Difference between revisions of "YDL140C"
(→References) |
(→Community Commentary) |
||
| Line 30: | Line 30: | ||
{{CommentaryHelp}} | {{CommentaryHelp}} | ||
| + | Rpb1-1 mutation: this is a ts mutant which does not transcribe at the restrictive temperature. According to Scafe, S., Martin, C., Nonet, M., Podos, S., Okamura, S. and Young, R. (MCB 1990 vol. 10(3):1270-1275) this mutation is G->A at base 4622 (base 1 is A of ATG). However, according the Nedea, EC., Markov, D., Naryshkina, T. and Severinov, K. (J. Bact. 1999 Vol 181(8):2663-2665) the rpb1-1 mutation is equivalent to a parallel ts mutation in the E. coli RNA pol, in a conserved Glycine. According to their article, the rpb1-1 mutation is in this conserved Gly (aa 1437, or base 4310) and not Gly 1541 (base 4622 according to R. Young). | ||
| + | After sequencing two rpb1-1 mutants in our posession, we agree with Severinov, that the G->A mutation is at base 4310 and NOT in base 4622. | ||
| Line 43: | Line 45: | ||
<protect> | <protect> | ||
| + | |||
==References== | ==References== | ||
<!-- REFERENCES ARE AUTOMATICALLY GENERATED. PLEASE DON'T EDIT THIS SECTION--> | <!-- REFERENCES ARE AUTOMATICALLY GENERATED. PLEASE DON'T EDIT THIS SECTION--> | ||
{{RefHelp}} | {{RefHelp}} | ||
</protect> | </protect> | ||
Revision as of 00:06, 15 April 2008
Share your knowledge...Edit this entry! <protect>
| Systematic name | YDL140C |
| Gene name | RPO21 |
| Aliases | RPB1, RPB220, SUA8 |
| Feature type | ORF, Verified |
| Coordinates | Chr IV:210562..205361 |
| Primary SGDID | S000002299 |
Description of YDL140C: RNA polymerase II largest subunit B220, part of central core; phosphorylation of C-terminal heptapeptide repeat domain regulates association with transcription and splicing factors; similar to bacterial beta-prime[1][2][3][4][5][6]
</protect>
Contents
Community Commentary
About Community Commentary. Please share your knowledge!
Rpb1-1 mutation: this is a ts mutant which does not transcribe at the restrictive temperature. According to Scafe, S., Martin, C., Nonet, M., Podos, S., Okamura, S. and Young, R. (MCB 1990 vol. 10(3):1270-1275) this mutation is G->A at base 4622 (base 1 is A of ATG). However, according the Nedea, EC., Markov, D., Naryshkina, T. and Severinov, K. (J. Bact. 1999 Vol 181(8):2663-2665) the rpb1-1 mutation is equivalent to a parallel ts mutation in the E. coli RNA pol, in a conserved Glycine. According to their article, the rpb1-1 mutation is in this conserved Gly (aa 1437, or base 4310) and not Gly 1541 (base 4622 according to R. Young). After sequencing two rpb1-1 mutants in our posession, we agree with Severinov, that the G->A mutation is at base 4310 and NOT in base 4622.
<protect>
References
See Help:References on how to add references
- ↑ Cramer P (2002) Multisubunit RNA polymerases. Curr Opin Struct Biol 12(1):89-97 SGD PMID 11839495
- ↑ Geiduschek EP and Kassavetis GA (2001) The RNA polymerase III transcription apparatus. J Mol Biol 310(1):1-26 SGD PMID 11419933
- ↑ Proudfoot N (2000) Connecting transcription to messenger RNA processing. Trends Biochem Sci 25(6):290-3 SGD PMID 10838569
- ↑ Cramer P, et al. (2000) Architecture of RNA polymerase II and implications for the transcription mechanism. Science 288(5466):640-9 SGD PMID 10784442
- ↑ Hampsey M (1998) Molecular genetics of the RNA polymerase II general transcriptional machinery. Microbiol Mol Biol Rev 62(2):465-503 SGD PMID 9618449
- ↑ Archambault J and Friesen JD (1993) Genetics of eukaryotic RNA polymerases I, II, and III. Microbiol Rev 57(3):703-24 SGD PMID 8246845
See Help:Categories on how to add the wiki page for this gene to a Category </protect>
