SGA

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SGA, synthetic genetic array was developed in toronto by Boone and Andrews and their colleauges. it aims to produce doulbe mutants in a genome wide manner for studies such as synthetic lethality and functional genomics.

Details of this method can be found at: http://www.utoronto.ca/boonelab/pdf/SGA_protocol_final.pdf

Robot for this method: Singer Rotor from Singer instruments at http://www.singerinst.co.uk/

Software for this method (scoring program): YEAST-IAE at http://www.lulu.com/product/cd/sga-colony-scoring-software/5361033

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