YMR144W

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Systematic name	YMR144W
Gene name
Aliases
Feature type	ORF, Uncharacterized
Coordinates	Chr XIII:553362..554390
Primary SGDID	S000004752

Description of YMR144W: Putative protein of unknown function; localized to the nucleus; YMR144W is not an essential gene




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Isopropanol

UW-Stout/Isopropanol_SP23

Analysis

These results were not consistent as there are 3 very different results. The first trial was way off what it was supposed to be and therefore could have been contaminated or been an error in the pipetting process. The second trial was the most successful and gave the results that were expected. The third trial was good except for the region from about 8:00-11:20 where it spiked up and down. The last 2 had more growth than the control which could mean that the cell without the gene that was knocked out is able to withstand more alcohol.

Glucose Sensitivity

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Analysis

The table above shows the number of cells with YMR144W removed that were counted as dead out of 100 counted cells. We found from our data that there was approximately a 15% decrease in the cell population. It was surprising to find that by removing this gene there was an increase in cell viability. Further research may be required for more conclusive results.

Heat Shock

YMR144W Yeast Colonies at 40 degrees Celsius

Time (sec)	Colonies
0	>100
15	92
45	86
75	100
105	84
135	81

Wild Type Yeast Colonies at 40 degrees Celsius

Time (sec)	Colonies
0	24
15	20
45	17
75	16
105	18
135	14

The general trend of our data yielded a negative relationship between time of heat shock. It can be inferred that with a longer amount of time we the yeast cells could continue being killed off until all of them died.

Bases

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References

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