Difference between revisions of "YOL069W"

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|valign="top" nowrap bgcolor="{{SGDblue}}"| '''Systematic name''' || [http://db.yeastgenome.org/cgi-bin/locus.pl?locus=YOL069W YOL069W]  
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|valign="top" nowrap bgcolor="{{SGDblue}}"| '''Systematic name''' || [http://www.yeastgenome.org/cgi-bin/locus.pl?dbid=S000005430 YOL069W]  
 
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|valign="top" nowrap bgcolor="{{SGDblue}}"| '''Gene name'''        ||''NUF2 ''
 
|valign="top" nowrap bgcolor="{{SGDblue}}"| '''Gene name'''        ||''NUF2 ''
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|nowrap| Chr XV:198942..200297
 
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|valign="top" nowrap bgcolor="{{SGDblue}}"| '''Primary SGDID'''          || S000005430
 
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'''Description of {{PAGENAME}}:''' Component of the evolutionarily conserved kinetochore-associated Ndc80 complex (Ndc80p-Nuf2p-Spc24p-Spc25p); involved in chromosome segregation, spindle checkpoint activity and kinetochore clustering<ref name='S000080960'>He X, et al. (2001) Molecular analysis of kinetochore-microtubule attachment in budding yeast. Cell 106(2):195-206 {{SGDpaper|S000080960}} PMID 11511347</ref><ref name='S000062011'>Janke C, et al. (2001) The budding yeast proteins Spc24p and Spc25p interact with Ndc80p and Nuf2p at the kinetochore and are important for kinetochore clustering and checkpoint control. EMBO J 20(4):777-91 {{SGDpaper|S000062011}} PMID 11179222</ref><ref name='S000059963'>Wigge PA and Kilmartin JV (2001) The Ndc80p complex from Saccharomyces cerevisiae contains conserved centromere components and has a function in chromosome segregation. J Cell Biol 152(2):349-60 {{SGDpaper|S000059963}} PMID 11266451</ref><ref name='S000041647'>Osborne MA, et al. (1994) Nuf2, a spindle pole body-associated protein required for nuclear division in yeast. J Cell Biol 125(4):853-66
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'''Description of YOL069W:''' Component of the evolutionarily conserved kinetochore-associated Ndc80 complex (Ndc80p-Nuf2p-Spc24p-Spc25p); involved in chromosome segregation, spindle checkpoint activity and kinetochore clustering<ref name='S000080960'>He X, et al. (2001) Molecular analysis of kinetochore-microtubule attachment in budding yeast. Cell 106(2):195-206 {{SGDpaper|S000080960}} PMID 11511347</ref><ref name='S000062011'>Janke C, et al. (2001) The budding yeast proteins Spc24p and Spc25p interact with Ndc80p and Nuf2p at the kinetochore and are important for kinetochore clustering and checkpoint control. EMBO J 20(4):777-91 {{SGDpaper|S000062011}} PMID 11179222</ref><ref name='S000041647'>Osborne MA, et al. (1994) Nuf2, a spindle pole body-associated protein required for nuclear division in yeast. J Cell Biol 125(4):853-66 {{SGDpaper|S000041647}} PMID 8188751</ref><ref name='S000059963'>Wigge PA and Kilmartin JV (2001) The Ndc80p complex from Saccharomyces cerevisiae contains conserved centromere components and has a function in chromosome segregation. J Cell Biol 152(2):349-60
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==Community Commentary==
 
==Community Commentary==
 
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=== Alleles, Strains, and Phenotypes ===
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[[Category:Topic:Alleles, Strains, and Phenotypes]]
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==== Other Alleles, Strains... ====
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[[Category:Topic:Alleles, Strains, and Phenotypes:Other Alleles, Strains...]]
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'''Allele''': nuf2-dt<br>
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'''Other Topic''': degron tagged mutant [[Category:Topic:degron tagged mutant]]
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'''Strain Background''': W303a<br>
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'''Genotype''': 3050 MATa ura3-1 leu2-2,112 his3-11,15 trp1-1 ade2-1 nuf2-dt :URA3 PGAL10-UBR1:HIS3<br>
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'''Phenotype(s)''': Loss of function (Null) [[Category:Phenotype:Loss of function (Null)]], Recessive [[Category:Phenotype:Recessive]], Temperature(Heat) Sensitive [[Category:Phenotype:Temperature(Heat) Sensitive]]
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Degron tagged mutant is spindle checkpoint proficient under restrictive conditions where the protein is eliminated. Mutant arrests in mitosis and the phenotype is similar to nuf2-61. Double mutant with ndc80-1 is checkpoint defective showing that Ndc80 and Nuf2 function redundantly in the spindle checkpoint. <ref name='S000072450'>McCleland ML, et al. (2003) The highly conserved Ndc80 complex is required for kinetochore assembly, chromosome congression, and spindle checkpoint activity. Genes Dev 17(1):101-14 {{SGDpaper|S000072450}} PMID 12514103</ref> <ref name = 'CAset732-2003-02-27'>submitted by [http://db.yeastgenome.org/cgi-bin/colleague/colleagueSearch?id=732 Dan Burke] on 2003-02-27</ref>
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Specifically higher expression in carbon limited chemostat cultures versus carbon excess.
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<ref>Boer VM, et al. (2003) The genome-wide transcriptional responses of Saccharomyces cerevisiae grown on glucose in aerobic chemostat cultures limited for carbon, nitrogen, phosphorus, or sulfur.
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J Biol Chem 278(5):3265-74</ref>
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==References==
 
==References==
 
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Latest revision as of 06:45, 23 January 2012

Share your knowledge...Edit this entry! <protect>

Systematic name YOL069W
Gene name NUF2
Aliases
Feature type ORF, Verified
Coordinates Chr XV:198942..200297
Primary SGDID S000005430


Description of YOL069W: Component of the evolutionarily conserved kinetochore-associated Ndc80 complex (Ndc80p-Nuf2p-Spc24p-Spc25p); involved in chromosome segregation, spindle checkpoint activity and kinetochore clustering[1][2][3][4]




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Community Commentary

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Alleles, Strains, and Phenotypes

Other Alleles, Strains...

Allele: nuf2-dt
Other Topic: degron tagged mutant

Strain Background: W303a
Genotype: 3050 MATa ura3-1 leu2-2,112 his3-11,15 trp1-1 ade2-1 nuf2-dt :URA3 PGAL10-UBR1:HIS3
Phenotype(s): Loss of function (Null), Recessive, Temperature(Heat) Sensitive

Degron tagged mutant is spindle checkpoint proficient under restrictive conditions where the protein is eliminated. Mutant arrests in mitosis and the phenotype is similar to nuf2-61. Double mutant with ndc80-1 is checkpoint defective showing that Ndc80 and Nuf2 function redundantly in the spindle checkpoint. [5] [6]




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References

See Help:References on how to add references

  1. ↑ He X, et al. (2001) Molecular analysis of kinetochore-microtubule attachment in budding yeast. Cell 106(2):195-206 SGD PMID 11511347
  2. ↑ Janke C, et al. (2001) The budding yeast proteins Spc24p and Spc25p interact with Ndc80p and Nuf2p at the kinetochore and are important for kinetochore clustering and checkpoint control. EMBO J 20(4):777-91 SGD PMID 11179222
  3. ↑ Osborne MA, et al. (1994) Nuf2, a spindle pole body-associated protein required for nuclear division in yeast. J Cell Biol 125(4):853-66 SGD PMID 8188751
  4. ↑ Wigge PA and Kilmartin JV (2001) The Ndc80p complex from Saccharomyces cerevisiae contains conserved centromere components and has a function in chromosome segregation. J Cell Biol 152(2):349-60 SGD PMID 11266451
  5. ↑ McCleland ML, et al. (2003) The highly conserved Ndc80 complex is required for kinetochore assembly, chromosome congression, and spindle checkpoint activity. Genes Dev 17(1):101-14 SGD PMID 12514103
  6. ↑ submitted by Dan Burke on 2003-02-27

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