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Yeastriction is a web-based tool that allows researches to find suitable CRISPR/Cas9 target sites in their strain of interest (the tool now contains 33 different S. cerevisiae strains). The tool helps to select suitable targets and to design primers necessary for construction of plasmids that express specific gRNAs.

In addition to the web-tool, the authors also constructed a set of standardized plasmids and yeast strains (available from EUROSCARF) for expression of one or two gRNAs and explore their use for multiplexed gene deletions, both alone and in combination with multigene chromosomal integrations and/or with the introduction of single-nucleotide changes.

The software and related experimental tools are described in a paper in FEMS Yeast Research.