Difference between revisions of "YCL002C"

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(Community Commentary)
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===[[UW-Stout/Sensitivity To Nitrogen Starvation]]===
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[[File: YCLO02C_growth plate.JPG|thumb|Growth of YCLO02C on synthetic defined media (SDC).]]
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[[File: YCL002C starvation plate1.JPG|thumb|Growth of YCLO02C on omission media that lacks nitrogen.]]
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Knocking out the YCLO02C seems to have no effect on growth after incubating for 5 days on the Nitrogen omitted media.
  
 
===[[UW-Stout/Ultraviolet Light|Ultraviolet Sensitivity]]===
 
===[[UW-Stout/Ultraviolet Light|Ultraviolet Sensitivity]]===

Revision as of 06:37, 2 May 2019

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Systematic name YCL002C
Gene name
Aliases
Feature type ORF, Uncharacterized
Coordinates Chr III:111675..110808
Primary SGDID S000000508


Description of YCL002C: Putative protein of unknown function; YCL002C is not an essential gene[1]




Community Commentary

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UW-Stout/Sensitivity To Nitrogen Starvation

Growth of YCLO02C on synthetic defined media (SDC).
Growth of YCLO02C on omission media that lacks nitrogen.

Knocking out the YCLO02C seems to have no effect on growth after incubating for 5 days on the Nitrogen omitted media.

Ultraviolet Sensitivity

YCL002C uv light.jpg.jpg BY4735(wild) uv light.jpg

YCL002C is more sensitive than BY4735(wild) given that there is less colonizes present when under the same stress.


This gene is part of the UW-Stout Orphan Gene Project. Learn more here.

Growth Rate in YPD

ycl002c growth.png

In a BY4735 background, knocking out YCL002C seems to have no effect on growth rate in log-phase in YPD. In this assay, the BY4735 strain's doubling time was 124 minutes, while the YCL002C knock-out strain's doubling time was 134 minutes. However, the knock-out strain did reach a substantially higher OD600 before saturating.

image001.png

Methanol Sensitivity

The wild type had a doubling time of 175 minutes, the YCL002 had a doubling time of 315 minutes. The addition of methanol sped up our reaction and caused the yeast cells to multiply faster.

[1]

YCL002C Methanol.png



References

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  1. Giaever G, et al. (2002) Functional profiling of the Saccharomyces cerevisiae genome. Nature 418(6896):387-91 SGD PMID 12140549

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