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Systematic name YKL089W
Gene name MIF2
Feature type ORF, Verified
Coordinates Chr XI:273394..275043
Primary SGDID S000001572

Description of YKL089W: Protein required for structural integrity of elongating spindles; localizes to the kinetochore; interacts with histones H2A, H2B, and H4; phosphorylated by Ipl1p; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-C and fission yeast cnp3[1][2][3][4]


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Strain Background: A364A/S288C
Genetic interaction with CBF2
Phenotype(s): Synthetic Lethal with CBF2 Mutation type(s): temperature sensitive missense

at the time showed that MIF2 genetically interacts with an "established" centromere protein gene [2] [5]


Physical interaction with CSE4
Cse4p immunoprecipitates with Mif2p. [6] [7]

Physical interaction with DSN1
Mif2p co-immunoprecipitates Dsn1p. [6] [7]

Protein Details

Other Protein Details

Other Topic: Homology

MIF2 contains two regions of high sequence similarity to human CENP-C. [2] [5]



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  1. Brown MT, et al. (1993) MIF2 is required for mitotic spindle integrity during anaphase spindle elongation in Saccharomyces cerevisiae. J Cell Biol 123(2):387-403 SGD PMID 8408221
  2. 2.0 2.1 2.2 Meluh PB and Koshland D (1995) Evidence that the MIF2 gene of Saccharomyces cerevisiae encodes a centromere protein with homology to the mammalian centromere protein CENP-C. Mol Biol Cell 6(7):793-807 SGD PMID 7579695
  3. Schleiffer A, et al. (2012) CENP-T proteins are conserved centromere receptors of the Ndc80 complex. Nat Cell Biol 14(6):604-13 SGD PMID 22561346
  4. Westermann S, et al. (2003) Architecture of the budding yeast kinetochore reveals a conserved molecular core. J Cell Biol 163(2):215-22 SGD PMID 14581449
  5. 5.0 5.1 submitted by Pamela B. Meluh on 2003-03-17
  6. 6.0 6.1 Pinsky BA, et al. (2003) An Mtw1 complex promotes kinetochore biorientation that is monitored by the Ipl1/Aurora protein kinase. Dev Cell 5(5):735-45 SGD PMID 14602074
  7. 7.0 7.1 submitted by Benjamin Pinsky on 2004-03-04

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