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− | === Alleles, Strains, and Phenotypes ===
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− | [[Category:Topic:Alleles, Strains, and Phenotypes]]
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− | ==== Multiple Knockout Strains ====
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− | [[Category:Topic:Alleles, Strains, and Phenotypes:Multiple Knockout Strains]]
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− | '''Strain Background''': Sigma1278b (Sigma 2000 series)<br>
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− | '''Genotype''': MCY4708<br>
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− | '''Together with''': NRG1, SIP1, GAL83, SIP2<br>
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− | '''Phenotype(s)''': Loss of function (Null) [[Category:Phenotype:Loss of function (Null)]]
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− | Deletion of nrg2 partially suppresses gal83's defect in invasive growth, plastic adherance, and STA2-lacZ expression. <ref name='S000072732'>Vyas VK, et al. (2003) Snf1 kinases with different beta-subunit isoforms play distinct roles in regulating haploid invasive growth. Mol Cell Biol 23(4):1341-8 {{SGDpaper|S000072732}} PMID 12556493</ref> <ref name = 'CAset6176-2003-03-27'>submitted by [http://db.yeastgenome.org/cgi-bin/colleague/colleagueSearch?id=6176 Valmik K. Vyas] on 2003-03-27</ref>
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− | {{ShortCenteredHR}}
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− | '''Strain Background''': Sigma1278b (Sigma 2000 series)<br>
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− | '''Genotype''': MCY4712<br>
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− | '''Together with''': NRG1, SIP1, GAL83, SIP2<br>
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− | '''Phenotype(s)''': Loss of function (Null) [[Category:Phenotype:Loss of function (Null)]]
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− |
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− | Deletion of nrg1 and nrg2 suppresses gal83's defect in invasive growth, plastic adherance, and STA2-lacZ expression, better than deletion of either nrg alone. <ref name='S000072732'>Vyas VK, et al. (2003) Snf1 kinases with different beta-subunit isoforms play distinct roles in regulating haploid invasive growth. Mol Cell Biol 23(4):1341-8 {{SGDpaper|S000072732}} PMID 12556493</ref> <ref name = 'CAset6176-2003-03-27'>submitted by [http://db.yeastgenome.org/cgi-bin/colleague/colleagueSearch?id=6176 Valmik K. Vyas] on 2003-03-27</ref>
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− |
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− | {{ShortCenteredHR}}
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− |
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− | '''Strain Background''': Sigma1278b (Sigma 2000 series)<br>
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− | '''Genotype''': MCY4708<br>
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− | '''Together with''': NRG1, SIP1, GAL83, SIP2<br>
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− | '''Phenotype(s)''': Loss of function (Null) [[Category:Phenotype:Loss of function (Null)]]
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− |
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− | Deletion of nrg2 partially suppresses gal83's defect in invasive growth, plastic adherance, and STA2-lacZ expression. <ref name='S000072732'>Vyas VK, et al. (2003) Snf1 kinases with different beta-subunit isoforms play distinct roles in regulating haploid invasive growth. Mol Cell Biol 23(4):1341-8 {{SGDpaper|S000072732}} PMID 12556493</ref> <ref name = 'CAset6176-2003-03-27'>submitted by [http://db.yeastgenome.org/cgi-bin/colleague/colleagueSearch?id=6176 Valmik K. Vyas] on 2003-03-27</ref>
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− |
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− | {{ShortCenteredHR}}
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− |
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− | '''Strain Background''': Sigma1278b (Sigma 2000 series)<br>
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− | '''Genotype''': MCY4712<br>
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− | '''Together with''': NRG1, SIP1, GAL83, SIP2<br>
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− | '''Phenotype(s)''': Loss of function (Null) [[Category:Phenotype:Loss of function (Null)]]
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− |
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− | Deletion of nrg1 and nrg2 suppresses gal83's defect in invasive growth, plastic adherance, and STA2-lacZ expression, better than deletion of either nrg alone. <ref name='S000072732'>Vyas VK, et al. (2003) Snf1 kinases with different beta-subunit isoforms play distinct roles in regulating haploid invasive growth. Mol Cell Biol 23(4):1341-8 {{SGDpaper|S000072732}} PMID 12556493</ref> <ref name = 'CAset6176-2003-03-27'>submitted by [http://db.yeastgenome.org/cgi-bin/colleague/colleagueSearch?id=6176 Valmik K. Vyas] on 2003-03-27</ref>
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− | {{ShortCenteredHR}}
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− | ==== Overexpression ====
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− | [[Category:Topic:Alleles, Strains, and Phenotypes:Overexpression]]
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− | '''Phenotype(s)''': Dominant [[Category:Phenotype:Dominant]], Gain of function [[Category:Phenotype:Gain of function]]
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− | Overexpression of VP16-Nrg2 results in partial derepression of SUC2 in glucose grown cells. <ref name='S000060795'>Vyas VK, et al. (2001) Interaction of the repressors Nrg1 and Nrg2 with the Snf1 protein kinase in Saccharomyces cerevisiae. Genetics 158(2):563-72 {{SGDpaper|S000060795}} PMID 11404322</ref> <ref name = 'CAset6176-2003-02-04'>submitted by [http://db.yeastgenome.org/cgi-bin/colleague/colleagueSearch?id=6176 Valmik K. Vyas] on 2003-02-04</ref>
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− | {{ShortCenteredHR}}
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− | === Interactions ===
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− | [[Category:Topic:Interactions]]
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− | ==== Genetic ====
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− | [[Category:Topic:Interactions:Genetic]]
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− | YBR066C is a suppressor of Snf1 [[Category:Genetic Interaction with Snf1]]
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− | '''Strain Background''': Sigma 1278b<br>
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− | '''Mutation type(s)''': complete deletion, complete deletion
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− | Deletion of Nrg2 partially suppresses Snf1's defect in invasive growth, biofilm formation, FLO11 expression, and STA2 expression. <ref name='S000070028'>Kuchin S, et al. (2002) Snf1 protein kinase and the repressors Nrg1 and Nrg2 regulate FLO11, haploid invasive growth, and diploid pseudohyphal differentiation. Mol Cell Biol 22(12):3994-4000 {{SGDpaper|S000070028}} PMID 12024013</ref> <ref name = 'CAset6176-2003-02-04'>submitted by [http://db.yeastgenome.org/cgi-bin/colleague/colleagueSearch?id=6176 Valmik K. Vyas] on 2003-02-04</ref>
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− |
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− | {{ShortCenteredHR}}
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− | ==== Physical ====
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− | [[Category:Topic:Interactions:Physical]]
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− | Physical interaction with Snf1 [[Category:Physical Interactions with Snf1]]<br>
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− | LexA-Snf1 coimmunoprecipitates HA3-Nrg2 <ref name='S000060795'>Vyas VK, et al. (2001) Interaction of the repressors Nrg1 and Nrg2 with the Snf1 protein kinase in Saccharomyces cerevisiae. Genetics 158(2):563-72 {{SGDpaper|S000060795}} PMID 11404322</ref> <ref name = 'CAset6176-2003-02-04'>submitted by [http://db.yeastgenome.org/cgi-bin/colleague/colleagueSearch?id=6176 Valmik K. Vyas] on 2003-02-04</ref>
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− | {{ShortCenteredHR}}
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− | Physical interaction with Snf1 [[Category:Physical Interactions with Snf1]]<br>
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− | Snf1 coimmunoprecipitates with HA3-Nrg2 <ref name='S000060795'>Vyas VK, et al. (2001) Interaction of the repressors Nrg1 and Nrg2 with the Snf1 protein kinase in Saccharomyces cerevisiae. Genetics 158(2):563-72 {{SGDpaper|S000060795}} PMID 11404322</ref> <ref name = 'CAset6176-2003-02-04'>submitted by [http://db.yeastgenome.org/cgi-bin/colleague/colleagueSearch?id=6176 Valmik K. Vyas] on 2003-02-04</ref>
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− | {{ShortCenteredHR}}
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− | ==== Two Hybrid ====
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− | [[Category:Topic:Interactions:Two Hybrid]]
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− | Two Hybrid interaction with Snf1 [[Category:Two Hybrid Interactions with Snf1]]<br>
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− | Snf1 kinase domain interacts with Nrg2 <ref name='S000060795'>Vyas VK, et al. (2001) Interaction of the repressors Nrg1 and Nrg2 with the Snf1 protein kinase in Saccharomyces cerevisiae. Genetics 158(2):563-72 {{SGDpaper|S000060795}} PMID 11404322</ref> <ref name = 'CAset6176-2003-02-04'>submitted by [http://db.yeastgenome.org/cgi-bin/colleague/colleagueSearch?id=6176 Valmik K. Vyas] on 2003-02-04</ref>
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− | {{ShortCenteredHR}}
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− | === Protein Details ===
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− | [[Category:Topic:Protein Details]]
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− | ==== Protein Function/Process ====
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− | [[Category:Topic:Protein Details:Protein Function/Process]]
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− |
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− | LexA-Nrg2 represses transcription from a heterologous promoter containing LexA binding sites. <ref name='S000060795'>Vyas VK, et al. (2001) Interaction of the repressors Nrg1 and Nrg2 with the Snf1 protein kinase in Saccharomyces cerevisiae. Genetics 158(2):563-72 {{SGDpaper|S000060795}} PMID 11404322</ref> <ref name = 'CAset6176-2003-02-04'>submitted by [http://db.yeastgenome.org/cgi-bin/colleague/colleagueSearch?id=6176 Valmik K. Vyas] on 2003-02-04</ref>
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− | {{ShortCenteredHR}}
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| __TOC__ | | __TOC__ |
| ==Community Commentary== | | ==Community Commentary== |
Share your knowledge...Edit this entry!
Systematic name |
YBR066C
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Gene name |
NRG2
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Aliases |
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Feature type |
ORF, Verified
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Coordinates
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Chr II:370697..370035
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Don't edit this box! It's automatically regenerated, and edits will be lost when the update script runs.
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Description of YBR066C: Transcriptional repressor that mediates glucose repression and negatively regulates filamentous growth; has similarity to Nrg1p[1][2]
About Community Commentary. Please share your knowledge!
Alleles, Strains, and Phenotypes
Multiple Knockout Strains
Strain Background: Sigma1278b (Sigma 2000 series)
Genotype: MCY4708
Together with: NRG1, SIP1, GAL83, SIP2
Phenotype(s): Loss of function (Null)
Deletion of nrg2 partially suppresses gal83's defect in invasive growth, plastic adherance, and STA2-lacZ expression. [3] [4]
Strain Background: Sigma1278b (Sigma 2000 series)
Genotype: MCY4712
Together with: NRG1, SIP1, GAL83, SIP2
Phenotype(s): Loss of function (Null)
Deletion of nrg1 and nrg2 suppresses gal83's defect in invasive growth, plastic adherance, and STA2-lacZ expression, better than deletion of either nrg alone. [3] [4]
Strain Background: Sigma1278b (Sigma 2000 series)
Genotype: MCY4708
Together with: NRG1, SIP1, GAL83, SIP2
Phenotype(s): Loss of function (Null)
Deletion of nrg2 partially suppresses gal83's defect in invasive growth, plastic adherance, and STA2-lacZ expression. [3] [4]
Strain Background: Sigma1278b (Sigma 2000 series)
Genotype: MCY4712
Together with: NRG1, SIP1, GAL83, SIP2
Phenotype(s): Loss of function (Null)
Deletion of nrg1 and nrg2 suppresses gal83's defect in invasive growth, plastic adherance, and STA2-lacZ expression, better than deletion of either nrg alone. [3] [4]
Overexpression
Phenotype(s): Dominant, Gain of function
Overexpression of VP16-Nrg2 results in partial derepression of SUC2 in glucose grown cells. [2] [5]
Interactions
Genetic
YBR066C is a suppressor of Snf1
Strain Background: Sigma 1278b
Mutation type(s): complete deletion, complete deletion
Deletion of Nrg2 partially suppresses Snf1's defect in invasive growth, biofilm formation, FLO11 expression, and STA2 expression. [1] [5]
Physical
Physical interaction with Snf1
LexA-Snf1 coimmunoprecipitates HA3-Nrg2 [2] [5]
Physical interaction with Snf1
Snf1 coimmunoprecipitates with HA3-Nrg2 [2] [5]
Two Hybrid
Two Hybrid interaction with Snf1
Snf1 kinase domain interacts with Nrg2 [2] [5]
Protein Details
Protein Function/Process
LexA-Nrg2 represses transcription from a heterologous promoter containing LexA binding sites. [2] [5]
References
See Help:References on how to add references
- ↑ 1.0 1.1 Kuchin S, et al. (2002) Snf1 protein kinase and the repressors Nrg1 and Nrg2 regulate FLO11, haploid invasive growth, and diploid pseudohyphal differentiation. Mol Cell Biol 22(12):3994-4000 SGD PMID 12024013
- ↑ 2.0 2.1 2.2 2.3 2.4 2.5 Vyas VK, et al. (2001) Interaction of the repressors Nrg1 and Nrg2 with the Snf1 protein kinase in Saccharomyces cerevisiae. Genetics 158(2):563-72
SGD PMID 11404322 Cite error: Invalid
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- ↑ 3.0 3.1 3.2 3.3 Vyas VK, et al. (2003) Snf1 kinases with different beta-subunit isoforms play distinct roles in regulating haploid invasive growth. Mol Cell Biol 23(4):1341-8 SGD PMID 12556493
- ↑ 4.0 4.1 4.2 4.3 submitted by Valmik K. Vyas on 2003-03-27
- ↑ 5.0 5.1 5.2 5.3 5.4 5.5 submitted by Valmik K. Vyas on 2003-02-04
See Help:Categories on how to add the wiki page for this gene to a Category