Difference between revisions of "YBR138C"
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The protocol can be found at | The protocol can be found at | ||
===[[UW_Stout/UV_Light_FA19|UV Light Exposure]]=== | ===[[UW_Stout/UV_Light_FA19|UV Light Exposure]]=== | ||
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| + | ===Cycloheximide effecting YBR138C=== | ||
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| + | [[File:Cycloheximide 5.png]] | ||
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| + | In this image we see that tests 2&3 are surviving and tests 1&4 are not doing as well under this stress. 1 is very low compared to 3 and this is kind of weird. The three Growth models are YBR138C not be stressed. | ||
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==Ethanol Tolerance in YBR138C== | ==Ethanol Tolerance in YBR138C== | ||
[[Image:YBR138C_ethonal.png]] | [[Image:YBR138C_ethonal.png]] | ||
Revision as of 14:20, 12 December 2019
Share your knowledge...Edit this entry! <protect>
| Systematic name | YBR138C |
| Gene name | |
| Aliases | HDR1 |
| Feature type | ORF, Uncharacterized |
| Coordinates | Chr II:515336..513762 |
| Primary SGDID | S000000342 |
Description of YBR138C: Cytoplasmic protein of unknown function, potentially phosphorylated by Cdc28p; YBR138C is not an essential gene[1][2][3]
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Contents
Community Commentary
About Community Commentary. Please share your knowledge!
Protein Details
Protein Modification
Modification(s): Phosphorylation
Identified as an efficient substrate of Clb2-Cdk1-as1 in a screen of a proteomic GST-fusion library. [3] [4]
This gene is part of the UW-Stout Orphan Gene Project. Learn more here.
Growth Curve
In a BY4735 background, knocking out YBR138C seems to have no effect on growth rate in log-phase. In this assay, the BY4735 strain's doubling time was 414 minutes, while the YBR138C knock-out strain's doubling time was 432minutes. (These doubling times are the means of three experiments.)
Caffeine and Yeast Cells
This graph shows the growth curve with and without caffeine. It has the knockout strain and wild type yeast cells. The caffeine enhanced the growth in both wild type and knockout strain. The average doubling time between the three experiments for the knockout strand was 689.46 minutes. The caffeine also had a positive effect on the growth curve for the wild type with the average doubling time being 1245.02 minutes. The results were concluded from the wild type and knockout strand was with this amount of caffeine it leads to an increase in growth.
The protocol can be found at
Caffeine
This bar graph shows that compared to a BY4735 background, yBR138c makes yeast less sensitive to UV light exposure.
The protocol can be found at
UV Light Exposure
Cycloheximide effecting YBR138C
In this image we see that tests 2&3 are surviving and tests 1&4 are not doing as well under this stress. 1 is very low compared to 3 and this is kind of weird. The three Growth models are YBR138C not be stressed.
Ethanol Tolerance in YBR138C
This graph shows the growth between YBR138C treated and untreated with ethanol. The rate of growth of the treated yeast is (answer). The rate of growth of the untreated yeast is (answer). This shows that the strands of YBR138C treated with ethanol had an increased rate of growth compared to the strands that weren't.
Prednisone Effects on YBR138C
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References
See Help:References on how to add references
- ↑ Huh WK, et al. (2003) Global analysis of protein localization in budding yeast. Nature 425(6959):686-91 SGD PMID 14562095
- ↑ Kucharczyk R, et al. (1999) Disruption of six novel yeast genes located on chromosome II reveals one gene essential for vegetative growth and two required for sporulation and conferring hypersensitivity to various chemicals. Yeast 15(10B):987-1000 SGD PMID 10407278
- ↑ 3.0 3.1 Ubersax JA, et al. (2003) Targets of the cyclin-dependent kinase Cdk1. Nature 425(6960):859-64
SGD PMID 14574415 Cite error: Invalid
<ref>tag; name "S000074306" defined multiple times with different content - ↑ submitted by Jeff Ubersax on 2004-01-21
See Help:Categories on how to add the wiki page for this gene to a Category </protect>
