Difference between revisions of "YKR005C"
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the protocol can be found at | the protocol can be found at | ||
===[[UW_Stout/UV_Light_FA19|UV Light Exposure]]=== | ===[[UW_Stout/UV_Light_FA19|UV Light Exposure]]=== | ||
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| + | ===Cycloheximide effects on YKR005C=== | ||
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| + | [[File:Cycloheximide 11.png]] | ||
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| + | As we can see from the image above Tests 1-3 are below the growth curve and did not function as well. Test 4 is above which is weird. The growth curve is made up of the growth curve models 1-3 which are not being stressed. | ||
==Ethanol Tolerance in YKR005C== | ==Ethanol Tolerance in YKR005C== | ||
Revision as of 14:55, 12 December 2019
Share your knowledge...Edit this entry! <protect>
| Systematic name | YKR005C |
| Gene name | |
| Aliases | |
| Feature type | ORF, Uncharacterized |
| Coordinates | Chr XI:450167..448521 |
| Primary SGDID | S000001713 |
Description of YKR005C: Putative protein of unknown function[1]
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Contents
Community Commentary
About Community Commentary. Please share your knowledge!
This gene is part of the UW-Stout Orphan Gene Project. Learn more here.
Growth in YPD
In a BY4735 background, knocking out YKR005C seems to have a little to no effect on growth rate in log-phase. In this assay, the BY4735 strain's doubling time was 414 minutes, while the YKR005C knock-out strain's doubling time was 427 minutes. (These doubling times are the means of three experiments.)
Caffeine and Yeast Cells
This graph shows the growth curve with and without caffeine. It has the knockout strain and wild type yeast cells. The caffeine enhanced the growth in both wild type and knockout strain. The average doubling time between the three experiments for the knockout strand was 736.38 minutes. The caffeine also had a positive effect on the growth curve for the wild type with the average doubling time being 1245.02 minutes. The results were concluded from the wild type and knockout strand was with this amount of caffeine it leads to an increase in growth.
The protocol can be found at
Caffeine
This bar graph shows that compared to a BY4735 background, Knocking out yKR005c makes yeast a little more sensitive to UV light.
the protocol can be found at
UV Light Exposure
Cycloheximide effects on YKR005C
As we can see from the image above Tests 1-3 are below the growth curve and did not function as well. Test 4 is above which is weird. The growth curve is made up of the growth curve models 1-3 which are not being stressed.
Ethanol Tolerance in YKR005C
This graph shows the growth between YKR005C treated and untreated with ethanol. The rate of growth of the yeast treated with ethanol is (answer). The rate of growth of the yeast not treated with ethanol is (answer). This shows that the strands of YKR005C treated with ethanol had an increased growth rate compared to those that weren't.
Prednisone Effects on YKR005C
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References
See Help:References on how to add references
See Help:Categories on how to add the wiki page for this gene to a Category </protect>
