Difference between revisions of "YGL140C"
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The protocol can be found at | The protocol can be found at | ||
===[[UW_Stout/UV_Light_FA19|UV Light Exposure]]=== | ===[[UW_Stout/UV_Light_FA19|UV Light Exposure]]=== | ||
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| + | ===Cycloheximide effecting YGL140C=== | ||
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| + | [[File:Cycloheximide 9.png]] | ||
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| + | As you can see from the picture above test 4 did really well and survived while tests 1-3 were disabled and didn't function as well. Growth models 1-3 are the factors that were not stressed. | ||
==Ethanol Tolerance in YGL140C== | ==Ethanol Tolerance in YGL140C== | ||
Revision as of 14:46, 12 December 2019
Share your knowledge...Edit this entry! <protect>
| Systematic name | YGL140C |
| Gene name | |
| Aliases | |
| Feature type | ORF, Uncharacterized |
| Coordinates | Chr VII:245012..241353 |
| Primary SGDID | S000003108 |
Description of YGL140C: Putative protein of unknown function; non-essential gene; contains multiple predicted transmembrane domains[1][2][3]
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Contents
Community Commentary
About Community Commentary. Please share your knowledge!
This gene is part of the UW-Stout Orphan Gene Project. Learn more here.
Growth Curve
In a BY4735 background, knocking out YGL140C seems to have little or no effect on growth rate in log-phase. In this assay, the BY4735 strain's doubling time was 414 minutes, while the YGL140C knock-out strain's doubling time was 432 minutes. (These doubling times are the means of three experiments.)
Caffeine and Yeast Cells
This graph shows the growth curve with and without caffeine. It has the knockout strain and wild type yeast cells. The caffeine enhanced the growth in both wild type and knockout strain. The average doubling time between the three experiments for the knockout strand was 777.97 minutes. The caffeine also had a positive effect on the growth curve for the wild type with the average doubling time being 1245.02 minutes. The results were concluded from the wild type and knockout strand was with this amount of caffeine it leads to an increase in growth.
The protocol can be found at
Caffeine
This bar graph shows that compared to a BY4735 background' Knocking out yGL140c have no effect on how sensitive yeast is to UV Light
The protocol can be found at
UV Light Exposure
Cycloheximide effecting YGL140C
As you can see from the picture above test 4 did really well and survived while tests 1-3 were disabled and didn't function as well. Growth models 1-3 are the factors that were not stressed.
Ethanol Tolerance in YGL140C
This graph shows the rate of growth between YGL140C treated and untreated with ethanol. The rate of growth of the yeast treated with ethanol is (answer). The rate of growth of the yeast untreated with ethanol is (answer). This shows that the strands of YGL140C treated with ethanol had an increased rate of growth compared to those who weren't.
Prednisone Effects on YGL140C
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References
See Help:References on how to add references
- ↑ Giaever G, et al. (2002) Functional profiling of the Saccharomyces cerevisiae genome. Nature 418(6896):387-91 SGD PMID 12140549
- ↑ Voet M, et al. (1997) The sequence of a nearly unclonable 22.8 kb segment on the left arm chromosome VII from Saccharomyces cerevisiae reveals ARO2, RPL9A, TIP1, MRF1 genes and six new open reading frames. Yeast 13(2):177-82 SGD PMID 9046099
- ↑ Volckaert G, et al. (2003) Disruption of 12 ORFs located on chromosomes IV, VII and XIV of Saccharomyces cerevisiae reveals two essential genes. Yeast 20(1):79-88 SGD PMID 12489128
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