitivity To Nitrogen Starvation
Knocking out the YGL035C seems to have no effect on growth after incubating for 5 days on the Nitrogen omitted media.
Ultraviolet Sensitivity
_uv_light.jpg)
YEL035C is more sensitive than BY4735(wild) given that there is less colonizes present when under the same stress.
Growth Rate in YPD
In a BY4735 background, knocking out YEL035C seems to have a moderate effect on growth rate in log-phase in YPD media. In this assay, the BY4735 strain's doubling time was 124 minutes, while the YEL035C knock-out strain's doubling time was 220 minutes.
G-418 Stress
Methanol sensitivity
The wild type strain had a little difference in the doubling time when we added methanol, but the methanol killed all of the cells in the YEL035c strain. [[1]]
References
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- ↑ Lopez MC and Baker HV (2000) Understanding the growth phenotype of the yeast gcr1 mutant in terms of global genomic expression patterns. J Bacteriol 182(17):4970-8 SGD PMID 10940042
- ↑ Snoek IS and Steensma HY (2006) Why does Kluyveromyces lactis not grow under anaerobic conditions? Comparison of essential anaerobic genes of Saccharomyces cerevisiae with the Kluyveromyces lactis genome. FEMS Yeast Res 6(3):393-403 SGD PMID 16630279
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