YER027C
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| Systematic name | YER027C |
| Gene name | GAL83 |
| Aliases | SPM1 |
| Feature type | ORF, Verified |
| Coordinates | Chr V:210232..208979 |
| Primary SGDID | S000000829 |
Description of YER027C: One of three possible beta-subunits of the Snf1 kinase complex, allows nuclear localization of the Snf1 kinase complex in the presence of a nonfermentable carbon source; contains glycogen-binding domain[1][2]
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Contents
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Alleles, Strains, and Phenotypes
Complete Deletion
Allele: gal83delta
Strain Background: Sigma1278b (Sigma2000 series)
Genotype: MCY4567
Together with: NRG2, NRG1, SIP1, SIP2
Phenotype(s): Loss of function (Null)
gal83 mutants are defective in adherance to plastic in response to limiting glucose. [3] [4]
Allele: gal83delta
Strain Background: Sigma1278b
Genotype: MCY4567
Together with: NRG2, NRG1, SIP1, SIP2
Phenotype(s): Loss of function (Null)
gal83 mutants are defective in invasive growth in response to limiting glucose. [3] [4]
Allele: gal83delta
Strain Background: Sigma1278b (Sigma2000 series)
Genotype: MCY4567
Together with: NRG2, NRG1, SIP1, SIP2
Phenotype(s): Loss of function (Null)
gal83 mutants are defective in adherance to plastic in response to limiting glucose. [3] [4]
Allele: gal83 delta
Strain Background: Sigma1278b (Sigma2000 series)
Genotype: MCY 4567
Together with: NRG2, NRG1, SIP1, SIP2
Phenotype(s): Loss of function (Null)
gal83 mutants exhibit wild-type filamentation in response to glucose depletion. [3] [4]
Allele: gal83delta
Strain Background: Sigma1278b
Genotype: MCY4567
Together with: NRG2, NRG1, SIP1, SIP2
Phenotype(s): Loss of function (Null)
gal83 mutants are defective in invasive growth in response to limiting glucose. [3] [4]
Allele: gal83 delta
Strain Background: Sigma1278b (Sigma2000 series)
Genotype: MCY 4567
Together with: NRG2, NRG1, SIP1, SIP2
Phenotype(s): Loss of function (Null)
gal83 mutants exhibit wild-type filamentation in response to glucose depletion. [3] [4]
Multiple Knockout Strains
Strain Background: Sigma1278b (Sigma 2000 series)
Genotype: MCY4708
Together with: NRG2, NRG1, SIP1, SIP2
Phenotype(s): Loss of function (Null)
Deletion of nrg2 partially suppresses gal83's defect in invasive growth, plastic adherance, and STA2-lacZ expression. [3] [4]
Strain Background: Sigma1278b (Sigma 2000 series)
Genotype: MCY4716
Together with: NRG2, NRG1, SIP1, SIP2
Phenotype(s): Loss of function (Null)
Deletion of nrg1 partially suppresses gal83's defect in invasive growth, plastic adherance, and STA2-lacZ expression. [3] [4]
Strain Background: Sigma1278b (Sigma2000 series)
Genotype: MCY 4597
Together with: NRG2, NRG1, SIP1, SIP2
Phenotype(s): Loss of function (Null)
sip2 gal83 double mutants are more defective for invasive growth in response to limiting glucose than gal83 single mutants. [3] [4]
Strain Background: Sigma1278b (Sigma2000 series)
Genotype: MCY4581
Together with: NRG2, NRG1, SIP1, SIP2
Phenotype(s): Loss of function (Null)
Deletion of SIP1 suppresses gal83's defect in invasive growth in response to glucose limitation. [3] [4]
Strain Background: Sigma1278b (Sigma 2000 series)
Genotype: MCY4712
Together with: NRG2, NRG1, SIP1, SIP2
Phenotype(s): Loss of function (Null)
Deletion of nrg1 and nrg2 suppresses gal83's defect in invasive growth, plastic adherance, and STA2-lacZ expression, better than deletion of either nrg alone. [3] [4]
Strain Background: Sigma1278b (Sigma 2000 series)
Genotype: MCY4597
Together with: NRG2, NRG1, SIP1, SIP2
Phenotype(s): Loss of function (Null)
sip2 gal83 double mutants are defective for filamentation in response to glucose depletion. [3] [4]
Strain Background: Sigma1278b (Sigma2000 series)
Genotype: MCY4581
Together with: NRG2, NRG1, SIP1, SIP2
Phenotype(s): Loss of function (Null)
Deletion of SIP1 suppresses gal83's defect in invasive growth in response to glucose limitation. [3] [4]
Strain Background: Sigma1278b (Sigma2000 series)
Genotype: MCY 4597
Together with: NRG2, NRG1, SIP1, SIP2
Phenotype(s): Loss of function (Null)
sip2 gal83 double mutants are more defective for invasive growth in response to limiting glucose than gal83 single mutants. [3] [4]
Strain Background: Sigma1278b (Sigma 2000 series)
Genotype: MCY4716
Together with: NRG2, NRG1, SIP1, SIP2
Phenotype(s): Loss of function (Null)
Deletion of nrg1 partially suppresses gal83's defect in invasive growth, plastic adherance, and STA2-lacZ expression. [3] [4]
Strain Background: Sigma1278b (Sigma 2000 series)
Genotype: MCY4708
Together with: NRG2, NRG1, SIP1, SIP2
Phenotype(s): Loss of function (Null)
Deletion of nrg2 partially suppresses gal83's defect in invasive growth, plastic adherance, and STA2-lacZ expression. [3] [4]
Strain Background: Sigma1278b (Sigma2000 series)
Genotype: MCY4600
Together with: REG1, SIP1, SIP2, MUC1
Phenotype(s): Loss of function (Null)
Deletion of SIP1 in a gal83 sip2 double mutant causes an increase in invasion, suggesting Sip1 negatively regulates invasive growth. [3] [4]
Strain Background: Sigma1278b (Sigma2000 series)
Genotype: MCY4571
Together with: REG1, SIP1, SIP2, MUC1
Phenotype(s): Loss of function (Null)
Deletion of GAL83 suppresses reg1's constitutive adherance to plastic (adherance in high glucose (2%)). [3] [4]
Strain Background: Sigma1278b (Sigma 2000 series)
Genotype: MCY4633
Together with: REG1, SIP1, SIP2, MUC1
Phenotype(s): Loss of function (Null)
Deletion of both SIP2 and GAL83 in a reg1 mutant suppresses its constitutive filamentous phenotype. [3] [4]
Strain Background: Sigma1278b (Sigma2000 series)
Genotype: MCY4600
Together with: REG1, SIP1, SIP2, MUC1
Phenotype(s): Loss of function (Null)
Deletion of SIP1 in a gal83 sip2 double mutant causes an increase in invasion, suggesting Sip1 negatively regulates invasive growth. [3] [4]
Strain Background: Sigma1278b (Sigma2000 series)
Genotype: MCY4571
Together with: REG1, SIP1, SIP2, MUC1
Phenotype(s): Loss of function (Null)
Deletion of GAL83 suppresses reg1's constitutive adherance to plastic (adherance in high glucose (2%)). [3] [4]
Strain Background: Sigma1278b (Sigma 2000 series)
Genotype: MCY4633
Together with: REG1, SIP1, SIP2, MUC1
Phenotype(s): Loss of function (Null)
Deletion of both SIP2 and GAL83 in a reg1 mutant suppresses its constitutive filamentous phenotype. [3] [4]
Strain Background: Sigma1278b (Sigma 2000 series)
Genotype: MCY4712
Together with: NRG2, NRG1, SIP1, SIP2
Phenotype(s): Loss of function (Null)
Deletion of nrg1 and nrg2 suppresses gal83's defect in invasive growth, plastic adherance, and STA2-lacZ expression, better than deletion of either nrg alone. [3] [4]
Strain Background: Sigma1278b (Sigma 2000 series)
Genotype: MCY4597
Together with: NRG2, NRG1, SIP1, SIP2
Phenotype(s): Loss of function (Null)
sip2 gal83 double mutants are defective for filamentation in response to glucose depletion. [3] [4]
Protein Details
Protein Function/Process
One of three alternative beta subunits of the Snf1 kinase complex [5] [6]
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References
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- ↑ Schuller HJ (2003) Transcriptional control of nonfermentative metabolism in the yeast Saccharomyces cerevisiae. Curr Genet 43(3):139-60 SGD PMID 12715202
- ↑ Wiatrowski HA, et al. (2004) Mutations in the gal83 glycogen-binding domain activate the snf1/gal83 kinase pathway by a glycogen-independent mechanism. Mol Cell Biol 24(1):352-61 SGD PMID 14673168
- ↑ 3.00 3.01 3.02 3.03 3.04 3.05 3.06 3.07 3.08 3.09 3.10 3.11 3.12 3.13 3.14 3.15 3.16 3.17 3.18 3.19 3.20 3.21 3.22 3.23 Vyas VK, et al. (2003) Snf1 kinases with different beta-subunit isoforms play distinct roles in regulating haploid invasive growth. Mol Cell Biol 23(4):1341-8 SGD PMID 12556493
- ↑ 4.00 4.01 4.02 4.03 4.04 4.05 4.06 4.07 4.08 4.09 4.10 4.11 4.12 4.13 4.14 4.15 4.16 4.17 4.18 4.19 4.20 4.21 4.22 4.23 submitted by Valmik K. Vyas on 2003-03-27
- ↑ Schmidt MC and McCartney RR (2000) beta-subunits of Snf1 kinase are required for kinase function and substrate definition. EMBO J 19(18):4936-43 SGD PMID 10990457
- ↑ submitted by Martin C. Schmidt on 2003-02-17
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