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Systematic name YOL069W
Gene name NUF2
Feature type ORF, Verified
Coordinates Chr XV:198942..200297
Primary SGDID S000005430

Description of YOL069W: Component of the evolutionarily conserved kinetochore-associated Ndc80 complex (Ndc80p-Nuf2p-Spc24p-Spc25p); involved in chromosome segregation, spindle checkpoint activity and kinetochore clustering[1][2][3][4]


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Alleles, Strains, and Phenotypes

Other Alleles, Strains...

Allele: nuf2-dt
Other Topic: degron tagged mutant

Strain Background: W303a
Genotype: 3050 MATa ura3-1 leu2-2,112 his3-11,15 trp1-1 ade2-1 nuf2-dt :URA3 PGAL10-UBR1:HIS3
Phenotype(s): Loss of function (Null), Recessive, Temperature(Heat) Sensitive

Degron tagged mutant is spindle checkpoint proficient under restrictive conditions where the protein is eliminated. Mutant arrests in mitosis and the phenotype is similar to nuf2-61. Double mutant with ndc80-1 is checkpoint defective showing that Ndc80 and Nuf2 function redundantly in the spindle checkpoint. [5] [6]



See Help:References on how to add references

  1. He X, et al. (2001) Molecular analysis of kinetochore-microtubule attachment in budding yeast. Cell 106(2):195-206 SGD PMID 11511347
  2. Janke C, et al. (2001) The budding yeast proteins Spc24p and Spc25p interact with Ndc80p and Nuf2p at the kinetochore and are important for kinetochore clustering and checkpoint control. EMBO J 20(4):777-91 SGD PMID 11179222
  3. Osborne MA, et al. (1994) Nuf2, a spindle pole body-associated protein required for nuclear division in yeast. J Cell Biol 125(4):853-66 SGD PMID 8188751
  4. Wigge PA and Kilmartin JV (2001) The Ndc80p complex from Saccharomyces cerevisiae contains conserved centromere components and has a function in chromosome segregation. J Cell Biol 152(2):349-60 SGD PMID 11266451
  5. McCleland ML, et al. (2003) The highly conserved Ndc80 complex is required for kinetochore assembly, chromosome congression, and spindle checkpoint activity. Genes Dev 17(1):101-14 SGD PMID 12514103
  6. submitted by Dan Burke on 2003-02-27

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