Difference between revisions of "YJR061W"

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As you can see in the figure above test 4 was thriving and doing well. Tests 1, 2, & 3 were below the growth curve. Growth models 1-3 are The yeast strand YJR061 without any stress.
 
As you can see in the figure above test 4 was thriving and doing well. Tests 1, 2, & 3 were below the growth curve. Growth models 1-3 are The yeast strand YJR061 without any stress.
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===Effect of 3% [[UW Stout/DMSO FA19|DMSO]] on YJR061W===
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[[Image:Effect_of_3%_DMSO_on_YJR061W.png|600px]]
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The graph above shows the effect of 3% DMSO on YJR061W. From the data represented the average doubling rate for the YJR061W knockout strain without DMSO was 591 minutes. When DMSO is added the average doubling time increased to 1331 minutes. This shows that 3% DMSO did add stress to this knockout strain. The average doubling time of WT:BY4735 without DMSO was 514 minutes. The average doubling rate for WT:BY4735 with DMSO was 1529 minutes. If we compare the average doubling rate of WT:BY4735 with DMSO to YJR061W with DMSO we can see that without the YJR061W gene the yeast was able to grow more quickly under DMSO induced stress. It is also observed that both control groups had a very close growth rate, which means that the YJR061W gene has a minimal effect of yeast growth in optimal conditions.
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===[[UW Stout/Proline FA19|Growth in Proline and Ammonium Sulfate]]===
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[[File:KO9.png|650 px]]
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For the YJR061W strand, it grew faster in the proline media with a average doubling time of 36.53 minutes. It grew with an average doubling time of 45.67 minutes in ammonium sulfate media. The wild types were peculiar in these tests because they had the exact same average doubling time of 44.73 minutes.
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===1% Formamide Yeast Torture[[https://wiki.yeastgenome.org/index.php/UW_Stout/Formamide_FA19]]===
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[[Image:ko9.png]]
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This graph shows the growth curve of the knockout yeast strain YJR061W and wild type BY4735 in 1% formamide in 3 trials. The average calculated doubling time for YJR061W with 1% formamide was 2,098.56 minutes, while the unstressed strain was 2,966.34 minutes. This concluded that the stressed out strain is not sensitive to 1% formamide, and grows at a faster pace in this environment. Our stressed wild types doubling time was 2,865.52 minutes, and the unstressed was 2,468.04 minutes. Proving that the addition of 1% formamide makes the stressed strain more sensitive to it's surroundings and leads to slower growth.
  
 
==Ethanol Tolerance in YJR061W==
 
==Ethanol Tolerance in YJR061W==
 
[[Image:YJR061W_ethonal.png]]
 
[[Image:YJR061W_ethonal.png]]
  
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This graph shows the growth between YJR061W treated and untreated with ethanol. The yeast treated with ethanol had a growth rate of (answer). The yeast not treated with ethanol had a growth rate of (answer). This shows that the strands of YJR061W treated with ethanol had an increased growth rate compared to those who weren't.  
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This graph shows the growth between YJR061W treated and untreated with ethanol. The yeast treated with ethanol had a growth rate of 1418.74 minutes. The yeast not treated with ethanol had a growth rate of 1822.83. This shows that the strands of YJR061W treated with ethanol had a decrease growth rate compared to those who weren't.  
  
 
===[[UW-Stout/Prednisone_FA19|Prednisone Effects on YJR061W]]===
 
===[[UW-Stout/Prednisone_FA19|Prednisone Effects on YJR061W]]===
 
[[File:YJR061W.png|750 px]]
 
[[File:YJR061W.png|750 px]]
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The graph above shows how dilutions P2 (30 µg/ml) and E2 (10% ethanol solution) impacted the growth rate of the knockout yeast strain YJR061W over 24 hours. The concentration did not double for test 3 of P2 for this experiment, so by taking only the first two test results, the doubling rate averages to around 907.5 minutes for only ethanol. As for the Prednisone with ethanol, the doubling rate averages to around 907.5 minutes. The Prednisone with ethanol typically has the same growth rate as the ethanol alone for this strain.
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[[File:Nitrogen_Starvation_YJR061W.jpg|463 x 599|Caption]]
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The Wild Type BY4735 has data located at the Nitrogen Starvation Protocol.
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==[[UW-Stout/Nitrogen_starvation_FA19|Nitrogen starvation]]==
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== [https://wiki.yeastgenome.org/index.php?title=UW-Stout/Hydroxyurea_FA19&oldid=401696 Hydroxyurea] ==
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[[File:AvaYJR061W.png]]
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The averaging doubling time for YJR061W is 181.99 minutes. The averaging doubling time for the unstressed YJR061W is 437.11 minutes
  
 
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===Zone of Inhibition of 30% [https://wiki.yeastgenome.org/index.php/UW-Stout/Hydrogen_peroxide_FA19 Hydrogen Peroxide] on YJR061W===
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[[File:JJ9.PNG]]
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[[File:JaredJ9.PNG]]
  
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The bar graph represents the zone of inhibition of the knockout strain versus the wild type. Our data collected shows that the knockout strain saw a larger zone of inhibition (cells were not able to grow there). Using these observations it is evident that the yeast strain YJR061W undergoes more stress from the oxidative properties of 30 percent hydrogen peroxide. The zone of inhibition is larger because the yeast cells are not able to grow as well as the wild type can in these conditions. For the strain YJR061W our plate values were as follows (in cm) 2.4, 2.4 compared to our wild type plate which stayed constant 2.1, 2.1 and 2.2 cm
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*Note our third plate saw negative results as the disc which contained hydrogen peroxide adjusted its placing and we could not get a good reading for the zone of inhibition which is why we did this experiment three times. Two averages will make us deviate further from the mean.
 
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Latest revision as of 09:37, 18 December 2019

Share your knowledge...Edit this entry! <protect>

Systematic name YJR061W
Gene name
Aliases
Feature type ORF, Uncharacterized
Coordinates Chr X:550511..553318
Primary SGDID S000003822


Description of YJR061W: Putative protein of unknown function; non-essential gene with similarity to Mnn4, a putative membrane protein involved in glycosylation; transcription repressed by Rm101p[1][2]




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Community Commentary

About Community Commentary. Please share your knowledge!

This gene is part of the UW-Stout Orphan Gene Project. Learn more here.

Growth in YPD

YJR061W YPD.png

In a BY4735 background, knocking out YJR061W seems to have little to no effect on growth rate in log-phase. In this assay, the BY4735 strain's doubling time was 414 minutes, while the YJR061W knock-out strain's doubling time was 436 minutes. (These doubling times are the means of three experiments.)

Caffeine and Yeast Cells

megan28.png

This graph shows the growth curve with and without caffeine. It has the knockout strain and wild type yeast cells. The caffeine enhanced the growth in both wild type and knockout strain. The average doubling time between the three experiments for the knockout strand was 824.60 minutes. The caffeine also had a positive effect on the growth curve for the wild type with the average doubling time being 1245.02 minutes. The results were concluded from the wild type and knockout strand was with this amount of caffeine it leads to an increase in growth.

  • Note: The graph should be labeled YJK061W

The protocol can be found at

Caffeine

yJR061wUV Exposure.png

This bar graph showsthat compared to a BY4735 background, Knocking out yJR061w makes yeast a little bit more sensitive to UV light.

The protocol can be found at

UV Light Exposure

Cycloheximide effecting YJR061W

Cycloheximide 10.png

As you can see in the figure above test 4 was thriving and doing well. Tests 1, 2, & 3 were below the growth curve. Growth models 1-3 are The yeast strand YJR061 without any stress.


Effect of 3% DMSO on YJR061W

Effect of 3% DMSO on YJR061W.png

The graph above shows the effect of 3% DMSO on YJR061W. From the data represented the average doubling rate for the YJR061W knockout strain without DMSO was 591 minutes. When DMSO is added the average doubling time increased to 1331 minutes. This shows that 3% DMSO did add stress to this knockout strain. The average doubling time of WT:BY4735 without DMSO was 514 minutes. The average doubling rate for WT:BY4735 with DMSO was 1529 minutes. If we compare the average doubling rate of WT:BY4735 with DMSO to YJR061W with DMSO we can see that without the YJR061W gene the yeast was able to grow more quickly under DMSO induced stress. It is also observed that both control groups had a very close growth rate, which means that the YJR061W gene has a minimal effect of yeast growth in optimal conditions.


Growth in Proline and Ammonium Sulfate

KO9.png


For the YJR061W strand, it grew faster in the proline media with a average doubling time of 36.53 minutes. It grew with an average doubling time of 45.67 minutes in ammonium sulfate media. The wild types were peculiar in these tests because they had the exact same average doubling time of 44.73 minutes.

1% Formamide Yeast Torture[[1]]

ko9.png

This graph shows the growth curve of the knockout yeast strain YJR061W and wild type BY4735 in 1% formamide in 3 trials. The average calculated doubling time for YJR061W with 1% formamide was 2,098.56 minutes, while the unstressed strain was 2,966.34 minutes. This concluded that the stressed out strain is not sensitive to 1% formamide, and grows at a faster pace in this environment. Our stressed wild types doubling time was 2,865.52 minutes, and the unstressed was 2,468.04 minutes. Proving that the addition of 1% formamide makes the stressed strain more sensitive to it's surroundings and leads to slower growth.

Ethanol Tolerance in YJR061W

YJR061W ethonal.png

This graph shows the growth between YJR061W treated and untreated with ethanol. The yeast treated with ethanol had a growth rate of 1418.74 minutes. The yeast not treated with ethanol had a growth rate of 1822.83. This shows that the strands of YJR061W treated with ethanol had a decrease growth rate compared to those who weren't.

Prednisone Effects on YJR061W

YJR061W.png

The graph above shows how dilutions P2 (30 µg/ml) and E2 (10% ethanol solution) impacted the growth rate of the knockout yeast strain YJR061W over 24 hours. The concentration did not double for test 3 of P2 for this experiment, so by taking only the first two test results, the doubling rate averages to around 907.5 minutes for only ethanol. As for the Prednisone with ethanol, the doubling rate averages to around 907.5 minutes. The Prednisone with ethanol typically has the same growth rate as the ethanol alone for this strain.

Caption

The Wild Type BY4735 has data located at the Nitrogen Starvation Protocol.

Nitrogen starvation

Hydroxyurea

AvaYJR061W.png The averaging doubling time for YJR061W is 181.99 minutes. The averaging doubling time for the unstressed YJR061W is 437.11 minutes


Zone of Inhibition of 30% Hydrogen Peroxide on YJR061W

JJ9.PNG JaredJ9.PNG

The bar graph represents the zone of inhibition of the knockout strain versus the wild type. Our data collected shows that the knockout strain saw a larger zone of inhibition (cells were not able to grow there). Using these observations it is evident that the yeast strain YJR061W undergoes more stress from the oxidative properties of 30 percent hydrogen peroxide. The zone of inhibition is larger because the yeast cells are not able to grow as well as the wild type can in these conditions. For the strain YJR061W our plate values were as follows (in cm) 2.4, 2.4 compared to our wild type plate which stayed constant 2.1, 2.1 and 2.2 cm

  • Note our third plate saw negative results as the disc which contained hydrogen peroxide adjusted its placing and we could not get a good reading for the zone of inhibition which is why we did this experiment three times. Two averages will make us deviate further from the mean.

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References

See Help:References on how to add references

  1. ↑ Conde R, et al. (2003) Screening for new yeast mutants affected in mannosylphosphorylation of cell wall mannoproteins. Yeast 20(14):1189-211 SGD PMID 14587103
  2. ↑ Lamb TM and Mitchell AP (2003) The transcription factor Rim101p governs ion tolerance and cell differentiation by direct repression of the regulatory genes NRG1 and SMP1 in Saccharomyces cerevisiae. Mol Cell Biol 23(2):677-86 SGD PMID 12509465

See Help:Categories on how to add the wiki page for this gene to a Category </protect>

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