Difference between revisions of "YBR102C"

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Specifically higher expression in carbon limited chemostat cultures versus carbon excess.
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<ref>Boer VM, et al. (2003) The genome-wide transcriptional responses of Saccharomyces cerevisiae grown on glucose in aerobic chemostat cultures limited for carbon, nitrogen, phosphorus, or sulfur.
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J Biol Chem 278(5):3265-74</ref>
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Revision as of 12:02, 21 February 2007

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Systematic name YBR102C
Gene name EXO84
Aliases USA3
Feature type ORF, Verified
Coordinates Chr II:447317..445056


Description of YBR102C: Essential protein with dual roles in spliceosome assembly and exocytosis; the exocyst complex (Sec3p, Sec5p, Sec6p, Sec8p, Sec10p, Sec15p, Exo70p, and Exo84p) mediates polarized targeting of secretory vesicles to active sites of exocytosis[1][2][3]




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Interactions

Two Hybrid

Two Hybrid interaction with Prp8
Prp8 binds to C-terminus of Exo84 [4] [5]


Protein Details

Protein Modification

Modification(s): Phosphorylation

Identified as an efficient substrate of Clb2-Cdk1-as1 in a screen of a proteomic GST-fusion library. [1] [6]




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References

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  1. 1.0 1.1 Ubersax JA, et al. (2003) Targets of the cyclin-dependent kinase Cdk1. Nature 425(6960):859-64 SGD PMID 14574415
  2. Awasthi S, et al. (2001) New roles for the Snp1 and Exo84 proteins in yeast pre-mRNA splicing. J Biol Chem 276(33):31004-15 SGD PMID 11425851
  3. Guo W, et al. (1999) Exo84p is an exocyst protein essential for secretion. J Biol Chem 274(33):23558-64 SGD PMID 10438536
  4. Kuhn AN and Brow DA (2000) Suppressors of a cold-sensitive mutation in yeast U4 RNA define five domains in the splicing factor Prp8 that influence spliceosome activation. Genetics 155(4):1667-82 SGD PMID 10924465
  5. submitted by Richard Grainger on 2003-04-11
  6. submitted by Jeff Ubersax on 2004-01-21

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